Assessing hERG channel inhibition using PatchXpress.

PatchXpress, an automated 16-channel parallel patch clamp system, was used to determine inhibition of human ether-a-go-go related gene (hERG) potassium channels by known blockers. A monoclonal cell line stably expressing hERG potassium channels was generated in CHO-K1 cells. Results were compared to conventional patch clamp experiments using similar voltage protocols and solutions.

The accuracy of magnetic resonance imaging in the assessment of glenohumeral articular degenerative disease.

Degenerative joint disease is a major source of disability in the world with over 43 million individuals suffering from the affliction in the United States alone. It is the most common cause of activity limitation in individuals over 65 years of age. While much of the focus in recent years has been on osteoarthritis of the hips and knees, shoulder degenerative disease is becoming a more commonly recognized source of morbidity with a wide range of associated lifestyle-limiting disabilities.

Radiology corner. Answer to last month's radiology case and images: Schistosomiasis japonicum involving the liver and colon.

Abdominal radiography showed subtle curvilinear densities overlying the left lower quadrant and central within the pelvis (Fig. 1a). This was confirmed on intravenous contrast-enhanced abdominopelvic CT to be caused by thin mucosal surface calcifications within the descending and sigmoid colons (Fig.

Infected dermoid sinus tract mimicking recurrent sinusitis in an adolescent male.

Nasal dermoid sinus cysts are the most common congenital midline nasofrontal masses, but they can easily be confused with simple recurrent sinusitis upon clinical presentation. We report the case of a 14-year-old male who was initially diagnosed with frontal sinusitis and treated with antibiotics but, after multiple episodes of recurrence, was later sent for imaging. Computed tomography and magnetic resonance imaging showed an infected dermoid sinus tract.

Synovial Hemangioma of the Knee: A Case Report.

We present a case report of a rare synovial hemangioma of the knee. Magnetic resonance imaging (MRI) demonstrated typical signal characteristics and enhancement. Histologic findings were confirmatory.

Magnetic resonance imaging of the rotator cuff interval.

The rotator cuff interval (RCI) is an important and anatomically complex region of the rotator cuff that is critically important to normal glenohumeral function. Recognition of common pathologies in this region on imaging examinations is especially important as injuries may be difficult to detect on clinical examination and even at arthroscopy. Familiarity with the magnetic resonance imaging appearance of the normal and abnormal RCI and the ability to convey findings to orthopedic and sports medicine referrers are essential to facilitate prompt creation of effective treatment plans.

Gnathodiaphyseal dysplasia.

Gnathodiaphyseal dysplasia (GDD) is an orthopedic condition involving cemento-osseous lesions of jaw bones, sclerosis, bowing of tubular bones, and overall bone fragility. This is a case report of GDD in which an 18-year-old female presented with a several-year history of bony abnormalities of the jaw and long bones. Radiographs demonstrated endosteal thickening, bowing of long bones, and abnormalities of the skull.

Impact of augmenting dialysis frequency and duration on cardiovascular function.

Conventional hemodialysis (CHD) only delivers 10% to 15% of renal function in a nonphysiological intermittent mode. Because it occurs nightly and is sustained over a longer dialysis time, the uremic clearance provided by nocturnal hemodialysis (NHD) far exceeds that of CHD. Increasing the dose and frequency of dialysis by NHD has been demonstrated, in both short- and long-term studies, to reverse several important risk factors for adverse cardiovascular events in patients with end-stage renal disease such as hypertension, left ventricular hypertrophy, systolic dysfunction, conduit artery stiffness, attenuated baroreflex regulation of heart rate, disturbed heart rate variability, sleep apnea, and endothelium-dependent vasodilation.

Carpal tunnel syndrome resulting from hydroxyapatite deposition.

Hydroxyapatite deposition disease (HADD) is a rare cause of carpal tunnel syndrome. This is a case report of a 55-year-old male who presented with symptoms of carpal tunnel syndrome and was subsequently found to have HADD. The diagnosis of HADD is made by the identification of poorly defined cloudy calcifications on radiography of the hand and wrist.

Transparent, conductive, and flexible carbon nanotube films and their application in organic light-emitting diodes.

We have carried out comparative studies on transparent conductive thin films made with two kinds of commercial carbon nanotubes: HiPCO and arc-discharge nanotubes. These films have been further exploited as hole-injection electrodes for organic light-emitting diodes (OLEDs) on both rigid glass and flexible substrates. Our experiments reveal that films based on arc-discharge nanotubes are overwhelmingly better than HiPCO-nanotube-based films in all of the critical aspects, including surface roughness, sheet resistance, and transparency.

