Bioengineered Tumor-Stroma Prostate Cancer In Vitro Models for Screening Therapeutics.

Cancer-associated fibroblasts are increasingly recognized to have a high impact on prostate tumor growth and drug resistance. Here, we bioengineered organotypic prostate cancer 3D in vitro models to better understand tumor-stroma interplay, the metabolomic profile underlying such interactions, and their impact on standard-of-care therapeutics performance. The assembly of robust and uniform spheroids was evaluated and compared in monotypic PC-3 and heterotypic microtumors comprised of either a healthy or malignant stroma and prostate cancer cells.

Alpha-Lipoic Acid Reduces Neuroinflammation and Oxidative Stress Induced by Dapsone in an Animal Model.

Chronic treatment with dapsone (DDS) has been linked to adverse reactions involving all organ systems, such as dapsone hypersensitivity syndrome, methemoglobinemia and hemolytic anemia, besides neuroinflammation and neurodegeneration due to iron accumulation and oxidative stress. These effects probably occur due to the presence of its toxic metabolite DDS-NOH, which can generate reactive oxygen species (ROS) and iron overload. In this sense, antioxidant compounds with chelating properties, such as alpha-lipoic acid (ALA), may be an interesting adjuvant therapy strategy in treating or preventing these effects.

Surgical management and long-term follow-up of ocular hypotony associated with peripheral circumferential retinal detachment.

Purpose: This study aimed to evaluate the surgical management and long-term follow-up of hypotony related to post-vitrectomy peripheral circumferential retinal detachment (PCD).

Methods: Eyes diagnosed with hypotony secondary to PCD after pars plana vitrectomy were retrospectively reviewed. The patient demographic data, complications, management, and treatment outcomes were collected and analyzed.

Eradicating latent tuberculosis: use of interferon gamma release assay and isoniazid/rifapentine in people living with HIV/AIDS.

Tuberculosis (TB) is the most common comorbidity in people living with HIV/AIDS (PLWH), including those under antiretroviral treatment. PLWH are 28 times more likely to develop TB in Brazil, the leading cause of HIV-related deaths globally, with approximately 161,000 reported deaths worldwide in 2023. Early diagnosis of latent tuberculosis infection (LTBI) and prophylactic therapy can reduce TB cases, prevent disease progression, and decrease transmission in high-risk populations.

Physiological Synchrony Analysis in a Multi-Person Setting: Unveiling Connections in Autism Family Therapy.

Interpersonal physiological synchrony (PS) is a process that emerges when the physiological responses of two or more individuals show signs of co-regulation and synchronization. PS shows promising relationships with social behavior, emerging as a potential therapeutic target for populations facing social limitations. Here we propose a case study where we investigate PS in a family with an individual in the autism spectrum (AS) while they undergo a narrative family therapy session.