Mutations in mexT bypass the stringent response dependency of virulence in Pseudomonas aeruginosa.

Pseudomonas aeruginosa produces a wealth of virulence factors whose production is controlled via an intricate regulatory systems network. Here, we uncover a major player in the evolution and regulation of virulence that enhances host colonization and antibiotic resistance. By characterizing a collection of mutants lacking the stringent response (SR), a system key for virulence, we show that the loss of the central regulator MexT bypasses absence of the SR, restoring full activation of virulence pathways.

Neither increasing the frequency of sow feedings nor decreasing the interval between feedings prior to farrowing reduced piglet stillbirths.

Farrowing durations that exceed 240 min cause stillborn rates to increase. Therefore, feeding strategies in late gestation have been studied to mitigate the negative consequences of extended farrowing durations. A total of 1,501 sows (PIC 1050 Camborough) were used for this study at two individual farms near Carthage, IL.

Positive Attributable Visual Sources Attenuate the Impact of Trigger Sounds in Misophonia.

Misophonia is characterized by strong negative reactions to everyday sounds, such as chewing, slurping or breathing, that can have negative consequences for daily life. Here, we investigated the role of visual stimuli in modulating misophonic reactions. We recruited 26 misophonics and 31 healthy controls and presented them with 26 sound-swapped videos: 13 trigger sounds paired with the 13 Original Video Sources (OVS) and with 13 Positive Attributable Visual Sources (PAVS).

Contribution of the infection ecosystem and biogeography to antibiotic failure in vivo.

The acquisition of antibiotic resistance in bacteria, though a deeply concerning international issue, is reasonably well-understood at a mechanistic level. Less well-understood is why bacteria that are sensitive in vitro to well-established and widely-used antibiotics sometimes fail to respond to these agents in vivo. This is a particularly common problem in chronic, polymicrobial infection scenarios.

The Discovery and Preclinical Profile of ALG-000184, a Prodrug of the Potent Hepatitis B Virus Capsid Assembly Modulator ALG-001075.

Chronic hepatitis B (CHB) represents a significant unmet medical need with few options beyond lifelong treatment with nucleoside analogues, which rarely leads to a functional cure. Novel agents that reduce levels of HBV DNA, RNA and other viral antigens could lead to better treatment outcomes. The capsid assembly modulator (CAM) class of compounds represents an important modality for chronic suppression and to improve functional cure rates, either alone or in combination.