Machine learning-assisted washing-free detection of extracellular vesicles by target recycling amplification based fluorescent aptasensor for accurate diagnosis of gastric cancer.

Extracellular vesicles (EVs) are promising non-invasive biomarkers for cancer diagnosis. EVs proteins play a critical role in tumor progress and metastasis. However, accurately and reliably diagnosing cancers is greatly limited by single protein marker on EVs.

Comparations of efficacy and safety of rituximab, calcineurin inhibitors and cyclophosphamide in primary membranous nephropathy: a single-center retrospective analysis.

Background: To compare the efficacy and safety of rituximab (RTX), calcineurin inhibitor (CNI) and cyclophosphamide (CTX) plus glucocorticoids in the treatment of primary membranous nephropathy (PMN).

Methods: Totally 478 biopsy-proven PMN patients in single center were retrospectively included. After 1:1 propensity score matching (PSM), 258 patients were included in RTX, CNI or CTX group (86 patients in each group).

Coupling with in-situ electrochemical reactive chlorine species generation and two-phase partitioning method for enhanced microalgal biodiesel production.

Microalgal-based biofuel production is of great significance in alleviating energy crisis and achieving carbon neutrality. However, the excessive costs and high solvent consumption in lipids extraction from microalgal obstruct the widespread application of biodiesel in practice. Reported herein is the construction of facile strategy for lipids extraction via electrocatalytic pretreatment and a subsequent two-phase partitioning method.

Water-plasma-enhanced and phase-separation-assisted extraction of microalgal lipid for biodiesel production.

Traditional methods for lipid extraction from microalgal biomass usually involve harsh reaction conditions or the use of contaminant reagents, which lead to enormous energy consumption and wastage. Hence, a novel strategy was presented, which combined water-plasma and three-phase partitioning (TPP) techniques. Benefiting from its unique advantages such as rapid and low cost, water-plasma strategy can disrupt microalgal cell wall and can thus greatly affect lipid extraction.

Alternative Splicing: A New Cause and Potential Therapeutic Target in Autoimmune Disease.

Alternative splicing (AS) is a complex coordinated transcriptional regulatory mechanism. It affects nearly 95% of all protein-coding genes and occurs in nearly all human organs. Aberrant alternative splicing can lead to various neurological diseases and cancers and is responsible for aging, infection, inflammation, immune and metabolic disorders, and so on.

Noise exposure in occupational setting associated with elevated blood pressure in China.

Background: Hypertension is the primary out-auditory adverse outcome caused due to occupational noise exposure. This study investigated the associations of noise exposure in an occupational setting with blood pressure and risk of hypertension.

Methods: A total of 1,390 occupational noise-exposed workers and 1399 frequency matched non-noise-exposed subjects were recruited from a cross-sectional survey of occupational noise-exposed and the general population, respectively.

Structure-based functional design of chemical ligands for AMPA-subtype glutamate receptors.

Glutamate receptors (GluRs) function as transmembrane ion channels to regulate intracellular level of ions such as calcium in control of excitatory synaptic transmission of the central nervous system. Dysfunction of these glutamate receptors has been implicated in human brain neurodegenerative diseases, including Alzheimer's, Huntington's, and Parkinson's diseases. Despite such a significant role in both the biology and pathology of the central nervous system, detailed understanding of molecular mechanisms by which subtype- or subunit-specific glutamate receptors function in cells is still lacking.