Publications by authors named "M A Penalva"
Uso1/p115 and RAB1 tether ER-derived vesicles to the Golgi. Uso1/p115 contains a globular-head-domain (GHD), a coiled-coil (CC) mediating dimerization/tethering, and a C-terminal region (CTR) interacting with golgins. Uso1/p115 is recruited to vesicles by RAB1.
View Article and Find Full Text PDFHyphal tip growth allows filamentous fungi to colonize space, reproduce, or infect. It features remarkable morphogenetic plasticity including unusually fast elongation rates, tip turning, branching, or bulging. These shape changes are all driven from the expansion of a protective cell wall (CW) secreted from apical pools of exocytic vesicles.
View Article and Find Full Text PDFLive-Cell Imaging of Dynein-Mediated Cargo Transport in Aspergillus nidulans.
Methods Mol Biol
January 2023
Filamentous fungi have been used for studying long-distance transport of cargoes driven by cytoplasmic dynein. Aspergillus nidulans is a well-established genetic model organism used for studying dynein function and regulation in vivo. Here, we describe how we grow A.
View Article and Find Full Text PDFArticle Synopsis
- Kinesin-1/KinA transports secretory vesicles (SVs) to the hyphal tip, where they are taken over by type V myosin MyoE for concentration underneath the plasma membrane.
- The stable HUM complex (HMSV-UDS1-MyoE) formed by RAB11 effector UDS1 and HMSV facilitates the interaction between RAB11-GTP and MyoE, crucial for the movement of SVs.
- Disruption of UDS1 or HMSV leads to impaired transport of SVs to the apex, causing the dispersion of RAB11 SVs on the apical surface, indicating the reliance on KinA/microtubule transport mechanisms.