Identifying representative sequences of protein families using submodular optimization.

Identifying representative sequences for groups of functionally similar proteins and enzymes poses significant computational challenges. In this study, we applied submodular optimization, a method effective in data summarization, to select representative sequences for thioesterase enzyme families. We introduced and validated two algorithms, Greedy and Bidirectional Greedy, using curated protein sequence data from the ThYme (Thioester-active enzYmes) database.

CeO -anchored β-Ni(OH) nanosheets onto nickel foam for efficient energy-saving hydrogen production an electrocatalytic glucose oxidation reaction.

Electrolytic glucose oxidation has garnered great interest in energy-saving hydrogen generation. However, high charge-transfer resistance and inefficient active centers have been recognized as the primary issues for poor electrochemical performance. In this study, for the first time, we offer a novel defect-rich CeO /β-Ni(OH) composite nanosheet-decorated Ni foam electrocatalyst (denoted as Ce@NF-GA), synthesized a unique hydrothermal approach under the co-participation of glycerol and acetic acid.

Ethanolamine-induced assembly of microcompartments is required for virulence.

Unlabelled: Many bacteria metabolize ethanolamine as a nutrient source through cytoplasmic organelles named bacterial microcompartments (BMCs). Here we investigated the molecular assembly, regulation, and function of BMCs in a Gram-negative oral pathobiont that is associated with adverse pregnancy outcomes. The genome harbors a conserved ethanolamine utilization () locus with 21 genes that encode several putative BMC shell proteins and a two-component signal transduction system (TCS), in addition to the enzymes for ethanolamine transport and catabolism.

Ethanolamine-induced assembly of microcompartments is required for virulence.

Many bacteria metabolize ethanolamine as a nutrient source through cytoplasmic organelles named bacterial microcompartments (BMCs). Here we investigated the molecular assembly, regulation, and function of BMCs in - a Gram-negative oral pathobiont that is associated with adverse pregnancy outcomes. The genome harbors a conserved ethanolamine utilization () locus with 21 genes that encode several putative BMC shell proteins and a two-component signal transduction system (TCS), in addition to the enzymes for ethanolamine transport and catabolism.

The Orbitofrontal Cortex Is Required for Learned Modulation of Innate Olfactory Behavior.

Animals have evolved innate responses to cues including social, food, and predator odors. In the natural environment, animals are faced with choices that involve balancing risk and reward where innate significance may be at odds with internal need. The ability to update the value of a cue through learning is essential for navigating changing and uncertain environments.

Addressing Challenges of Membrane Clogging in AF4-UV-ICPMS Analysis for Size Determination of Trace Elements in Acidic, Organic-Rich Peat Bog Waters.

The analysis of colloid-associated trace elements (TEs) in acidic, organic-rich waters (pH 3.8-5.8) using AF4-UV-ICPMS often necessitates the use of neutral or weakly alkaline carriers (pH 7-8.

Impact of Cryopreserved Placental Allografts on Biochemical Recurrence in Prostate Cancer.

Background: Human placental allografts are widely used to promote wound healing. Placental (or amniotic membrane/umbilical cord) allografts are placed along the neurovascular bundles during radical prostatectomy to improve continence and erectile function recovery. It is unknown whether placental allografts impact biochemical recurrence (BCR).

A Comprehensive Protocol for the Collection, Differentiation, Cryopreservation, and Resuscitation of Primary Murine Bone Marrow Derived Macrophages (BMDM).

Background: The field of immunology has undoubtedly benefited from the use of cell lines for immunological studies; however, due to the "immortal" nature of many cell lines, they are not always the best model. Thus, direct collection and culture of primary cells from model organisms is a solution that many researchers utilize. To the best of our knowledge, there is not a singular protocol which encompasses the entire process of bone marrow cell collection through cryopreservation and resuscitation of cells from a murine model.

Investigating bottom phase extraction from aqueous two-phase systems for detecting bacteria using the lateral-flow immunoassay.

Lateral-flow immunoassays (LFAs) can be used to diagnose urinary tract infections caused by Escherichia coli (E. coli) at the point of care. Unfortunately, urine samples containing dilute concentrations of E.

Samae Dam chicken: a variety of the Pradu Hang Dam breed revealed from microsatellite genotyping data.

Objective: The remarkable adaptability to the environment, high growth rate, meat with good taste and aroma, and ornamental appearance of the Pradu Hang Dam (PDH) and Samae Dam (SD) chickens make them valuable for improvement of poultry production to enhance food security. However, despite their close phenotypic similarity, distinct classification of PDH and SD chickens remains controversial. Thus, this study aimed to clarify genetic origins and variation between PDH and SD chickens, genetic diversity and structures of PDH and SD chickens.

