New 3-(Dibenzyloxyphosphoryl)isoxazolidine Conjugates of N1-Benzylated Quinazoline-2,4-diones as Potential Cytotoxic Agents against Cancer Cell Lines.

In this study, a new series of and 5-substituted-3-(dibenzyloxyphosphoryl)isoxazolidines - were synthesized by the 1,3-dipolar cycloaddition reaction of -benzyl--(dibenzyloxyphosphoryl)nitrone and selected -allyl--benzylquinazoline-2,4-diones. All the obtained -isoxazolidines - and the samples enriched in respective -isomers were evaluated for anticancer activity against three tumor cell lines. All the tested compounds exhibited high activity against the prostate cancer cell line (PC-3).

The Prevalence of Alert Pathogens and Microbial Resistance Mechanisms: A Three-Year Retrospective Study in a General Hospital in Poland.

The development of antibiotic resistance mechanisms hinders the treatment process. So far, there is limited data on the problem of bacterial resistance in hospitals in Central and Eastern Europe. Therefore, this study aimed to assess the prevalence of resistance mechanisms and alert pathogens based on reports regarding cultures of samples collected from general hospital patients in Poland in the period 2019-2021.

Design, Synthesis, Anti-Varicella-Zoster and Antimicrobial Activity of (Isoxazolidin-3-yl)Phosphonate Conjugates of N1-Functionalised Quinazoline-2,4-Diones.

Dipolar cycloaddition of the N-substituted -(diethoxyphosphonyl)nitrones with -allyl--benzylquinazoline-2,4-diones produced mixtures of diastereoisomeric 3-(diethoxyphosphonyl)isoxazolidines with a -benzylquinazoline-2,4-dione unit at C5. The obtained compounds were assessed for antiviral and antibacterial activities. Several compounds showed moderate inhibitory activities against VZV with EC values in the range of 12.

The metabolism of human soluble amyloid precursor protein isoforms is quantifiable by a stable isotope labeling-tandem mass spectrometry method.

Evidence suggests that β-secretase (BACE1), which cleaves Amyloid Precursor Protein (APP) to form sAPPβ and amyloid-β, is elevated in Alzheimer's disease (AD) brains and biofluids and, thus, BACE1 is a therapeutic target for this devastating disease. The direct product of BACE1 cleavage of APP, sAPPβ, serves as a surrogate marker of BACE1 activity in the central nervous system. This biomarker could be utilized to better understand normal APP processing, aberrant processing in the disease setting, and modulations to processing during therapeutic intervention.

Cytokine and LL-37 gene expression levels in Bartonella spp. seropositive and seronegative patients of a rheumatology clinic.

Purpose: The variation in the immune response to Bartonella spp. infection in humans remains unclear. The present study compares the expression of selected interleukins, cytokines and cathelicidin (LL-37) in rheumatology clinic patients suffering from musculoskeletal symptoms with healthy blood donors.

Blood Culture Contamination: A Single General Hospital Experience of 2-Year Retrospective Study.

In the event of blood culture contamination (BCC), blood culture (BC) needs to be repeated. This may delay appropriate treatment, prolong hospitalization and, consequently, increase its costs. The aim of the study was to assess the frequency of BCC and associated factors in a general hospital in Poland based on reports of BC in samples submitted for laboratory testing in 2019−2020.

The seroprevalence of Bartonella spp. in the blood of patients with musculoskeletal complaints and blood donors, Poland: a pilot study.

Background: Bartonella spp. can cause a variety of diseases, such as lymphadenopathies, cat scratch disease, and trench fever, but can also give rise to many non-specific symptoms. No data exists regarding the prevalence of Bartonella spp.

The ability of selected plant essential oils to enhance the action of recommended antibiotics against pathogenic wound bacteria.

The aim of this work was to characterize the ability of essential oils to support antibiotics against pathogenic bacteria in wounds. Gram-positive and Gram-negative bacteria obtained from wound infections were identified according to standard microbiological methods. Essential oils were analysed by GC-FID-MS.

Synthesis, anti-varicella-zoster virus and anti-cytomegalovirus activity of quinazoline-2,4-diones containing isoxazolidine and phosphonate substructures.

Cycloadditions of N-substituted C-(diethoxyphosphoryl)nitrones to N-allylated quinazoline-2,4-diones functionalized at N3 with substituted benzoyl or benzyl groups proceeded with moderate to good diastereoselectivities (d.e. 28-68%).

The Sensitivity of Endodontic Enterococcus spp. Strains to Geranium Essential Oil.

