Disrupted maxillofacial, cardiovascular, and nervous development in washc5 knockout Zebrafish: Insights into 3C syndrome.

3C syndrome features craniofacial, nervous, and cardiovascular malformations. WASHC5 gene mutations may underline this syndrome, but the pathogenicity and underlying mechanism remain undetermined. We analyzed the expression pattern of the washc5 gene in zebrafish using whole-body in situ hybridization and generated a zebrafish model with washc5 gene knockout using CRISPR/Cas9 technology.

Mechanochemical conversion of chitin to high-bioactivity oligomers of N-acetyl-D-glucosamine.

Organonitrogen chemicals are crucial in pharmaceutical production and material synthesis. However, the current overreliance on the energy-intensive and highly polluting Haber-Bosch synthesis process necessitates an urgent need to explore more sustainable and environmentally friendly methods for producing these chemicals. Herein, we report an effective method for the selective conversion of chitin into its oligomers in deionized water.

Synergistically Promoted Antitumor Photoimmunotherapy Using Immune-Stimulating Peach Gum Polysaccharides as Nanocarriers.

Nanomaterial-based mild photothermal therapy (mPTT, 42-45 °C) with controllable light-triggered thermal diffusion holds potential in reversing immunosuppressive microenvironments and activating antitumor immunity in tumors. However, the limited antitumor efficacy of mPTT alone always requires sophisticated synergistic strategies to promote its overall therapeutic outcome. Herein, we employ an immune-active natural polymer, peach gum polysaccharide (PGP), as the nanocarrier to encapsulate the photothermal reagent of TTQPL to yield PLA-T nanoparticles (NPs).

Comparison of thoracoscopic-guided intercostal nerve block and ultrasound-guided intercostal nerve block in postoperative analgesia of uniportal video-assisted lobectomy: a pilot randomized controlled trial.

Background: Ultrasound-guided intercostal nerve block (UINB) and thoracoscopic-guided intercostal nerve block (TINB) are often used for analgesia after thoracic surgery. Herein, we compared the application of TINB and UINB for analgesia after uniportal video-assisted lobectomy.

Methods: Sixty patients were randomly allocated into two groups: UINB and TINB.

Bioinspired Polydopamine Modification for Interface Compatibility of PDMS-Based Responsive Structurally Colored Textiles.

Textiles that can repeatedly change color in the presence of external stimuli have attracted great interest. Effectively designing to produce such functional textiles is essential, yet there remain challenges like producing stable coloration, rapid response, and reverse color changing. Here, the preparation of a magnetic field response (MFR) textile with a fast magnetic field response, brilliant structural coloration, and mechanical robustness is reported.

Engineering High-Performance Composite Cellulose Materials for Fast Hemostasis.

The development of an effective hemostatic agents is of vital importance for saving wounded individuals from uncontrolled hemorrhage, which is the main reason for preventable death after accidental injury. However, current high-performance hemostatic agents suffer from a cumbersome preparation procedures and poor biocompatibility. Here, we engineered a cellulosic-derived aerogel material by simply controlling the drying process of cellulose regeneration for fast hemostasis.

The Effectiveness of Four Nicotinamide Adenine Dinucleotide (NAD) Precursors in Alleviating the High-Glucose-Induced Damage to Hepatocytes in : Evidence in NAD Homeostasis, Sirt1/3 Activation, Redox Defense, Inflammatory Response, Apoptosis, and Glucose Metabolism.

The administration of NAD precursors is a potential approach to protect against liver damage and metabolic dysfunction. However, the effectiveness of different NAD precursors in alleviating metabolic disorders is still poorly elucidated. The current study was performed to compare the effectiveness of four different NAD precursors, including nicotinic acid (NA), niacinamide (NAM), nicotinamide riboside (NR), and nicotinamide mononucleotide (NMN) in alleviating high-glucose-induced injury to hepatocytes in a fish model, .

Uncoordinated 51-like kinase 1a/b and 2 in fish Megalobrama amblycephala: Molecular cloning, functional characterization, and their potential roles in glucose metabolism.