Health Literacy Analytics of Accessible Patient Resources in Cardiovascular Medicine: What are Patients Wanting to Know?

Introduction: There remains an increasing utilization of internet-based resources as a first line of medical knowledge. Among patients with cardiovascular disease, these resources often are relied upon for numerous diagnostic and therapeutic modalities. However, the reliability of this information is not fully understood.

Acceptance and User Experiences of a Wearable Device for the Management of Hospitalized Patients in COVID-19-Designated Wards in Ho Chi Minh City, Vietnam: Action Learning Project.

Background: Wearable devices have been used extensively both inside and outside of the hospital setting. During the COVID-19 pandemic, in some contexts, there was an increased need to remotely monitor pulse and saturated oxygen for patients due to the lack of staff and bedside monitors.

Objective: A prototype of a remote monitoring system using wearable pulse oximeter devices was implemented at the Hospital for Tropical Diseases in Ho Chi Minh City, Vietnam, from August to December 2021.

Evidence for conflict monitoring during speech recognition in noise.

During difficult tasks, conflict can benefit performance on a subsequent trial. One theory for such performance adjustments is that people monitor for conflict and reactively engage cognitive control. This hypothesis has been challenged because tasks that control for associative learning do not show such "cognitive control" effects.

A Novel Knee Traction Technique to Treat Chronic Knee Pain.

Chronic knee pain is one of the most common complaints in primary care offices. Osteopathic family physicians Leonid Tafler and Samira Ovshaev have worked tirelessly for over 10 years to develop a revolutionary and unique technique for treating chronic knee pain. This technique is easy to learn and can be performed at any outpatient practice.

Impact of a passive clinical decision support tool on potentially inappropriate medications (PIM) use in older adult patients.

Background: Medication related clinical decision support (CDS) interventions may improve patient safety. In older patient populations, there has been effort placed in reducing exposure to potentially inappropriate medications (PIMs). After years of reducing exposure of older adults in our hospitals to PIMs through multi-component interventions, our system chose to expand the scope and attempt a new strategy to lessen alert burden for providers and pharmacists.

Treatment characteristics and outcomes of pure Acinar cell carcinoma of the pancreas - A multicentric European study on radically resected patients.

Background: Acinar cell carcinomas (ACC) belong to the exocrine pancreatic malignancies. Due to their rarity, there is no consensus regarding treatment strategies for resectable ACC.

Methods: This is a retrospective multicentric study of radically resected pure pancreatic ACC.

A Workout to Remember: CrossFit-Induced Vertebral Artery Dissection.

Vertebral artery dissection (VAD) is a cause of brain stem stroke in the younger population and is commonly associated with trauma, sudden neck movement, or chiropractic manipulations. In this case, a 28-year-old male with a non-significant past medical history who recently started a CrossFit boot camp presented to the emergency department with right-sided neck pain, dysarthria, mild right gaze paresis, right dysmetria, and right facial droop with symptoms of Horner's syndrome. Imaging results revealed an age-indeterminate left lateral cerebellar infarct with right VAD.

Pancreatic Apoplexy: Fulminant Necrotizing Pancreatitis Leading to Completion Pancreatectomy Within 3 Days After Partial Pancreaticoduodenectomy.

Objectives: Patient characteristics with postoperative acute necrotizing pancreatitis and completion pancreatectomy (CP) after pancreaticoduodenectomy (PD) remain unclear.

Methods: Data from all patients who underwent a PD with need for CP (January 2011-December 2019) at a German University Hospital were analyzed regarding the indications and timing of CP, laboratory and histopathological findings, and overall outcome.

Results: Six hundred twelve patients underwent PD, 33 (5.

Applicability of the surgical risk calculator by the American College of Surgeons in the setting of German patients undergoing complete pancreatectomy: multicentre study using data from the StuDoQ|Pancreas registry.

Introduction: Surgical risk calculators can estimate risk probabilities for postoperative outcomes utilizing patient-specific risk factors. They provide meaningful information for obtaining informed consent. The aim of the present paper was to evaluate the predictive value of the surgical risk calculators by the American College of Surgeons in German patients undergoing total pancreatectomy.

Antibiotic perceptions, adherence, and disposal practices among parents of pediatric patients.

Antibiotics are frequently prescribed for children in the outpatient setting. Although sometimes necessary, antibiotic use is associated with important downstream effects including the development of antimicrobial resistance among human and environmental microorganisms. Current outpatient stewardship efforts focus on guiding appropriate antibiotic prescribing practices among providers, but little is known about parents' understanding of antibiotics and appropriate disposal of leftover antibiotics.