Enterococci are able to survive endodontic procedures and contribute to the failure of endodontic therapy. Thus, it is essential to identify novel ways of eradicating them from infected root canals. One such approach may be the use of antimicrobials such as plant essential oils.

The cultivable microbiota of primary and secondary infected root canals, their susceptibility to antibiotics and association with the signs and symptoms of infection.

Aim: To examine the presence of microbial species in primary and secondary infections and identify the signs and symptoms associated with them.

Methodology: A total number of 37 root canals from 33 patients undergoing root canal treatment were selected. Samples were taken using a sterile paper point following chemomechanical canal instrumentation.

The activity of silver nanoparticles (Axonnite) on clinical and environmental strains of Acinetobacter spp.

Acinetobacter baumannii isolates are responsible for a high number of wound infections. The reason of this study was to evaluate the activity of silver nanoparticles obtained by microexplosion against wide range of Acinetobacter spp. Susceptibility to silver nanoparticles was tested by microdilution method, susceptibility to antibiotics was evaluated by the disc-diffusion method.

Design, synthesis, antiviral, and cytostatic evaluation of novel isoxazolidine analogs of homonucleotides.

Moderate diastereoselectivities (d.e. 2-62%) of isoxazolidine homonucleotides were observed for cycloadditions between N-methyl-C-(diethoxyphosphoryl)nitrone and N-allyl nucleobases, with trans-isoxazolidines predominating.

Frequency of ace, epa and elrA Genes in Clinical and Environmental Strains of Enterococcus faecalis.

Surface proteins play an important role in the pathogenesis of enterococcal infections. Some of them are candidates for a vaccine, e.g.

The potential of use basil and rosemary essential oils as effective antibacterial agents.

The considerable therapeutical problems of persistent infections caused by multidrug-resistant bacterial strains constitute a continuing need to find effective antimicrobial agents. The aim of this study was to demonstrate the activities of basil (Ocimum basilicum L.) and rosemary (Rosmarinus officinalis L.

Properties of acoustic plate modes in YZ LiNbO₃.

Properties of acoustic plate modes (APMs) in YZ LiNbO₃ were calculated and measured. It was found that these modes have a dominant longitudinal component of mechanical motion concentrated near both surfaces of a plate. A delay line with double-electrode interdigital transducers (IDTs) was used for the measurements.

The activity of silver nanoparticles (axonnite) on clinical and environmental strains of Enterococcus spp.

Aims: The spread of bacterial antibiotic resistance among clinical strains is a growing problem nowadays. Resistance in Enterococcus spp. strains has merited special concern of late, as they have become significant nosocomial pathogens.

Antibacterial activity of thyme and lavender essential oils.

Strong antiseptic activity of essential oils has been known for a long time. The antibacterial activity of oils was tested against clinical bacterial strains of Staphylococcus, Enterococcus, Escherichia and Pseudomonas genera. The agar diffusion method was used for microbial growth inhibition at various concentrations of the oils from T.

The antimicrobial activity of thyme essential oil against multidrug resistant clinical bacterial strains.

Aim: The aim of this work was to investigate the antimicrobial activity of thyme essential oil against clinical multidrug resistant strains of Staphylococcus, Enterococcus, Escherichia, and Pseudomonas genus.

Materials: The antibacterial activity of oil was tested against standard strains of bacteria and 120 clinical strains isolated from patients with infections of the oral cavity, abdominal cavity, respiratory and genitourinary tracts, skin, and from the hospital environment.

Methods: Agar diffusion was used to determine the microbial growth inhibition of bacterial growth at various concentrations of oil from Thymus vulgaris.

Determination of mass density, dielectric, elastic, and piezoelectric constants of bulk GaN crystal.

Mass density, dielectric, elastic, and piezoelectric constants of bulk GaN crystal were determined. Mass density was obtained from the measured ratio of mass to volume of a cuboid. The dielectric constants were determined from the measured capacitances of an interdigital transducer (IDT) deposited on a Z-cut plate and from a parallel plate capacitor fabricated from this plate.

Properties of shear horizontal acoustic plate modes in BT-cut quartz.

Properties of shear horizontal acoustic plate modes (SHAPMs) in BT-cut quartz were calculated and measured. A delay line with a long interdigital transducer, deposited on -50.5°YX90°-oriented quartz plate, was used for the measurements.

[Occurrence of virulence genes among Enterococcus faecalis strains isolated from patients and hospital environment].

The aim of this study was to evaluate occurrence of virulence genes among Enterococcus faecalis strains isolated from two hospitals in Łódź, during 2005-2006. The second goal was to determine possible relationship between presence of those genes, resistance to ampicillin and sources of isolation. Enterococcal strains were identified to the species by PCR with ddl primers.