Performance of diagnostic assays used to detect Cryptosporidium oocysts in faecal samples of cattle in Kuwait and genotyping of Cryptosporidium species.

Backgroud: Cryptosporidium species are zoonotic protozoan parasites responsible for gastroenteritis in various animals and humans. The diagnosis of Cryptosporidium presents many challenges. This research attempted to match the diagnostic efficiency of the modified Ziehl-Neelsen technique (mZN), immunochromatographic assays (IC), and enzyme-linked immunosorbent assay (ELISA) for the detection of Cryptosporidium in faecal samples of cattle in Kuwait.

Epidemiological and Molecular Study of in Preweaned Calves in Kuwait.

is a worldwide enteric protozoan parasite that causes gastrointestinal infection in animals, including humans. The most notable species is because of its zoonotic importance; it is also the leading cause of cryptosporidiosis in preweaned calves. A cross-sectional study was conducted to determine the prevalence of infection, investigate the potential risk factors, and use molecular diagnosis to identify the predominant spp.

Evaluation of immunochromatography test for detection of four enteropathogens in the feces of sheep and goats in Kuwait.

Background: Diarrhea in newborn small ruminants continues to be the cause of significant financial loss in sheep and goat farms worldwide. Commercial immunochromatographic (IC) assays have been designed and evaluated to be used for the diagnosis of diarrhea in cattle; however, there are no trials to use rapid tests in small ruminants.

Aim: This study was carried out in Kuwait to evaluate the performance of the rapid immunochromatography test (BoviD-4, BioNote, Inc, Korea) for diagnostics of , rotavirus A (RVA), bovine coronavirus (BCoV), and K99 ( K99) in fecal samples of sheep and goats.

Risk factors of diarrhea in small ruminants in Kuwait.

Background: Diarrhea is the most severe disease affecting small ruminant farms, even though there is continuous progress in the control and prevention strategies.

Aims: This study aimed to identify some of the risk factors associated with diarrhea in small ruminants in Kuwait.

Methods: Faecal samples from 556 animals (334 sheep and 222 goats) were collected and examined for different enteropathogens.

Epidemiological observations on cryptosporidiosis and molecular characterization of Cryptosporidium spp. in sheep and goats in Kuwait.

Molecular epidemiological analysis of cryptosporidiosis in Middle Eastern countries suggests that small ruminants could play a major role in the transmission of Cryptosporidium spp. to humans, with a dominance of Cryptosporidium parvum, especially its IId subtypes. However, little information is available on the epidemiology and risk factors of cryptosporidiosis as well the distribution of Cryptosporidium species/genotypes and subtypes in small ruminants in this area, including Kuwait.