Publications by authors named "M Alvarez-Rodriguez"
Article Synopsis
- * Studied how testosterone influenced sperm quality during cryopreservation, finding that it negatively impacted acrosome integrity in mouflons and ibexes, especially when combined with the aquaporin blocker phloretin.
- * Concluded that testosterone and the AQP blocker adversely affect sperm cryoresistance, contributing to an understanding of seasonal changes in sperm freezing capacity in ruminants.
This study investigates the impact of bovine serum albumin (BSA) inclusion in the thawing extender on boar sperm quality. Thawing protocols in sperm cryopreservation are vital, yet underexplored. It has been determined that BSA can interact with membranes, stabilizing them and preventing damage during the freezing process, for this reason it could also have a beneficial effect during thawing.
View Article and Find Full Text PDFCryopreservation of porcine spermatozoa is detrimental due to their high sensitivity to cold shock, leading to changes akin to capacitation, known as cryocapacitation. These changes, including the acrosomal reaction, hypermotility induction, and protein phosphorylation, might be influenced by the presence of progesterone in seminal plasma and egg yolk, used in most freezing extenders. We tested the effect of various progesterone concentrations added to the freezing extenders (1, 10, and 100 μg/mL).
View Article and Find Full Text PDFArticle Synopsis
- Despite advances in artificial insemination, 5-10% of selected boar sires remain sub-fertile due to inadequate assessment methods for sperm quality.
- The study emphasizes the need to connect findings from ejaculate analysis, including seminal plasma and microRNAs, with sperm production and fertility to improve reproductive outcomes.
- It highlights the importance of nano-sized extracellular vesicles (sEVs) in mediating sperm and female reproductive tract interactions, stressing their potential in improving cryopreservation techniques and overall fertility through better diagnostic and handling methods.
The freeze-thawing process induces osmotic changes that may affect the membrane domain location of aquaporins' (AQP) in spermatozoa. Recent studies suggest that changes in AQP3 localization allows better sperm osmo-adaptation, improving the cryoresistance. Ultra-rapid freezing is an alternative cryopreservation technique that requires less equipment than conventional freezing, and it is faster, simpler and can be used in the field.
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