Evolutionary and co-evolutionary phage training approaches enhance bacterial suppression and delay the emergence of phage resistance.

The development of phage resistance by bacteria is a major barrier that impedes the therapeutic use of phages. Phage training has been proposed as a novel tool that harnesses the evolutionary potential of phages to improve phage infectivity. Both evolutionary and co-evolutionary phage training models have been previously reported to train phages.

Antidepressants can induce mutation and enhance persistence toward multiple antibiotics.

Antibiotic resistance is an urgent threat to global health. Antidepressants are consumed in large quantities, with a similar pharmaceutical market share (4.8%) to antibiotics (5%).

Microplastics as potential carriers of viruses could prolong virus survival and infectivity.

Microplastics are emerging contaminants in various aquatic environments, leading to human and environmental health concerns. Viruses have also been ubiquitously detected in aquatic environments, and there is an unknown risk of microplastics-mediated virus migration through adsorption. This study applied polystyrene microplastics as the carrier and the T4 bacteriophage (or phage) as the virus model, and a violet side scatter/green fluorescence double-gated flow cytometry approach to investigate the adsorption capacity of viruses on microplastics.

The presence of plasmids in bacterial hosts alters phage isolation and infectivity.

Antibiotic resistance genes are often carried by plasmids, which spread intra- and inter genera bacterial populations, and also play a critical role in bacteria conferring phage resistance. However, it remains unknown about the influence of plasmids present in bacterial hosts on phage isolation and subsequent infectivity. In this study, using both Escherichia coli and Pseudomonas putida bacteria containing different plasmids, eight phages were isolated and characterized in terms of phage morphology and host range analysis, in conjunction with DNA and protein sequencing.

Bacteriophage isolated from non-target bacteria demonstrates broad host range infectivity against multidrug-resistant bacteria.

Antibiotic resistance represents a global health challenge. The emergence of multidrug-resistant (MDR) bacteria such as uropathogenic Escherichia coli (UPEC) has attracted significant attention due to increased MDR properties, even against the last line of antibiotics. Bacteriophage, or simply phage, represents an alternative treatment to antibiotics.