Neuroprotection and thrombolysis: combination therapy in acute ischaemic stroke.

The administration of oral aspirin within 48 h and tissue plasminogen activator within 3 h of ischaemic stroke onset remain the only treatments that have been shown to have clinical benefit. There has been much excitement about neuroprotection over the last two decades, as it may minimise the harmful effects of ischaemic neuronal damage. Although each step along the ischaemic cascade offers a potential target for therapeutic intervention, and neuroprotection has shown benefit in animal studies, this has been difficult to translate to humans.

Cavernous angioma of the cerebellopontine angle.

Although cavernous hemangiomas are commonly found supratentorially, their presence in the internal auditory canal is uncommon. We describe a case that was initially mistaken for a vestibular schwannoma due to its location in the cerebellopontine angle. Although there can be overlap in their presentations, familiarity with the characteristic magnetic resonance imaging findings of both entities will aid in their differentiation and potentially affect preoperative planning.

Multiple focal nodular hyperplasia: atypical imaging characteristics in the setting of hepatic steatosis.

Focal nodular hyperplasia is an uncommon, benign lesion of the liver most often affecting women of childbearing age. It can present clinically with chronic, intermittent right upper quadrant pain and nausea, with associated liver enzyme abnormalities. The presence of characteristic sonographic and computed tomography imaging findings can help to differentiate this entity from other hepatic masses.

Postoperative imaging of the hip.

The number and type of operative procedures involving the hip continue to increase, placing a greater emphasis on characterizing patient postoperative condition accurately. Optimal postoperative imaging evaluation may involve multiple modalities, including conventional radiography, radionuclide scintigraphy, and cross-sectional imaging. Many of the surgical procedures involve the placement of metallic joint replacements or fixation that can make the imaging evaluation of the postoperative anatomy challenging.

The K65R reverse transcriptase mutation in HIV-1 reverses the excision phenotype of zidovudine resistance mutations.

The HIV-1 nucleoside reverse transcriptase inhibitors (NRTIs) tenofovir (TFV), abacavir, didanosine and stavudine can select for K65R, whereas zidovudine (AZT) and stavudine can select for thymidine analogue mutations (TAMs) in HIV-1 reverse transcriptase (RT). HIV-1 with TAMs shows reduced susceptibility to all NRTIs, most notably AZT, whereas HIV-1 with K65R shows reduced susceptibility to all NRTIs except AZT. K65R and TAMs rarely occur together in patients.

Intervertebral disk cyst: a case report.

This case describes a patient who presented with chronic low back pain which developed over the course of 1 year. Imaging revealed a 1.1-cm cystic lesion at the L4-L5 vertebral level posterior to the intervertebral disk.

Ischemic thresholds for gray and white matter: a diffusion and perfusion magnetic resonance study.

Background And Purpose: Although gray matter (GM) and white matter (WM) have differing neurochemical responses to ischemia in animal models, it is unclear whether this translates into differing thresholds for infarction. We studied this issue in ischemic stroke patients using magnetic resonance (MR) techniques.

Methods: MR studies were performed in patients with acute hemispheric ischemic stroke occurring within 24 hours and at 3 months.

Drivers of germ cell maturation.

Spermatogenesis requires progression of germ line stem cells through a precisely ordered differentiation pathway to form spermatozoa. Diverse and dynamic signals from the transforming growth factor-beta (TGF-beta) superfamily influence many stages of germ cell development. For example, interactions between several TGF-beta superfamily ligands (bone morphogenetic proteins, activin, and glial-derived neurotrophic growth factor [GDNF]) appear to govern the onset of spermatogenesis, and we are exploring how germ cells interpret these competing signals.

A combination of decreased NRTI incorporation and decreased excision determines the resistance profile of HIV-1 K65R RT.

Objective: To determine the mechanisms of resistance of K65R mutant reverse transcriptase (RT) to the currently approved nucleoside and nucleotide RT inhibitors (NRTI).

Methods: Susceptibilities of K65R mutant HIV-1 to NRTI were determined in cell culture. The Ki/Km values were measured to determine the relative binding or incorporation of the NRTI, and ATP-mediated excision of incorporated NRTI was measured to determine NRTI stability as chain terminators.