Publications by authors named "M A Geurts"
Introduction: The optimal treatment for recurrent glioblastoma patients remains not well-defined in international guidelines. On top of that, the availability of national guidelines is uncharted.
Research Question: This study aimed to investigate the availability of national guidelines on the diagnosis and treatment of adult glioma throughout Europe, specifically focusing on recurrent glioblastoma.
Article Synopsis
- The mammalian pancreas has three key parts: exocrine acini and ducts, along with endocrine islets, all originating from a common progenitor during development.
- Researchers created 18 human fetal pancreas organoid lines from samples between 8-17 weeks of gestation, with four lines showing the ability to produce all three cell types while thriving in culture for over two years.
- Single-cell RNA sequencing revealed LGR5 cells as crucial developmental stem cells, indicating that these organoids are capable of long-term growth and can differentiate into acinar, ductal, and endocrine cells.
Carcinogenesis results from the sequential acquisition of oncogenic mutations that convert normal cells into invasive, metastasizing cancer cells. Colorectal cancer exemplifies this process through its well-described adenoma-carcinoma sequence, modeled previously using clustered regularly interspaced short palindromic repeats (CRISPR) to induce four consecutive mutations in wild-type human gut organoids. Here, we demonstrate that long-term culture of mismatch-repair-deficient organoids allows the selection of spontaneous oncogenic mutations through the sequential withdrawal of Wnt agonists, epidermal growth factor (EGF) agonists and the bone morphogenetic protein (BMP) antagonist Noggin, while TP53 mutations were selected through the addition of Nutlin-3.
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