Impact of simulated acid rain on the growth of three species Brassica integrifolia, Brassica rapa, Brassica juncea in Hanoi, Vietnam.

Over the past decade, the extent and magnitude of acid rain in Vietnam and other Asian countries have become more apparent. In this study, the effect of simulated acid rain (pH 5.0, 4.

Polyacrylate crosspolymer-11 enhances soil clay dispersibility: An indication for inadvertent environmental impacts from personal care and cosmetic ingredients.

Polymer ingredients are commonly found in personal care and cosmetic industry and their "leakages" into the environment have recently been warned as an emerging environmental risk. Daily use of personal care and cosmetic products can inadvertently release large amounts of polymer ingredients into water courses. However, their fate and interaction with other constituents, especially colloids (e.

A pooled CRISPR/AsCpf1 screen using paired gRNAs to induce genomic deletions in Chinese hamster ovary cells.

Chinese hamster ovary (CHO) cells are the most widely used host for the expression of therapeutic proteins. Recently, significant progress has been made due to advances in genome sequence and annotation quality to unravel the black box CHO. Nevertheless, in many cases the link between genotype and phenotype in the context of suspension cultivated production cell lines is still not fully understood.

Fungicide application can intensify clay aggregation and exacerbate copper accumulation in citrus soils.

Fungicide application for controlling fungal diseases can increase copper (Cu) accumulation in soil. More urgently, Cu released from fungicides can associate with soil clay and favour the mutual aggregation of Cu and soil clay, thereby potentially intensifying the accumulation of Cu. We investigated the effects of Cu salt and six common Cu-based fungicides on colloidal dynamics of a clay fraction from citrus cultivated soil.

Enhanced targeted DNA methylation of the CMV and endogenous promoters with dCas9-DNMT3A3L entails distinct subsequent histone modification changes in CHO cells.

With the emergence of new CRISPR/dCas9 tools that enable site specific modulation of DNA methylation and histone modifications, more detailed investigations of the contribution of epigenetic regulation to the precise phenotype of cells in culture, including recombinant production subclones, is now possible. These also allow a wide range of applications in metabolic engineering once the impact of such epigenetic modifications on the chromatin state is available. In this study, enhanced DNA methylation tools were targeted to a recombinant viral promoter (CMV), an endogenous promoter that is silenced in its native state in CHO cells, but had been reactivated previously (β-galactoside α-2,6-sialyltransferase 1) and an active endogenous promoter (α-1,6-fucosyltransferase), respectively.

Effects of CO and temperature on phytolith dissolution.

Phytoliths, silica structures derived from plant residues in silicon (Si)-accumulating plant species, have recently been recognized as a sink and source of nutrients and a hosting phase for carbon sequestration in soil. While the solubility of phytoliths in relation to their respective nature and solution chemistry has been intensively studied, the combined effects of CO and temperature, two highly variable parameters in soil, have not been fully understood. We hypothesized that changes in CO and temperature may affect the dissolution rate, thereby resizing the soil phytolith pool.

Canopy parkour: movement ecology of post-hatch dispersal in a gliding nymphal stick insect, .

For flightless arboreal arthropods, moving from the understory into tree canopies is cognitively and energetically challenging because vegetational structures present complex three-dimensional landscapes with substantial gaps. Predation risk and wind-induced perturbations in the canopy may further impede the movement process. In the Australian stick insect , first-instar nymphs hatch on the forest floor and disperse toward tree canopies in the daytime.

Bioinformatic Identification of Chinese Hamster Ovary (CHO) Cold-Shock Genes and Biological Evidence of their Cold-Inducible Promoters.

Lower cultivation temperature dramatically affects cell growth and cellular productivity in Chinese hamster ovary cells (CHO) and is often used in industrial applications with the aim to enhance productivity. Cold-inducible proteins whose activity is induced at lower temperature play an important role in understanding the mechanisms of cold-induced changes in gene expression. One of these mechanisms is increased transcription of specific target genes controlled by sequence elements in cold-inducible promoters.

Novel Promoters Derived from Chinese Hamster Ovary Cells via In Silico and In Vitro Analysis.

For the industrial production of recombinant proteins in mammalian cell lines, a high rate of gene expression is desired. Therefore, strong viral promoters are commonly used. However, these have several drawbacks as they override cellular responses, are not integrated into the cellular network, and thus can induce stress and potentially epigenetic silencing.