Uncoordinated (Unc) 51-like kinase (ulk1) and ulk2 are closely involved in autophagy activation, but little is known about their roles in regulating glucose homeostasis. In this study, the genes of ulk1a, ulk1b and ulk2 were cloned and characterized in fish Megalobrama amblycephala. All the three genes shared the approximate N-terminal kinase domain and the C-terminal Atg1-like_tMIT domain structure, while the amino acid sequence identity of them are different between M.

Research on the village layout optimization in China's developed areas based on daily life circles.

Public service facilities are the basic material carrier of social services. It is of great significance for the operation of social justice and the improvement of residents' wellbeing. In the process of rapid urbanization, the demand for traffic convenience and diversity of public service facilities in rural areas has been further improved.

Genetic model of selective COX2 inhibition improve learning and memory ability and brain pathological changes in 5xFAD mouse.

Alzheimer's disease (AD) is the most common neurodegenerative disease that leads to dementia. Its pathogenesis is very complex, and inflammation is one of the main pathophysiological mechanisms of AD. Non-steroidal anti-inflammatory drugs (NSAIDs), which mainly target cyclooxygenase (COX) activity, are used to reduce the risk of AD, but several side effects limit their application.

Diagnosis of covert coarctation of the aorta in adolescents.

Objectives: By reviewing the diagnostic process for adolescents with coarctation of the aorta (CoA) in our institution, we analyzed the reasons for delayed diagnosis of CoA. We also proposed a diagnostic protocol to improve the detection rate of CoA.

Methods: In this retrospective study, we included 48 patients aged 12-18 years who were diagnosed with CoA in our hospital from January 2000 to November 2022.

Identification of circRNA-miRNA-mRNA regulatory network and its role in cardiac hypertrophy.

Background: Hypertrophic cardiomyopathy (HCM) is a grave hazard to human health. Circular RNA (circRNAs) and micro RNA (miRNAs), which are competitive endogenous RNA, have been shown to play a critical role inHCM pathogenicity. However, to a great extent, the biological activities of ceRNA in HCM pathophysiology and prognosis remain to be investigated.

Recent Advances in the Enzyme-Activatable Organic Fluorescent Probes for Tumor Imaging and Therapy.

The exploration of advanced probes for cancer diagnosis and treatment is of high importance in fundamental research and clinical practice. In comparison with the traditional "always-on" probes, the emerging activatable probes enjoy advantages in promoted accuracy for tumor theranostics by specifically releasing or activating fluorophores at the targeting sites. The main designing principle for these probes is to incorporate responsive groups that can specifically react with the biomarkers (e.

Deletion of 11q24.2-qter in a male child with cleft lip and palate: an atypical feature of Jacobsen syndrome.

Jacobsen syndrome (JS) is caused by the terminal deletion at the long arm of chromosome 11. It is characterized by growth retardation, intellectual disability, facial dysmorphism, and other congenital abnormalities. The subband 11q24.

PACT promotes the metastasis of basal-like breast cancer through Rac1 SUMOylation and activation.

Most basal-like breast cancers (BLBCs) are triple-negative breast cancers (TNBCs), which is associated with high malignancy, high rate of recurrence and distant metastasis, and poor prognosis among all types of breast cancer. However, there are currently no effective therapies for BLBC. Furthermore, chemoresistance limits the therapeutic options for BLBC treatment.

Exploring the trend in religious diversity: Based on the geographical perspective.

The formation and development of religious diversity is a manifestation of the free expression of human thought, belief, and practice, as well as a historical premise and ideological condition for the gradual recognition and integration of modern religions into modern political values. This study examines the spatial characteristics of the development of the global religious diversity index (RDI) and the evolution trend through a geographical perspective by the LISA space-time transition and convergence test. The results show that: (1) At the temporal level, RDI showed a fast and then slow increase after WWII, with an increase of 61.

Altered pain sensitivity in 5×familial Alzheimer disease mice is associated with dendritic spine loss in anterior cingulate cortex pyramidal neurons.

