Hydroxysafflor yellow A (HSYA), a key ingredient in Chinese medicine, exhibits various pharmacological effects, and its absorption and fluorescence properties were studied under different conditions.
The fluorescence intensity of HSYA is significantly influenced by factors like acidity, temperature, viscosity, and surfactants, especially increasing with the presence of borax, which enhances it by 20 times.
The developed borax-HSYA sensitization system is nontoxic to cells and animals, offering a reliable method for studying HSYA's behavior and metabolic processes through fluorescence imaging.