sp. nov., isolated from the tick .

Recently, we obtained a rickettsial isolate ( sp. UFMG-EV) from the haemolymph of engorged tick females. On the basis of maximum-likelihood phylogenetic analysis using 16S rRNA gene, , , and sequences we showed that sp.

Detection of genetic diversity of Anaplasma marginale isolates in Minas Gerais, Brazil.

Bovine anaplasmosis, caused by the tick-borne rickettsia Anaplasma marginale, is endemic in tropical and subtropical regions of the world and results in economic losses in the cattle industry. Major surface proteins (MSPs) have been used as markers for the genetic characterization of A. marginale strains and demonstrate that many isolates may occur in a given geographic area.

Ultrastructure of Ehrlichia mineirensis, a new member of the Ehrlichia genus.

Recently, we reported the in vitro isolation and the molecular characterization of a new species of Ehrlichia (Ehrlichia mineirensis) from haemolymph of Brazilian Rhipicephalus (Boophilus) microplus ticks. This organism shows an ortholog of Ehrlichia canis major immunogenic protein gp36 with a new structure of tandem repeats. In the present study, we used electron microscopy (high pressure freezing and freeze substitution preparative techniques) to characterize morphologically this new agent growing in IDE8 tick cells.

New species of Ehrlichia isolated from Rhipicephalus (Boophilus) microplus shows an ortholog of the E. canis major immunogenic glycoprotein gp36 with a new sequence of tandem repeats.

Background: Ehrlichia species are the etiological agents of emerging and life-threatening tick-borne human zoonoses that inflict serious and fatal infections in companion animals and livestock. The aim of this paper was to phylogeneticaly characterise a new species of Ehrlichia isolated from Rhipicephalus (Boophilus) microplus from Minas Gerais, Brazil.

Methods: The agent was isolated from the hemolymph of Rhipicephalus (B.

Occurrence of ectoparasites on dogs in rural regions of the state of Minas Gerais, Brazil.

The present study examined occurrences of ectoparasites and identified them on dogs in rural regions in Brazil, and assessed the influence of climate on these parasites. Ectoparasites were randomly collected from 194 dogs living on farms located in Lavras (n = 92) and Nanuque (n = 102) during the dry season. During the subsequent rainy season, the same dogs in Lavras (n = 71) and Nanuque (n = 66) were resampled.

In vitro cultivation of Anaplasma marginale and A. phagocytophilum in tick cell lines: a review.

Continuous cell lines have been established from several ixodid and argasid tick species, representing an excellent tool suitable for the isolation of pathogens and their subsequent propagation, which in turn allows the production of antigenic material for diagnostic tests, antibody and vaccine production, and also for studies on host-vector-pathogen relationships. This paper reviews the use of tick cells for culture initiation and maintenance of two obligate intracellular bacterial pathogens, Anaplasma marginale and Anaplasma phagocytophilum. These in vitro cultivation systems have been used in a wide range of studies, covering morphological ultrastructural analysis, genetics, proteomics and biological differences between strains, including genome transcriptional and protein expression approaches, enabling comparisons between host and vector cells.

Survey of pyrethroid and organophosphate resistance in Brazilian field populations of Rhipicephalus (Boophilus) microplus: detection of C190A mutation in domain II of the para-type sodium channel gene.

The cattle tick Rhipicephalus (Boophilus) microplus causes expressive damage to livestock in Brazil and other countries. Its control is becoming more difficult due to the development of resistance in populations. Early detection of resistance can help in developing effective control strategies.

Genetic variants of Anaplasma phagocytophilum in wild caprine and cervid ungulates from the Alps in Tyrol, Austria.

The occurrence of genetic variants of Anaplasma phagocytophilum was studied in wild ungulates from the northern and central eastern Alps in Tyrol, Austria. For this purpose, spleen samples collected from 53 game animals during the hunting season 2008/2009 (16 roe deer [Capreolus capreolus], 10 red deer [Cervus elaphus], 16 Alpine chamois [Rupicapra r. rupicapra], 7 Alpine ibex [Capra i.

Rickettsia felis in fleas, Germany.

Among 310 fleas collected from dogs and cats in Germany, Rickettsia felis was detected in all specimens (34) of Archaeopsylla erinacei (hedgehog flea) and in 9% (24/226) of Ctenocephalides felis felis (cat flea). R. helvetica was detected in 1 Ceratophyllus gallinae (hen flea).

Sero-prevalence and risk indicators for canine ehrlichiosis in three rural areas of Brazil.

Ehrlichia canis has a worldwide geographic distribution, occurring particularly in tropical and subtropical areas. In Brazil, the main vector in urban areas is believed to be the brown dog tick Rhipicephalus sanguineus, but little is known about the occurrence, transmission and other epidemiological aspects of canine ehrlichiosis in rural areas, where Amblyomma ticks are found more frequently than R. sanguineus.

First molecular detection of Babesia vogeli in dogs from Brazil.

The present work describes the detection and first molecular characterization of Babesia vogeli in dogs, naturally infected in Brazil and even in South America. Microscopic examination of Giemsa-stained peripheral blood smears collected from dogs originating from four different locations in Brazil revealed the presence of large Babesia merozoites and trophozoites (>2.5 microm).

Retrospective study (1998-2001) on canine babesiosis in Belo Horizonte, Minas Gerais, Brazil.

The present work describes a retrospective study of clinical cases of babesiosis in dogs examined at the Veterinary Hospital (Universidade Federal de Minas Gerais) from March 1998 to September 2001. From the clinical records of dogs with laboratory-confirmed Babesia canis infections, we analyzed: demography (age, breed, sex, time of year, and origin; clinical manifestations (concomitant infections, body temperature, presence of ticks, and clinical signs); and hematological alterations. From 194 records from animals with suspicious cases of hemoparasites, 145 were confirmed to be infected and among those 61 dogs (42%) were infected with B.