Publications by authors named "Lyle Tobin"
Article Synopsis
- Hydroxyl Radical Protein Footprinting (HRPF) is a technique that analyzes the accessible surfaces of proteins to study their structures and interactions, primarily using hydroxyl radicals generated from hydrogen peroxide.
- A new approach successfully applies this method to whole mammalian blood, overcoming challenges posed by UV absorption that hindered previous uses in tissue analysis.
- The study reports minimal impact on blood cell morphology during the labeling process and outlines an enhanced protocol to reduce unwanted background labeling, paving the way for structural proteomics in medical research.
Purpose: Cytoskeletal protein ensembles exhibit emergent mechanics where behavior exhibited in teams is not necessarily the sum of the components' single molecule properties. In addition, filaments may act as force sensors that distribute feedback and influence motor protein behavior. To understand the design principles of such emergent mechanics, we developed an approach utilizing QCM-D to measure how actomyosin bundles respond mechanically to environmental variables that alter constituent myosin II motor behavior.
View Article and Find Full Text PDFA quartz crystal microbalance (QCM) is an instrument that has the ability to measure nanogram-level changes in mass on a quartz sensor and is traditionally used to probe surface interactions and assembly kinetics of synthetic systems. The addition of dissipation monitoring (QCM-D) facilitates the study of viscoelastic systems, such as those relevant to molecular and cellular mechanics. Due to real-time recording of frequency and dissipation changes and single protein-level precision, the QCM-D is effective in interrogating the viscoelastic properties of cell surfaces and in vitro cellular components.
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