Real-world clinical impact of plasma cell-free DNA metagenomic next-generation sequencing assay.

Objective: To describe the real-world clinical impact of a commercially available plasma cell-free DNA metagenomic next-generation sequencing assay, the Karius test (KT).

Methods: We retrospectively evaluated the clinical impact of KT by clinical panel adjudication. Descriptive statistics were used to study associations of diagnostic indications, host characteristics, and KT-generated microbiologic patterns with the clinical impact of KT.

Tracheostomy Practice in the Italian Intensive Care Units: A Point-Prevalence Survey.

: A tracheostomy is a frequently performed surgical intervention in intensive care units (ICUs) for patients requiring prolonged mechanical ventilation. This procedure can offer significant benefits, including reduced sedation requirements, improved patient comfort, and enhanced airway management. However, it is also associated with various risks, and the absence of standardized clinical guidelines complicates its implementation.

Antimicrobial Residues in Poultry Litter: Assessing the Association of Antimicrobial Persistence with Resistant Strains.

: We set out to evaluate the persistence of sulfachloropyridazine, oxytetracycline, and enrofloxacin in broiler chicken litter following therapeutic oral treatment and its association with the isolation of resistant to these antimicrobials. : Forty broiler chickens were raised under controlled conditions and divided into three experimental groups, each treated with a different antimicrobial, in addition to an untreated/control group. Litter samples were collected post treatment, analyzed by UPLC-MS/MS, and processed for the isolation of .

A Twist on Injection Molding: Injecting Conventional Resin Composites.

Purpose: This article presents a different approach to injectable techniques. This technique uses conventional viscosity materials to achieve maximum strength and esthetics.

Clinical Considerations: The use of high-viscosity material which has the strongest physical properties is desirable since failure of anterior resin composite has been described as fracture and recurrent decay.