Experimental infection model for Johne's disease using a lyophilised, pure culture, seedstock of Mycobacterium avium subspecies paratuberculosis.

Johne's disease is a severe chronic enteritis of ruminants caused by Mycobacterium avium subspecies paratuberculosis (Map). Repeatable infections of known duration are required for validation of new diagnostic tests, evaluation of pathogenesis and development of improved vaccines. In the first study of its type, a standardised experimental model for Johne's disease was developed based on a lyophilised, low passage, pure culture, seedstock of Map.

Enzyme-linked immunospot: an alternative method for the detection of interferon gamma in Johne's disease.

To date, the sensitivity of the interferon gamma (IFN-gamma) enzyme-linked immunosorbent assay (ELISA) to detect Johne's disease (JD) has been poor, especially in the early stages of disease. To improve the sensitivity of IFN-gamma detection in the early stages of infection, an alternate assay needs to be developed. The enzyme-linked immunospot (ELISPOT) assay is a highly sensitive technique for the detection of cytokines and has the potential to improve the diagnosis of JD.

The early lymphocyte proliferation response in sheep exposed to Mycobacterium avium subsp. paratuberculosis compared to infection status.

Johne's disease (JD) is an enteric mycobacterial infection of ruminants that has significant global economic impact. Not all individuals exposed to pathogenic mycobacteria succumb to disease. Therefore although early detection of infectious individuals is vital, it is equally important to distinguish how the host response differs in those that are able to successfully clear or control mycobacterial infection and those that are unable to do so.