Structure and function of the geldanamycin amide synthase from Streptomyces hygroscopicus.

Amide synthases catalyze the formation of macrolactam rings from aniline-containing polyketide-derived seco-acids as found in the important class of ansamycin antibiotics. One of these amide synthases is the geldanamycin amide synthase GdmF, which we recombinantly expressed, purified and studied in detail both functionally as well as structurally. Here we show that purified GdmF catalyzes the amide formation using synthetically derived substrates.

Mother's Own Milk Provision During the First 12 Weeks of Life by Gestational Age.

Importance: Although mother's own milk (MOM) is associated with reduced risk of neonatal complications of prematurity and improved neurodevelopmental outcomes, to our knowledge, there have been no large US studies reporting rates of MOM feeding initiation and sustained MOM feedings by gestational age (GA).

Objective: To compare rates of MOM feeding initiation and continuation at 12 weeks for infants by GA.

Design, Setting, And Participants: This retrospective cross-sectional study used state-level surveillance data for maternal characteristics and behaviors before and after pregnancy.

Environmental, Social and Governance principles in Australian publicly funded healthcare: an extension of value-based care.

Australia's publicly funded healthcare system is financed through a complex mix of Commonwealth, State, Territory and private sources, which include activity-based funding, fee for service and block funding. These models may be insufficient to meet future budgetary constraints, and healthcare systems in Australia and worldwide are increasingly adopting value-based healthcare (VBHC), which is defined as 'health outcomes that matter to patients relative to the resources or costs required, over a full cycle of care'. There is no national Australian strategy for the adoption of VBHC, and examining other industries or policies may provide solutions to enhance implementation.

Focal to bilateral tonic-clonic seizures in newly diagnosed focal epilepsy.

Presence of focal to bilateral tonic-clonic seizures (FBTCS) in focal epilepsy is associated with increased morbidity and mortality. Risk factors for FBTCS are poorly understood, and little is known regarding FBTCS recurrence after treatment initiation. This study aimed to investigate factors related to FBTCS in newly diagnosed focal epilepsy and their recurrence after starting antiseizure medications (ASMs) in the Human Epilepsy Project (HEP) cohort.

Influences of motivation and personality on physical activity behavior: A systematic review .

Physical activity, when maintained at specific levels, offers numerous health benefits. However, contemporary trends indicate a decline in physical activity engagement. Interventions incorporating motivation and personality factors present potential methods for practitioners to enhance participation in physical activity behaviors.