Publications by authors named "M AbuOun"
BackgroundAntimicrobial resistance (AMR) is a global threat. Monitoring using an integrated One Health approach is essential to detect changes in AMR occurrence.AimWe aimed to detect AMR genes in pathogenic and commensal collected 2013-2020 within monitoring programmes and research from food animals, food (fresh retail raw meat) and humans in six European countries, to compare vertical and horizontal transmission.
View Article and Find Full Text PDFAntimicrobial resistance (AMR) is a global health hazard. Although clinical and agricultural environments are well-established contributors to the evolution and dissemination of AMR, research on wastewater treatment works (WwTWs) has highlighted their potential role as disseminators of AMR in freshwater environments. Using metagenomic sequencing and analysis, we investigated the changes in resistomes and associated mobile genetic elements within untreated wastewater influents and treated effluents of five WwTWs, and sediments collected from corresponding river environments in Oxfordshire, UK, across three seasonal periods within a year.
View Article and Find Full Text PDFTowards facilitated interpretation of shotgun metagenomics long-read sequencing data analyzed with KMA for the detection of bacterial pathogens and their antimicrobial resistance genes.
Front Microbiol
April 2024
Metagenomic sequencing is a promising method that has the potential to revolutionize the world of pathogen detection and antimicrobial resistance (AMR) surveillance in food-producing environments. However, the analysis of the huge amount of data obtained requires performant bioinformatics tools and databases, with intuitive and straightforward interpretation. In this study, based on long-read metagenomics data of chicken fecal samples with a spike-in mock community, we proposed confidence levels for taxonomic identification and AMR gene detection, with interpretation guidelines, to help with the analysis of the output data generated by KMA, a popular mer read alignment tool.
View Article and Find Full Text PDFArticle Synopsis
- The study aimed to investigate the spread of amikacin-resistant Enterobacteriaceae on a UK pig farm, particularly focusing on Salmonella carrying the rmtB gene.
- Isolates were collected from environmental samples, with real-time PCR and whole-genome sequencing used to analyze the presence of rmtB in both Salmonella and E. coli.
- The findings showed that rmtB was present in multiple strains of Salmonella and E. coli, raising concerns about antibiotic resistance and the importance of ongoing surveillance in agricultural settings.
Article Synopsis
- Surveillance is crucial for tracking the rise of antimicrobial resistance (AMR) in bacteria, especially as it can spread from livestock to humans through the food chain.
- This study used whole genome sequencing to analyze E. coli samples from poultry in the UK, revealing shifts in the dominant AMR genes over time, particularly with increases in diversity by 2020.
- The findings emphasize the importance of ongoing genomic monitoring to understand AMR dynamics and facilitate cross-country comparisons, ultimately aiding in identifying and addressing emerging threats.