Differential adhesion molecule expression on porcine mononuclear cell populations.

Adhesion molecule expression was analysed on porcine blood and lymphoid organ CD4+ CD8 naive T helper (Th) lymphocytes, CD4+CD8+ memory Th lymphocytes (particular to the pig), CD4-CD8high cytotoxic T (Tc) lymphocytes, CD4 CD8low NK cells (CD3- in the pig), CD4-CD8- T-cell receptor-gammadelta-positive (TCRgammadelta+) lymphocytes, B lymphocytes and monocytes. While CD44 expression was relatively homogeneous amongst mononuclear cells, differences were noted for the integrins. Blood naive Th lymphocytes were CD49d(low)CD11a(low), as were splenic naive Th cells; blood memory Th lymphocytes were CD49d(high)CD11a(low), splenic memory Th cells were CD49d(high)CD11a(high) with a CD49d(high)CD11a(low) subpopulation; blood Tc lymphocytes were mainly CD49d(low)CD11a(low), and splenic cells were CD49d(high) CD11a(high).

Lack of association of bovine MHC class I alleles with carcass and reproductive traits.

The present study was carried out to examine whether a relationship between bovine major histocompatibility complex (BoLA) class I alleles and carcass traits or reproductive performance exists in Braunvieh and Fleckvieh AI (artificial insemination) bulls. The influence of BoLA class I (BoLA-A) alleles on deregressed breeding values for net growth rate, carcass index and thigh volume was assessed in Braunvieh crosses and Fleckvieh bulls with a gene substitution model. The reproductive traits: non-return rate and interval between first and last insemination of daughters (female fertility), as well as non-return rate of inseminated cows (male fertility), were only investigated in Fleckvieh animals.

Polymorphism of bovine MHC class II genes. Joint report of the Fifth International Bovine Lymphocyte Antigen (BoLA) Workshop, Interlaken, Switzerland, 1 August 1992.

Polymorphism of the bovine DRB, DQA, DQB, DYA, DOB and DIB genes was investigated using restriction fragment length polymorphism (RFLP) analysis, isoelectric focusing (IEF), class II serology and polymerase chain reaction (PCR) based typing techniques. The simultaneous application of multiple typing techniques and the characterization of multiple genes resulted in a greatly enhanced picture of the bovine class II regions. Thirty-eight class IIa (DR-DQ) and 5 class IIb (DYA-DOB-DIB) haplotypes were defined.

Polymorphism of bovine MHC class I genes. Joint report of the Fifth International Bovine Lymphocyte Antigen (BoLA) Workshop, Interlaken, Switzerland, 1 August 1992.

The objectives of the Fifth International BoLA Workshop were to: standardize nomenclature, compare typing methods, and characterize BoLA haplotypes. The workshop was based on the distribution of blood samples (cells) from 60 selected cattle to 14 laboratories. Results for the class I (BoLA-A) region are presented in this paper while results for the class II regions are presented in a separate report.