Distinct MUNC lncRNA structural domains regulate transcription of different promyogenic factors.

Many lncRNAs have been discovered using transcriptomic data; however, it is unclear what fraction of lncRNAs is functional and what structural properties affect their phenotype. MUNC lncRNA (also known as eRNA) acts as an enhancer RNA for the Myod1 gene in cis and stimulates the expression of other promyogenic genes in trans by recruiting the cohesin complex. Here, experimental probing of the RNA structure revealed that MUNC contains multiple structural domains not detected by prediction algorithms in the absence of experimental information.

MUNC, an Enhancer RNA Upstream from the Gene, Induces a Subgroup of Myogenic Transcripts in Independently of MyoD.

MyoD upstream noncoding RNA (MUNC) initiates in the distal regulatory region (DRR) enhancer of and is formally classified as an enhancer RNA (DRR). MUNC is required for optimal myogenic differentiation, induces specific myogenic transcripts in (, , and ), and has a functional human homolog. The vast majority of eRNAs are believed to act in primarily on their neighboring genes (1, 2), making it likely that MUNC action is dependent on the induction of RNA.

Regulation of several androgen-induced genes through the repression of the miR-99a/let-7c/miR-125b-2 miRNA cluster in prostate cancer cells.

The androgen receptor (AR) stimulates and represses gene expression to promote the initiation and progression of prostate cancer. Here, we report that androgen represses the miR-99a/let7c/125b-2 cluster through AR and anti-androgen drugs block the androgen-repression of the miRNA cluster. AR directly binds to the host gene of the miR-99a/let7c/125b-2 cluster, LINC00478.

Histone chaperones Nap1 and Vps75 regulate histone acetylation during transcription elongation.

Histone chaperones function in chromatin assembly and disassembly, suggesting they have important regulatory roles in transcription elongation. The Saccharomyces cerevisiae proteins Nap1 and Vps75 are structurally related, evolutionarily conserved histone chaperones. We showed that Nap1 genetically interacts with several transcription elongation factors and that both Nap1 and Vps75 interact with the RNA polymerase II kinase, CTK1.