Publications by authors named "M A Wery"

Eukaryotic mRNAs carry an N7-methylguanosine (mG) cap structure at their 5' extremity, which protects them from the degradation by 5'-3' exoribonucleases and plays a pivotal role in mRNA metabolism, promoting splicing, nuclear export, and translation. Decapping, the enzymatic process that removes this structure, is a key event during cytoplasmic mRNA 5'-3' decay, leading to the degradation of the transcript body by Xrn1. In this chapter, we describe a procedure to assess the cap status of RNA at the transcriptome level.

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Northern blotting is a common technique in RNA biology, allowing to detect and quantify RNAs of interest following separation by gel electrophoresis, transfer to a membrane, and hybridization of specific anti-complementary labelled probes. In this chapter, we describe our protocol for efficient RNA extraction from yeast, separation on agarose gel, and capillary transfer to a membrane. We provide two different methods for strand-specific detection of several types of RNAs using oligonucleotide probes, the first using radioactive P-labelled probes, the second based on nonradioactive digoxigenin-labelled probes.

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Saccharomyces cerevisiae is an excellent model to study the effect of external cues on cell division and stress response. 5-Fluorocuracil (5-FU) has been used to treat solid tumors since several decades. The drug was initially designed to interfere with DNA replication but was later found to exert its antiproliferative effect also via RNA-dependent processes.

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Two-component systems (TCSs) are diverse cell signaling pathways that play a significant role in coping with a wide range of environmental cues in both prokaryotic and eukaryotic organisms. These transduction circuitries are primarily governed by histidine kinases (HKs), which act as sensing proteins of a broad variety of stressors. To date, nineteen HK groups have been previously described in the fungal kingdom.

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Article Synopsis
  • Cytoplasmic long noncoding RNAs (lncRNAs), previously thought not to code for proteins, actually interact with ribosomes and their translation is important for their decay.
  • Research shows that Xrn1-sensitive unstable transcripts (XUTs) in yeast are widely translated, affecting their levels through degradation mechanisms.
  • The study finds that the translation process of XUTs is influenced by their 3'-untranslated region length and suggests that the peptides produced from XUTs may be subject to natural selection, with nonsense-mediated decay (NMD) regulating their abundance.
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