Publications by authors named "M Arranz Betegon"
The production of Adeno-associated virus (AAV) vectors in the lab setting has typically involved expression in adherent cells followed by purification through ultracentrifugation in density gradients. This production method is, however, not easily scalable, presents high levels of cellular impurities that co-purify with the virus, and results in a mixture of empty and full capsids. Here we describe a detailed AAV production protocol that overcomes these limitations through AAV expression in suspension cells followed by AAV affinity purification and AAV polishing to separate empty and full capsids, resulting in high yields of ultra-pure AAV that is highly enriched in full capsids.
View Article and Find Full Text PDFMisfolded proteins in the endoplasmic reticulum (ER) are returned to the cytosol and destroyed by a process known as ER-associated degradation (ERAD). Hrd1 has been implicated as the channel that mediates the transport of ERAD substrates to the cytosol. A study demonstrates that Hrd1 is gated by autoubiquitination and a soluble ERAD substrate.
View Article and Find Full Text PDFArticle Synopsis
- Traditional methods for cryo-EM often lead to issues like low particle numbers, uneven distribution, biased orientations, and damage due to air-water interfaces.
- Functionalizing graphene oxide (GO) coated grids with amino groups enhances sample concentration, resulting in better distribution and orientation of particles on the grid.
- Adding a PEG spacer helps keep particles away from both the GO surface and the air-water interface, reducing the risk of denaturation.
Inhaled Methoxyflurane Provides Greater Analgesia and Faster Onset of Action Versus Standard Analgesia in Patients With Trauma Pain: InMEDIATE: A Randomized Controlled Trial in Emergency Departments.
Ann Emerg Med
March 2020
Study Objective: The objective of the InMEDIATE study was to evaluate the change in intensity of traumatic pain over the first 20 min in adult patients treated with methoxyflurane versus standard analgesic treatment in Spain. This the first randomized, active-controlled, multicenter trial of methoxyflurane in the emergency setting in Europe.
Methods: This was a randomized, controlled study that enrolled adult patients with acute moderate to severe (score ≥4 on the 11-point Numeric Rating Scale) trauma-associated pain in 14 Spanish emergency departments.
Article Synopsis
- Small heat shock proteins (sHSPs), such as Hsp27, help prevent protein aggregation but their binding sites on client proteins like tau are not well understood.
- Hsp27 specifically targets two aggregation-prone regions of tau using a region in its own structure, indicating that interactions among Hsp27 proteins could compete for binding.
- The weak nature of these interactions suggests that competition for binding sites regulates client binding and the assembly of Hsp27, highlighting the complex interactions that influence its function.