Detection of clinically relevant variants in the gene below 10% allelic frequency: A multicenter study by ERIC, the European Research Initiative on CLL.

In chronic lymphocytic leukemia, the reliability of next-generation sequencing (NGS) to detect variants ≤10% allelic frequency (low-VAF) is debated. We tested the ability to detect 23 such variants in 41 different laboratories using their NGS method of choice. The sensitivity was 85.

[Not Available].

This study evaluated the use of ChatGPT among current and former medical students enrolled at Italian universities. The primary goal was to assess its usage and applications in education. Secondary objectives included gathering opinions and satisfaction levels with ChatGPT for educational purposes.

Auto-Abs neutralizing type I IFNs in patients with severe Powassan, Usutu, or Ross River virus disease.

Arboviral diseases are a growing global health concern. Pre-existing autoantibodies (auto-Abs) neutralizing type I interferons (IFNs) can underlie encephalitis due to West Nile virus (WNV) (∼40% of patients) and tick-borne encephalitis (TBE, due to TBE virus [TBEV]) (∼10%). We report here that these auto-Abs can also underlie severe forms of rarer arboviral infections.

A Rich Conformational Palette Underlies Human Ca2.1-Channel Availability.

Depolarization-evoked opening of Ca2.1 (P/Q-type) Ca-channels triggers neurotransmitter release, while voltage-dependent inactivation (VDI) limits channel availability to open, contributing to synaptic plasticity. The mechanism of Ca2.

Voltage-dependent G-protein regulation of Ca2.2 (N-type) channels.

How G proteins inhibit N-type, voltage-gated, calcium-selective channels (Ca2.2) during presynaptic inhibition is a decades-old question. G proteins Gβγ bind to intracellular Ca2.