Preparation of Bispecific IgY-scFvs Inhibition Adherences of Enterotoxigenic (K88 and F18) to Porcine IPEC-J2 Cell.

Enterotoxigenic (ETEC) strains are significant contributors to postweaning diarrhea in piglets. Of the ETEC causing diarrhea, K88 and F18 accounted for 92.7%.

Simultaneous detection of glycinin and β-conglycinin in processed soybean products by high-performance liquid chromatography-tandem mass spectrometry with stable isotope-labeled standard peptides.

Glycinin and β-conglycinin are the two main allergic proteins in soybean. Due to their complex structures and lack of protein standards, it is difficult to achieve quantitative determination of these proteins in soybeans. In this study, an HPLC-MS/MS method was developed for the simultaneous determination of five subunits of glycinin (G1, G2, G3, G4, and G5) and three subunits of β-conglycinin (α, α', and β) in processed soybean products based on 8 specific peptides and their stable isotope-labeled peptides.

Blocked conversion of Lactobacillus johnsonii derived acetate to butyrate mediates copper-induced epithelial barrier damage in a pig model.

Background: High-copper diets have been widely used to promote growth performance of pigs, but excess copper supplementation can also produce negative effects on ecosystem stability and organism health. High-copper supplementation can damage the intestinal barrier and disturb the gut microbiome community. However, the specific relationship between high-copper-induced intestinal damage and gut microbiota or its metabolites is unclear.

Dual quantum dot nanobeads-based fluorescence-linked immunosorbent assay for simultaneous detection of aflatoxin B1 and zearalenone in feedstuffs.

A novel dual quantum dot nanobeads-based fluorescence-linked immunosorbent assay (QBs-FLISA) was successfully developed for simultaneously detecting aflatoxin B1 (AFB1) and zearalenone (ZEN) in feedstuffs. Dual CdSe/ZnS quantum dot nanobeads with different diameters that emit red and green fluorescence were conjugated with anti-AFB1 and anti-ZEN monoclonal antibodies to prepare fluorescent probes, which greatly enhance analytical performance. Under the optimal conditions, the limits of detection for AFB1 and ZEN were 9.

Excellent Carbonation Behavior of Rankinite Prepared by Calcining the C-S-H: Potential Recycling of Waste Concrete Powders for Prefabricated Building Products.

Pure rankinite (C₃S₂) was prepared by calcining a C-S-H gel precursor at a temperature of 1300 °C. The carbonation hardening behavior of the resulting rankinite was revealed by X-ray diffraction (XRD), Fourier transform-infrared (FT-IR) spectroscopy, thermogravimetry and differential thermal analysis (TG/DTA), and scanning electron microscope (SEM) coupled with energy dispersive spectrum (EDS). The results indicate that the pure rankinite can be easily prepared at a lower temperature.

Metal pollution in Huayuan River in Hunan Province in China by manganese sulphate waste residue.

The Huayuan River in Hunan Province in China is subject to ongoing mining activity with Mn extraction. In this study, the level and environmental significance of metals (including Mn, Cd, Pb, Cu, Zn, Ni and Fe) concentrations in the surface water and river sediments have been investigated along a 187 km reach of the Huayuan River. Using the X-ray fluorescence (XRF) analysis, we analyzed the characterization of metals in manganese sulphate waste residue (MSWR) deposited along the bank of Huayuan River.

[Effects of sinomenine on NO/nNOS system in cerebellum and spinal cord of morphine-dependent and withdrawal mice].

To explore the effect of sinomenine on the nitric oxide (NO)/neural nitric oxide synthase (nNOS) system in the cerebellum and spinal cord of morphine-dependent and morphine-withdrawal Kunming mice, mice were subjected to injection of morphine with an increasing dose for 5 d, and then were treated with sinomenine (40 mg/kg, i.p.) for another 5 d.