[Nucleotide sequence organization of nuclear DNA of wheat embryos].

The kinetic component composition of wheat embryo nuclear DNA was studied. It was shown that 32% of the genome consist of highly repetitive sequences. Intermediate repetitive sequences with repetitive frequency 1300 per genome constitute the bulk (52%) of the wheat embryo nuclear genome.

[Release of informosomes from isolated nuclei of wheat embryos in an in vitro system].

The properties of the informosomes released from isolated nuclei of wheat embryos were studied in an in vitro system. The release of the informosomes from isolated nuclei of wheat embryos labelled with [3H]uridine is stimulated by exogeneous ATP. Optimal conditions for informosomes release from nuclei were determined.

[Proteins of polyribosome-bound informosome of germinating wheat embryos].

Proteins of polyribosome-bound informosomes of germinating wheat embryos were studied by electrophoresis in polyacrylamide gel in presence of sodium dodecyl sulfate. liberation of informosomal proteins was achieved by mild ribonuclease treatment of polyribosomes. It was shown, that proteins of informosomes associated with polyribosomes contain polypeptides with molecular weights of 86 000, 75 000, 72 000, 66 000, 52 000 and 34 000.

[Poly A-protein complexes in polyribosomes of sprouting wheat embryos].

A purified polyribosome preparation has been isolated from sprouting wheat embryos. After treatment of the polyribosomes with pancreatic RNase the poly-A-sequences are found in the fractions sedimenting in the region of 8--14 S. The ability of 8--14 S poly-A-containing complexes to bind to the membrane filters at high ionic strength and their sensitivity to pronase indicate that in sprouting wheat embryo polyribosomes the poly-A-sequences occur as poly-A-protein complexes.

[Poly(A)-containing RNA from germinating wheat embryos].

Rapidly labelled mRNAs were isolated from informosomes and polyribosomes of imbibed wheat embryos. The distribution of poly(A) sequences in these fractions were studied by poly(U) Sepharose chromatography. It was shown that informosomes contain 11% polyadenylated mRNA while polyribosomes--38%.