Optimizing mouse metatranscriptome profiling by selective removal of redundant nucleic acid sequences.

Unlabelled: Metatranscriptome (MetaT) sequencing is a critical tool for profiling the dynamic metabolic functions of microbiomes. In addition to taxonomic information, MetaT also provides real-time gene expression data of both host and microbial populations, thus permitting authentic quantification of the functional (enzymatic) output of the microbiome and its host. The main challenge to effective and accurate MetaT analysis is the removal of highly abundant rRNA transcripts from these complex mixtures of microbes, which can number in the thousands of individual species.

Multi-omics analysis in inclusion body myositis identifies mir-16 responsible for HLA overexpression.

Background: Inclusion Body Myositis is an acquired muscle disease. Its pathogenesis is unclear due to the co-existence of inflammation, muscle degeneration and mitochondrial dysfunction. We aimed to provide a more advanced understanding of the disease by combining multi-omics analysis with prior knowledge.

A collaborative network analysis for the interpretation of transcriptomics data in Huntington's disease.

Rare diseases may affect the quality of life of patients and be life-threatening. Therapeutic opportunities are often limited, in part because of the lack of understanding of the molecular mechanisms underlying these diseases. This can be ascribed to the low prevalence of rare diseases and therefore the lower sample sizes available for research.

Fracture load of feldspar ceramic crowns: effects of surface treatments and aging.

Objectives: To compare the impact of intaglio surface treatments - airborne particle abrasion and hydrofluoric acid (HF) etching - of feldspar ceramic (FEL) crowns on the fracture load (FL) and to investigate the effects of abutment materials and artificial aging. The aim was to assess whether etching could be replaced by an alternative surface roughening method.

Materials And Methods: FEL crowns had their intaglio surfaces either abraded (25 µm AlO, 0.

Starting postgraduate medical training in general practice with a rotation in general practice - a qualitative study on experiences and effects.

Objective: In Germany, the rotation into the general practitioner's practice (GPP) as part of postgraduate medical training in general practice traditionally takes place at the end of the training period. The aim of this study was to explore possible subsequent effects of beginning training in the GPP from the perspective of general practitioners (GPs) and GP trainees.

Methods: Nationwide, GPs and GP trainees were recruited who started specialization in GP in the GPP.