Genomics-based timely detection of dengue virus type I genotypes I and V in Uruguay.

This study details a genomics-based approach for the early detection of mosquito-borne pathogens, marked by Uruguay's first ever complete genomic sequencing of Dengue Virus type I genotypes I and V. This pioneering effort has facilitated the prompt identification of these genotypes within the country, enabling Uruguayan public health authorities to develop timely and effective response strategies. Further integrated into this approach is a climate-driven suitability measure, closely associated with Dengue case reports and indicative of the local climate's role in the virus's transmission in the country within the changing climate context.

The landscape of chemokine and cytokine is associated with the distinct clinical status of leprosy patients and their respective household contacts.

Introduction: Leprosy, a chronic infectious disease, is closely linked to the host immune response. According to the WHO, leprosy patients (L) and household contacts (HHC) are classified into subgroups: paucibacillary (PB) and multibacillary (MB), witch reflect the degree of infection in patients and the level of exposure of their contacts. The main goal of this study was to: i) establish a comprehensive overview of soluble mediator signatures of PBMCs upon antigen-specific stimuli and ii) identify whether the chemokine (CH) and cytokine (CY) signatures were associated with distinct clinical manifestations in (L) and immune response profiles in (HHC).

Influence of the addition of gum arabic and xanthan gum in the preparation of sodium alginate microcapsules coated with chitosan hydrochloride on the survival of Lacticaseibacillus rhamnosus GG.

The microencapsulation of Lactocaseibacillus rhamnosus GG in a matrix of sodium alginate, xanthan gum, gum arabic and chitosan hydrochloride is a promising strategy for protecting this probiotic during passage through the gastrointestinal tract. This study evaluated the influence on the viability of Lactocaseibacillus rhamnosus GG encapsulated with these polymers by external ionic gelation with vibratory extrusion and the microcapsules that showed the best results of capsulation efficiency, viability, size and morphology were analyzed by Fourier transform infrared spectroscopy (FTIR), thermal analysis (TGA) and exposure to environmental stress conditions and gastrointestinal simulation. The result revealed encapsulation efficiency values above 95 % for all formulations and survival rate higher than 6 log CFU/mL for most analyzed groups.

Greater adherence to the Dietary Approaches to Stop Hypertension (DASH) diet during pregnancy reduces the likelihood of having a large-for-gestational-age newborn.

Background/objectives: Studies suggest that greater maternal adherence to the Dietary Approaches to Stop Hypertension (DASH) diet reduces the risk of both maternal and fetal adverse health outcomes. The study aimed to evaluate the relationship between adherence to the DASH diet during pregnancy and the classification of birth weight according to gestational age.

Subjects/methods: Secondary analysis of a prospective cohort of 601 mother and child pairs who attended primary healthcare in a Brazilian municipality.

chooseGCM: A Toolkit to Select General Circulation Models in R.

Studies on climate change need to make projections based on predicted scenarios. One source of variability in these projections is the choice of general circulation models (GCMs). There is a lack of consensus on how to choose the GCMs.