An Update on Glutathione's Biosynthesis, Metabolism, Functions, and Medicinal Purposes.

Glutathione (GSH) has been the focus of increased scientific interest in the last decades. It plays a crucial role in all major physiological processes by supplying antioxidant defenses through participating in cellular redox reactions in the human body and other living organisms. GSH also participates in detoxifying xenobiotics, protecting protein thiols from crosslinking and oxidation, regulating the cell cycle, storing cysteine, etc.

[VIOLATION OF THE PROOXIDANT-ANTIOXIDANT BALANCE IN THE SPLEEN TISSUE UNDER EXPERIMENTAL CARCINOGENESIS].

This study was devoted to the investigation of antioxidant homeostasis in spleen tissue of white rats in the dynamics of development of colon adenocarcinoma induced by the introduction of sym-dimethylhydrazine (DMH). The effectiveness of the antioxidant barrier (Cu,Zn-superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, reduced glutathione) and oxidative damage products (TBARS, advanced oxidation protein products, diene and triene conjugates, Schiff bases) were measured in the homogenate of spleen tissue. A violation of the redox balance due to the accumulation of lipid peroxidation products, a decrease in the activity of antioxidant enzymes and antioxidant mediators of non-enzymatic nature was established.

State of humoral immunity and cytokine profile in rats under experimental carcinogenesis with applying enterosorporation chemotherapeutic factors.

Unlabelled: The number of cancer patients is growing in all countries. Due to the malignant progression of cancer, inflammatory processes are observed, which are inextricably linked with the response of the immune system.

Aim: The aim of this research was to study the effectiveness of the cytostatic Vincristine on the background of sorption correction of disorders by the carbon sorbent AUT-M (activated carbon tissue material) in adenocarcinoma of the colon.

[PRODUCTION OF REACTIVE OXYGEN SPECIES AND DEVELOPMENT OF APOPTOSIS IN BLOOD LEUKOCYTES IN EXPERIMENTAL ANTIPHOSPHOLIPID SYNDROME].

The aim of the study is to investigate the processes of apoptosis and the level of formation of reactive oxygen species (ROS) in blood cells of BALB/c female mice in experimental antiphospholipid syndrome (APS). The content of living, apoptotic and necrotic cells in blood leukocytes was evaluated. Apoptosis in cells was detected using Annexin V conjugated to GFP and propidium iodide.

CHANGES OF CYTOLYSIS INDICATORS IN RATS' BLOOD RESULTED FROM SIMULTANEOUS INTOXICATION WITH TOBACCO SMOKE AND SODIUM NITRITE AFTER USING MILDRONATE.

The article is devoted to an experimental study of the effect of tobacco smoke and the effect of sodium nitrite on the background of tobacco intoxication on the body of rats of different age groups and to identify violations caused by these toxicants. It has been established that under the conditions of nitrite-tobacco intoxication, a more pronounced cytolysis of the cells takes place, which leads to a change in the permeability of the plasma membranes than in case of poisoning with only tobacco smoke. Immature rats were the most sensitive to the action of toxicants, in which the activity of membrane-dependent enzymes was more statistically significant (ALT activity in the serum increased 7,7 times, AST - 5 times, GGTP - 4,1 times, LDH - 1,8 times; p ≤ 0.

EFFECT OF TOBACCO SMOKE ON ROS PRODUCTION AND INFLAMMATION IN RATS OF DIFFERENT AGE.

The aim of our study was to investigate the effect of tobacco smoke on the content of reactive oxygen species (ROS) and level of cytokines in blood of rats of different age groups. For the purpose of carrying out the dosage, the beards of the white mongrel male rats were sub-divided into three groups: immature, mature and senile rats. Rat experimental groups were exposed to tobacco smoke daily for 45 days.