An acid-free sensing strategy for detecting nitrite using dihydroquinoline-8-carboxylate as a probe.

Nitrite (NO) has been identified as a typical pollutant harmful to the human body and heavily assayed in the fields of food safety and water quality control. The mainstream sensing strategies for detecting NO depend on Griess reaction or its improved methods which employ Griess reaction to initiate further inter-or intramolecular interaction to generate readout signals. However, a significant drawback of these methods is the use of strongly acidic media.

A polystyrene-based ESIPT fluorescent polymeric probe for highly sensitive detection of chromium(vi) ions and protein staining.

A "two-step" preparation method of an excited-state intermolecular proton transfer (ESIPT) fluorescent polymer (f-PP) is reported here. The synthesis of f-PP involves the acetylation of polystyrene and a "multicomponent one pot" reaction. The as-prepared polymer bears a group of ESIPT fluorescent units, enabling it to exhibit high brightness, moderate solubility and ESIPT fluorescence.

A fluorescent probe based on modulation of ESIPT signaling for the highly selective detection of NH and cell-imaging.

The broad occurrence of the hydrazine (NH) residues in aqueousenvironment is a potential threat to human health. Currently, the mainstream strategy for designing NH-specific probes is to functionalize a fluorophore with nucleophilic sites for the reductionreaction with NH. In this work, we designed and synthesized an excited-state intermolecular proton transfer (inter-ESPT) fluorescent dye(2-amino-4-(4-methoxyphenyl)-7,8-dihydro-5H-spiro[quinoline-6,2'-[1,3]dioxolane]-3-carbonitrilem, DQN) and used it as a probe to sense NH.

Protein-activated and FRET enhanced excited-state intermolecular proton transfer fluorescent probes for high-resolution imaging of cilia and tunneling nanotubes in live cells.

Excited-state intermolecular proton transfer (inter-ESPT) fluorescent probes responsive to specific bioactive molecules should be greatly promising for protein sensing, DNA mutation simulating and cellular process regulating. However, the inter-ESPT molecules are recessive ESPT fluorophores, which need the assistance of other molecules with both hydrogen-bond accepting and donating abilities to turn on the tautomeric fluorescence. Valid design strategies to create powerful inter-ESPT fluorescent probes are poorly developed, particularly for proteins as targets.

Spectral Properties Echoing the Tautomerism of Milrinone and Its Application to Fe Ion Sensing and Protein Staining.

Knowledge on the spectral properties of the tautomers of milrinone (MLR) in solvents and solid-state, as well as under light conditions is of critical importance from both theoretical and practical points of view. Herein, we investigated the spectral properties of MLR in different conditions using UV-Vis and fluorescence spectroscopies. The experimental results demonstrated that MLR can undergo the tautomerization reaction induced by solvent polarity, light and pH, eliciting four tautomeric structures (enol, keto, anion, and cation forms).