Impact of electro-acupuncture on EAAT2 and NMDAR-2B expression in goats with visceral hypersensitivity.

Objective: This study evaluates the effect of electro-acupuncture (EA) on visceral hypersensitivity (VH) and the expression of N-methyl-D-aspartate receptor-2B (NMDAR-2B) and glutamate transporter EAAT2 in goats.

Methods: Twenty-four goats were divided into four groups: saline, 2, 4, 6-Trinitrobenzenesulfonic acid (TNBS), TNBS + EA, and sham EA. EA was administered at Zusanli (ST36) with 60 Hz and 1-3 mA on specified days.

Two New α-Glucosidase Inhibitors from Haplophyllum tuberculatum: Inhibition Kinetics and Mechanistic Insights Through In vitro and in Silico Approaches.

Two new (1, 2) and nine known (3-11) compounds were isolated from the rutaceous plant Haplophyllum tuberculatum and characterised by extensive NMR spectroscopic techniques and HR-ESI-MS. After structural elucidation, nine compounds were evaluated for their ability to inhibit α-glucosidase, a target for the treatment of type-2 diabetes. Among them, three compounds (7, 5 and 1) exhibited noteable inhbition with IC50 values of 3.

The Correlation of Red Cell Distribution Width With Peripheral Blood Smear: A Study From a Tertiary Care Hospital in Peshawar.

Objective: Anemia is a condition characterized by a shortage of red blood cells (RBCs) and hemoglobin (Hb). A peripheral blood smear (PBS) test involves examining a blood sample to identify important abnormalities in the red blood cells, white blood cells (WBC), and platelets. The aim of this study was to correlate the red cell distribution width (RDW) with peripheral blood smear findings in anemic patients presenting to a tertiary care hospital in Peshawar.

Cardiovascular Effects of Splenomegaly and Splenectomy in Beta-Thalassemia Major.

Background Beta-thalassemia major is a genetic blood disease complicated by splenomegaly, and splenectomy is a standard therapy for this medical condition. Although splenectomy results not only in the improvement of the hematological status, the long-term consequences to the cardiovascular system are still questionable. Objective The aim of the study was to assess and compare the cardiovascular impact of splenomegaly and splenectomy in patients with beta-thalassemia major.

Emerging molecular phenotypes and potential therapeutic targets in esophageal and gastric adenocarcinoma unearthed by whole genome and transcriptome analyses.

Background: Adenocarcinoma of the esophagus and stomach demands a deeper molecular understanding to advance treatment strategies and improve patient outcomes. Here, we profiled the genome and transcriptome landscape of these cancers, explored molecular characteristics that are undetectable by other sequencing platforms, and analyzed their potential clinical ramifications.

Methods: Our study employed state-of-the-art integrative analyses of whole genome and transcriptome sequencing on 51 matched tumor and germline samples from 46 patients.