Conformational changes in the Niemann-Pick type C1 protein NCR1 drive sterol translocation.

The membrane protein Niemann-Pick type C1 (NPC1, named NCR1 in yeast) is central to sterol homeostasis in eukaryotes. NCR1 is localized to the vacuolar membrane, where it is suggested to carry sterols across the protective glycocalyx and deposit them into the vacuolar membrane. However, documentation of a vacuolar glycocalyx in fungi is lacking, and the mechanism for sterol translocation has remained unclear.

Sterol uptake by the NPC system in eukaryotes: a Saccharomyces cerevisiae perspective.

Sterols are an essential component of membranes in all eukaryotic cells and the precursor of multiple indispensable cellular metabolites. After endocytotic uptake, sterols are integrated into the lysosomal membrane by the Niemann-Pick type C (NPC) system before redistribution to other membranes. The process is driven by two proteins that, together, compose the NPC system: the lysosomal sterol shuttle protein NPC2 and the membrane protein NPC1 (named NCR1 in fungi), which integrates sterols into the lysosomal membrane.

Draft Genome Sequences of Seven Type Strains.

In an honors course on "Omics Sciences," draft genome sequences of Chryseobacterium elymi KCTC 22547, Chryseobacterium flavum KCTC 12877, Chryseobacterium hispanicum KCTC 22104, Chryseobacterium lathyri KCTC 22544, " Chryseobacterium massiliae" CCUG 51329, Chryseobacterium piscium CCUG 51923, and Chryseobacterium rhizosphaerae KCTC 22548 were generated to facilitate phylogenomic comparisons within the genus.