Thrombodynamics, a new global coagulation test: Measurement of heparin efficiency.

The actual coagulation status may be reliably measured using only highly sensitive global functional tests; however, they are not numerous and all of them have disadvantages. Thrombodynamics (TD), a novel global coagulation test, is sensitive to hypo- and hypercoagulable states. The main properties of this test were investigated, and its capabilities for hemostasis analysis were verified through pharmacodynamic monitoring of the most widely used anticoagulants, heparins.

Effect of Annexin V on Development of Apoptosis in Placental Syncytiotrophoblast Nuclei during Exacerbation of Cytomegalovirus Infection in the Third Trimester.

During exacerbation of cytomegalovirus infection during pregnancy, cytomegalovirus proteins penetrate across the external membrane of placental villous syncytiotrophoblast and suppress activities of ATPase and Bcl-2 protein. Simultaneous increase in the content of annexin V and caspase 3 leads to structural disturbances in DNA in syncytiotrophoblast nuclei and stimulates their apoptosis. Structural abnormalities in the syncytiotrophoblast during exacerbation of cytomegalovirus infection are dangerous for fetal development.

A Method for Detection of Cholesterol Synthesis in the Placenta during Exacerbation of Cytomegalovirus Infection.

Histochemical analysis showed that exacerbation of cytomegalovirus infection even at early terms of gestation (weeks 5-6) suppresses synthesis of mevalonic acid, squalene, and 7α-dehydrocholesterol dehydrogenase in the placenta. Inhibition of cholesterol biosynthesis in the placenta at the early stages of gestation decreases production of sex hormones, reduces cholesterol concentration in the maternal peripheral blood, and impairs cholesterol supply to the fetus, which is associated with the risk of placental insufficiency.

[Mechanisms of hypoxia development during pregnancy and the disorder of fetus blood supply at cytomegalovirus infection].

Objective: Our aim was to study the mechanisms of hypoxia development at pregnancy associated with cytomegalovirus infection (CMVI).

Methods: 30 parturient women with CMVI relapse at the 25-28 weeks of pregnancy and their newborns were examined. Cytochrome C, Hsp-70, p53, Bcl-2 and caspase-3 in placenta homogenate were found out with serologic methods, the morphology of erythrocytes with cytophotometry, erythrocytes membrane proteins with disc-electrophoresis method, TBA-active products with V.

[Transamination In Syncytiotrophoblast of Placenta Villi in Parturient Women Suffered the Acute Form of CMV Infection at the Third Trimester of Gestation].

Aim: to study the process of proteins transamination in syncytiotrophoblast of placenta villi of women who suffered the acute form of cytomega- lovirus (CMV) infection during pregnancy.

Methods: 30 pregnant women with CMV infection recurrence at the 25-28th week of pregnancy were examined. The activity of γ-glutamyltransferase in the peripheral blood of pregnant women was determined by spectrophotometry at the device "Stat-Fax-2100" (The USA).

Activity of histidine in peripheral blood erythrocytes of pregnant women during exacerbation of cytomegalovirus infection.

We studied the effect of active cytomegalovirus infection on histidine content in peripheral blood erythrocytes of pregnant women at gestation weeks 20-22 and its involvement into hemoglobin oxygenation. Using the histochemical technique developed by us, we studied the distribution of products of specific reaction for histidine in peripheral blood erythrocytes of pregnant women. The percentage of histidine-positive erythrocytes and their area were evaluated.

Morphofunctional characteristics of the glutathione cycle in peripheral blood erythrocytes of pregnant women with a history of cytomegalovirus infection exacerbation during gestation.

We studied factors influencing peripheral blood erythrocytes of pregnant women with exacerbation of cytomegalovirus infection during gestation. Cytomegalovirus infection was associated with a decrease in the content of reduced glutathione in erythrocytes and glutathione peroxidase, which leads to H2O2 accumulation in the blood. Exacerbation of cytomegalovirus infection was associated with reduction of glutathione reductase and SOD activities.

Enzymatic activity of peripheral blood erythrocytes in pregnant women with exacerbation of herpesvirus infection.

Enzymatic activity of the peripheral blood erythrocytes was studied in pregnant women after exacerbation of herpesvirus infection during the third trimester. Herpetic infection suppressed ATP synthesis and reduced the activities of glutathione reductase and glutathione peroxidase in erythrocytes. These shifts were paralleled by lesser intensity of SOD reaction, normally suppressing LPO processes in erythrocyte membranes.

Serotonin metabolism in the placenta in exacerbation of herpesvirus infection during pregnancy.

Serotonin metabolism in the blood and placental homogenate was studied in pregnancy aggravated by exacerbation of herpesvirus infection. Blood serotonin content in pregnant patients increased with increasing herpesvirus antibody titers and reduction of monoamine oxidase activity. Urinary excretion of 5-hydroxyindoleacetic acid (5-HIAA) decreased.

Morphology and functions of erythrocyte membranes in pregnant women after exacerbation of herpesvirus infection.

Herpesvirus infection is associated with an increase of LPO and of the membrane lipid and lipid-protein layers microviscosity in erythrocyte membranes of pregnant patients. This results in modification of the erythrocyte stromal protein composition. Erythrocyte deformability increases sharply under these conditions, this leading to disorders in tissue metabolism in the organs of pregnant patients.

[Oxygen-transport function of blood at pregnant, transferred the aggravation of the herpesvirus infection].

Factors which may influence on erythrocytes in peripheral blood while exacerbation of herpes infection are studied. The data showed that herpes infection changed quantitative spectrum of proteins in erythrocytes. In cellular membranes of erythrocytes lipid peroxidation increases that results in increase of microviscosity of membranes.

