Antibacterial and Antifungal Activity of Metabolites from Basidiomycetes: A Review.

: The search for new antimicrobial molecules is important to expand the range of available drugs, as well as to overcome the drug resistance of pathogens. One of the promising sources of antibacterial and antifungal metabolites is basidial fungi, which have wide biosynthetic capabilities. : The review summarized the results of studying the antimicrobial activity of extracts and metabolites from basidiomycetes published from 2018-2023.

Peroxidase activity of cytochrome c in its compact state depends on dynamics of the heme region.

Cytochrome c (cyt c) is a small globular hemoprotein with the main function as an electron carrier in mitochondrial respiratory chain. Cyt c possesses also peroxidase-like activity in the native state despite its six-coordinated heme iron. In this work, we studied the effect of increasing urea concentration in the range from 0 M to 6 M at pH 7 (pH value of the bulk solvent) and pH 5 (pH value close to negatively charged membrane) on peroxidase-like activity of cyt c.

Subunit analysis of mitochondrial cytochrome c oxidase and cytochrome bc by reversed-phase high-performance liquid chromatography.

A rapid separation of the ten nuclearly-encoded subunits of mitochondrial cytochrome c oxidase, and ten out of the eleven subunits of cytochrome bc, was achieved using a short, 50 mm C-reversed-phase column. The short column decreased the elution time 4-7 fold while maintaining the same resolution quality. Elution was similar to a previously published protocol, i.

Functional and structural evaluation of bovine heart cytochrome c oxidase incorporated into bicelles.

Bilayered long- and short-chain phospholipid assemblies, known as bicelles, have been widely used as model membranes in biological studies. However, to date, there has been no demonstration of structural or functional viability for the fundamental mitochondrial electron transport complexes reconstituted into or interacting with bicelles. In the present work, bicelles were formed from the mixture of long- and short-chain phospholipids, specifically 14:0 and 6:0 phosphatidylcholines (1,2-dimyristoyl-sn-glycero-3-phosphocholine, (DMPC) and 1,2-dihexanoyl-sn-glycero-3-phosphocholine, (DHPC)).

The mechanism of group differences in the character of the vaccinal process in immunization against natural smallpox.

The effect of antigenic polymorphism of the ABO-system blood groups on the character of the vaccinal process after immunization against natural smallpox was investigated. The increased susceptibility of persons possessing A antigen to the harmful effect of smallpox vaccine virus is due to hereditary rather than to acquired factors. The leukocytes of peripheral blood of these persons showed a poorer binding capacity with respect to the smallpoxvaccine virus; they also exhibited a high rate of chromosomal aberration after vaccination, resulting to some extent from increased proliferative ability of the cells.

[Connection between the group factors of the blood systems ABO, MNSs, and rhesus and peculiarities of the vaccination process in children immunized against smallpox].

The ahthors present new data on the character of the vaccine process in children associated with the characteristics of the blood group ABO, MNSs and Rh systems. The greater frequency of occurrence and more manifest reactions were noted in children with blood groups A, B, AB, M and Rho (D) - in comparison with those having blood groups O, Rho (D) +, MN and N. There was a significant prevalence of chromosomal aberrations in the primarily immunized children with blood groups A in comparison with groups O, B and AB.