Chronic pain is highly prevalent. Individuals with cognitive disorders such as Alzheimer disease are a susceptible population in which pain is frequently difficult to diagnosis. It is still unclear whether the pathological changes in patients with Alzheimer disease will affect pain processing.

Suppressing NK Cells by Astragaloside IV Protects Against Acute Ischemic Stroke in Mice Inhibiting STAT3.

Natural killer (NK) cells, a key member of innate lymphocytes, are a promising immunotherapeutic target for ischemic stroke. Astragaloside IV (ASIV) is isolated from (Fabaceae), a herbal medicine possessing immunomodulatory ability. This study investigated the effect of ASIV on NK cells during the acute stage of brain ischemic injury in a mouse model of middle cerebral artery occlusion (MCAO).

Case Report: Three-Dimensional Printing-Assisted Surgical Treatment of Complex Body Vein Ectopic Drainage.

Introduction: Complex ectopic drainage of body veins is a rare congenital disease. Its preoperative diagnosis and surgical choice can be considerable challenges.

Case Summary: A 5-year-old patient was diagnosed precisely by preoperative transthoracic echocardiography, computed tomography (CT), three-dimensional (3D) reconstruction, and three-dimensional (3D) printing of the heart and great blood vessels.

Case Report: Transcatheter Closure of Ruptured Sinus of Valsalva With Ventricular Septal Defect Occluder in a 3-Year-Old Child After Repair of Ventricular Septal Defect.

Sinus of Valsalva aneurysm (SVA) is a rare cardiac anomaly that can undergo spontaneous rupture into other cardiac chambers or the pericardial space. A ruptured SVA has a very poor prognosis with high morbidity and mortality. These aneurysms often present as a rupture from the right coronary sinus into the right ventricle.

Tanshinone IIA alleviates NLRP3 inflammasome-mediated pyroptosis in Mycobacterium tuberculosis-(H37Ra-) infected macrophages by inhibiting endoplasmic reticulum stress.

Ethnopharmacological Relevance: Tanshinone IIA (Tan), extracted from Salvia miltiorrhiza Bunge, is a perennial herbal plant widely used as a folk remedy in Asian countries. Several studies have proved that Tanshinone IIA possesses many biological activities, such as anti-inflammatory, free-radical scavenging abilities, antioxidant properties, liver protection, and anti-cancer properties.

Aim Of The Study: The objective of the present study was to examine the anti-inflammatory effects of Tan.

A Rigid-Flexible Protecting Film with Surface Pits Structure for Dendrite-Free and High-Performance Lithium Metal Anode.

An artificial organic/inorganic composite protecting film for lithium metal anode with one-side surface pits structure was prepared by poly(vinylidene fluoride--hexafluoropropylene) and AlO+LiNO inorganic additives. Due to the unique surface structure, the composite film can not only serve as an artificial protective film, but also act as an additional lithium plating host, which synergistically enabled the lithium metal anode to adapt to high current densities meanwhile maintain dendrite-free during long-term cycling. As a result, the protected lithium metal anode can operate stably for 1000 h at a high current density of 10.

Magnesium Ion Storage Properties in a Layered (NH)VO·1.5HO Nanobelt Cathode Material Activated by Lattice Water.

Magnesium ion batteries have attracted increasing attention as a promising energy storage device due to the high safety, high volumetric capacity, and low cost of Mg. However, the strong Coulombic interactions between Mg ions and cathode materials seriously hinder the electrochemical performance of the batteries. To seek a promising cathode material for magnesium ion batteries, in this work, (NH)VO·1.

Study on the influencing factors of natural pectin's flocculation: Their sources, modification, and optimization.

Natural pectin, derived from fruit residue, presented a certain flocculation performance in previous studies. However, the process of extraction and treatment affected the flocculation performance considering the uncontrollable chemical composition and the molecule structure. In this study, degree of esterification (DE), degree of amidation (DA) were used as the internal factors affecting flocculation performance.