Comparison of Environmental and Culture-Derived Bacterial Communities through 16S Metabarcoding: A Powerful Tool to Assess Media Selectivity and Detect Rare Taxa.

To compare environmental and culture-derived microbial communities, we performed 16S metabarcoding of uncultured samples and their culture-derived bacterial lawns. Microbial communities were obtained from freshwater river samples representative of an anthropization gradient along a river stream. Their culture-derived bacterial lawns were obtained by growing aliquots of the samples on a broad range medium and on two different semi-selective media.

Manipulation of ABA Content in Modifies Sensitivity and Oxidative Stress Response to and Influences Peroxidase Activity.

The production of reactive oxygen species (ROS) is one of the first defense reactions induced in in response to infection by the pectinolytic enterobacterium . Previous results also suggest that abscisic acid (ABA) favors multiplication and spread into its hosts. Here, we confirm this hypothesis using ABA-deficient and ABA-overproducer plants.