[DHEA-s synthesis during pregnancy with cytomegalovirus infection].

Aggravation of cytomegalovirus infection during pregnancy suppresses synthesis of DHEA-s and damages the adrenal glands and liver of fetus.

[Minimally invasive methods for the treatment of the recurrent varicosis].

The experience of 193 cases of recurrent variceal disease (RVD) was summarized. Major reasons of recurrences after surgery were analyzed. Traditional and miniinvasive RVD treatment approaches are listed and its efficacy was thoroughly analyzed.

Morphofunctional characteristics of fetoplacental barrier of placental villi during pregnancy complicated by herpes-virus infection.

Analysis of placenta homogenate from pregnant women having an episode of herpes-virus infection during the third trimester revealed suppression of Bcl-2 protein activity and increase in caspase 3 concentration. Activity of Bcl-2 protein decreases with increasing the titer of antibodies to herpes virus. Increased caspase-3 activity increases the risk of DNA damage in syncytiotrophoblast nuclei.

Morphofunctional characteristics of syncytiotrophoblast and content of heat shock protein 70 in it during exacerbation of herpesvirus infection in pregnant women.

Exacerbation of herpesvirus infection during pregnancy is associated with damage to syncytiotrophoblast inflicted by herpesvirus either directly or via TNF-α (due to contact of NK lymphocytes with villus surface). A sharp decrease in the content of heat shock protein with a molecular weight of 70 Da and activation of caspase-3 were noted in placenta homogenate. This leads to disturbances in syncytiotrophoblast cytosol structure and increase in the relative content of apoptotic nuclei.

[Morphofunctional characteristics of the fetoplacental complex in pregnant women with an exacerbation of herpes virus infection and pathomorphological changes in fetal organs].

Impaired placental cholesterol synthesis in a pregnant woman with an exacerbation of herpesvirus infection is characterized by disturbances in placental hormone biosynthesis, causing severe morphological changes in fetal organs (liver, adrenals, lungs, and thymus).

[Pathogenesis of mucociliary clearance].

The study of mucociliary clearance included 68 patients with bronchial asthma of different severity in whom viscoelastic properties of the sputum, motor activity of ciliary epithelium, glycosaminoglycans in peripheral blood and bronchial secretion at the B9-B10 level of the bronchial tree were determined. Simultaneously, morphofunctional state of bronchial mucosa was evaluated using DNA methylation-specific PCR.

[Computer method for the assessment of epithelial metaplasia in human bronchial mucous membrane].

Using Bio Vision computer program, the successive stages of metaplastic changes development were traced in the pseudostratified columnar ciliated epithelium of bronchial mucous membrane obtained from 65 patients with bronchial asthma of various degrees of severity. Biopsy material analysis demonstrated the changes of cell nuclear and cytoplasmic density, as well as of basement membrane.

[Endovascular laser ablation with wavelength 1,560 nm for varicose veins].

In 2002 - 2008 more than 1000 patients with lower limb varicose veins were treated with endovenous laser coagulation (EVLC) of subcutaneous and perforating veins in the Therapeutic Rehabilitation Center. Usually we used laser wavelength between 915 and 980 nm; but recently 1,560-nm laser, which physical properties are quite different, was used for 43 patients, including 49 EVLCs for great (GSV) and lesser (LSV) saphenous veins and 15 EVLCs for perforating veins (PV). Interventional technique was similar to previously used.

[Computer assessment of an apoptotic process dynamics in the nuclei of placental villi syncytiotrophoblast in puerperas after the outburst of herpes virus infection].

The development of an apoptotic process in the nuclei of syncytiotrophoblast was studied in the placenta of 50 puerperas, who had the outburst of herpes virus infection in the second part of their pregnancy (antibody titre--1:12800). Control group was represented by the placental material obtained from 20 women with no disease. Apoptosis was demonstrated in paraffin sections of the material fixed in 10% buffered formalin by in situ labeling of DNA fragments (ISEL-method).

[Computer assessment of membrane structure in various erythrocyte forms].

The photometric computer image analysis method is described. It is based on the creation of the gallery of the images of various erythrocyte forms (discocytes, ecchinocytes, target cells and degenerative forms). Using the Bio Vision program, the structure of membranes of each type of erythrocytes was studied.

[The immunomorphological characteristics of collagen in the mucosa of human bronchi].

By means of immunomorphological assay the character of different collagen types distribution in human bronchial mucosa has been investigated. It has been established that collagen tends to have a marked topographic specificity of distribution in human bronchial mucosa. Collagens of the I and the III types are mostly distributed in basal membranes of mucous glands' terminal parts.

[Type II alveolocyte--a subsidiary cell of the pulmonary immune response].

Distribution of secretory immunoglobulin A has been studied by means of a direct and indirect immunohistochemical reactions in semithin and ultrathin slices, using operative and biopsy material of the human respiratory part of the lungs. The data obtained prove existence of 2 types of cells, which possess properties of subsidiary immune cells. They include alveolar macrophages and alveolocytes of II type.

[Immunomorphological determination of secretory immunoglobulin A in the bronchial mucous membrane in chronic bronchitis].

The disappearance of the secretory immunoglobulin A in chronic bronchitis is shown to result from the disappearance of the epitheliocytes capable of secreting this substance. The secondary immunoglobulin A deficiency is followed by its accumulation in the basal epithelial cells. The absence of the secretory component probably prevents the immunoglobulin A diffusion to the epithelium surface this decreasing the local immunity against various antigens and the possibility of their elimination.