Dietary Nigella sativa nanoparticles enhance broiler growth performance, antioxidant capacity, immunity, gene expression modulation, and cecal microbiota during high ambient temperatures.

Environmental heat stress causes significant economic loss in the poultry industry. Therefore, interest has increased in using feed additives to reduce the negative impacts of heat stress on the chickens and improve production performance. This study aimed to assess the effect of supplementing with Nigella sativa nanoparticles (Nano-NS) as an anti-stress and growth promoter in broiler diets under hot climatic conditions.

Characterization of novel cold-active chitin deacetylase for green production of bioactive chitosan.

A Novel cold-active chitin deacetylase from Shewanella psychrophila WP2 (SpsCDA) was overexpressed in Escherichia coli BL21 and employed for deacetylation of chitin to chitosan. The produced chitosan was characterized, and its antifungal activity was investigated against Fusarium oxysporum. The purified recombinant SpsCDA appeared as a single band on SDS-PAGE at approximately 60 kDa, and its specific activity was 92 U/mg.

Laryngopharyngeal reflux: knowledge, attitudes, and practices among primary healthcare physicians.

Objective: The aim of this study was to estimate the level of knowledge, attitudes, and practices regarding laryngopharyngeal reflux (LPR) among primary healthcare (PHC) physicians in Saudi Arabia.

Methods: This quantitative cross-sectional study used an online-based questionnaire distributed to physicians in PHC facilities and hospitals in Saudi Arabia. The questionnaire consisted of four sections that provided data on demographics, knowledge, attitudes, and practices.

Student Performance and Perceptions of Flipped Classrooms and Small Group Discussions as Teaching Tools in Practical Anatomy.

Introduction A quasi-experimental study was conducted to assess students' attitudes toward the flipped practical classroom and evaluate its effectiveness in teaching practical anatomy. Methods Two survey questionnaires were developed: the first assessed students' attitudes toward flipped practical classrooms, while the second focused on the obstacles and difficulties students might encounter in this learning environment. Results We found that students rated flipped learning significantly higher compared to other teaching methods, particularly in terms of the quality of learning materials, enhancement of learning skills, engagement and understanding of anatomy topics, and problem-solving abilities.