Development of a fluorescence-based microtiter plate method for the measurement of glutathione in yeast.

The present work was aimed to the development of a fluorescence assay using the universal 96-well microplate format, for the measurement of reduced glutathione (GSH) in yeast cells. The method relies upon the reaction between GSH and a highly selective fluorogenic probe, i.e.

Potential relationship between glutathione metabolism and flocculation in the yeast Kluyveromyces lactis.

Reduced glutathione (GSH) is involved in biochemical and physiological processes in cells. Flocculation is an important mechanism in microorganisms. The present study concerned the potential relationship between GSH metabolism and flocculation.