Publications by authors named "M A QUERE"
Article Synopsis
- Rapid advancements in mRNA technology require new analytical techniques, leading to the development of a novel method using ion-pair reversed-phase liquid chromatography with high-resolution mass spectrometry (IP-RP-LC-HRMS) to analyze poly(A) and poly(T) tail lengths in mRNA's DNA template.
- The study involved validating the poly(A) tail length via Sanger sequencing, amplifying double-stranded DNA using PCR, and confirming sample quality through gel or capillary electrophoresis before extracting and analyzing the tails with LC-MS for precise length determination.
- The results showed that while LC-MS correlated well with Sanger sequencing, it also detected unexpected minor populations of poly(A) tails of varying lengths, suggesting potential
Background: Angumu health zone in Ituri, Democratic Republic of Congo, is a highly malaria-endemic area with an overburdened health system and hosting internally displaced persons (IDP). The World Health Organization recommends mass drug administration (MDA) for malaria in complex emergencies. Therefore, three MDA rounds were implemented by Ministry of Public Health and Médecins sans Frontières from September 2020 to January 2021 in four health areas selected for epidemiological (high malaria incidence) and logistic reasons.
View Article and Find Full Text PDFPreviously, the in vitro growth of cancer stem cells in the form of tumor spheres from five different brain cancer cell lines was found to be methionine-dependent. As this earlier work indicated that , a folate-dependent mitochondria aldehyde dehydrogenase gene, is upregulated in glioblastoma stem cells, we invalidated this gene using CRISPR-cas 9 technique in this present work. We reported here that this invalidation was effective in U251 glioblastoma cells, and no cas9 off target site could be detected by genome sequencing of the two independent knockout targeting either exon I or exon III.
View Article and Find Full Text PDFBackground: Malaria transmission is highly seasonal in Niger. Despite the introduction of seasonal malaria chemoprevention (SMC) in the Magaria District, malaria incidence remains high, and the epidemiology of malaria in the community is not well-understood.
Methods: Four cross-sectional, household-based malaria prevalence surveys were performed in the Magaria District of Niger between October 2016 and February 2018.