Genome-wide identification of FTZ-F1 genes in Chlamys farreri and analysis of ChIP-seq-based binding sites and potential target genes.

FTZ-F1 (Fushi tarazu factor-1) is a crucial member of the monomeric orphan nuclear receptor family, playing essential roles in reproductive development, steroidogenesis, and metabolism. However, studies on the function of FTZ-F1 and its target genes in bivalve mollusks remain limited. In this study, we conducted a genome-wide analysis of Chlamys farreri and identified two FTZ-F1 family members, designated as Cf FTZ-F1 and Cf FTZ-F1b.

The source-specific health risk and biological effect assessment of PAHs in Mactra veneriformis from the Bohai Sea and Yellow Sea.

Source-specific risk assessment has been widely used to analyze the source of pollution risk instead of pollutant content for more accurate control of pollution. However, due to limitations in data type, assessment of source-specific biological effect has not been reported, which is highly significant for organism protection. This study conducted a comprehensive assessment of the health risk and biological effect of polycyclic aromatic hydrocarbons (PAHs) bioaccumulated in the clam Mactra veneriformis from two typical bays in China.

Role of NR5A2 in regulating sex differentiation, steroidogenesis, and gonadal development in Chlamys farreri.

Chlamys farreri is a commercially important bivalve species in global aquaculture. However, research on the mechanisms regulating its sex differentiation and reproduction remains relatively sparse. In this study, the role of nuclear receptor subfamily 5 group A member 2 (NR5A2) in sex differentiation, steroidogenesis, and gonadal development in C.

A structural equation model for assessing toxic effects of polycyclic aromatic hydrocarbons on scallop Chlamys farreri and the establishment of a novel integrated biomarker index.

Persistent organic pollutants (POP) occur in coastal areas worldwide and seriously affect marine ecosystems, especially aquatic organisms. Here, we assessed the bioaccumulation, biological effects, and health risks of polycyclic aromatic hydrocarbons (PAHs), one typical kind of global POPs, in the scallop Chlamys farreri from typical bays of the Yellow Sea and the Bohai Sea, China. Results revealed higher PAH bioaccumulation in scallops from Laizhou Bay and Jiaozhou Bay compared to Rushan Bay and Haizhou Bay.

Study on the detoxification and toxic effects of clam Ruditapes philippinarum exposed to 2,6-ditert-butyl-4-methylphenol (BHT): Oxidative stress, neurotoxicity, respiratory toxicity, and immunotoxicity.

The bioaccumulation and toxic effects of synthetic phenolic antioxidants (SPAs) in aquatic ecosystems are of growing concern due to their widespread use and potential environmental persistence. This study investigated the detoxification and toxicological impacts of 2,6-ditert-butyl-4-methylphenol (BHT), a representative SPAs, on clams Ruditapes philippinarum using environmentally relevant exposure concentrations. BHT bioaccumulation was observed in both gills and digestive glands, with higher levels in the latter.

Mechanisms of intestinal DNA damage and inflammation induced by ammonia nitrogen exposure in Litopenaeus vannamei.

Ammonia nitrogen, a common aquaculture pollutant, harms crustaceans by causing intestinal inflammation, though its exact mechanisms are unclear. Thus, we exposed shrimp to 0, 2, 10 and 20 mg/L NHCl exposure for 0, 3, 6, 12, 24, 48, 72 h, and explored the intestinal stress, apoptosis, proliferation, inflammation and its histopathological changes. This research indicated that ammonia nitrogen exposure heightens plasma dopamine (DA), 5-hydroxytryptamine (5-HT), norepinephrine (NE), and acetylcholine (ACh) levels, alters gene expression of neurotransmitter receptors in the intestine, triggering the PLCCa pathway and induces endoplasmic reticulum stress.

Adaptation mechanism of clam Ruditapes philippinarum under polycyclic aromatic hydrocarbon stresses: Accumulation and excretion.

Persistent organic pollutants (POPs) are easily accumulated in organisms due to its high lipophilicity. Bivalves are still one of the marine invertebrates with high species diversity despite experiencing with environmental pollution. However, studies investigating the adaptation mechanisms of bivalves towards POPs have been lacking.

Studies on the characteristics of polycyclic aromatic hydrocarbons accumulation in lipids and the disturbance of lipid metabolism of Ruditapes philippinarum.

Polycyclic aromatic hydrocarbons (PAHs) constitute a class of persistent organic pollutants with strong lipophilicity, which readily accumulate within organisms and have the effect to induce disorders in lipid metabolism. The present study aimed to investigate the accumulation localization and pattern of PAHs in Ruditapes philippinarum, and to reveal the association between PAHs and lipids metabolism. The 21-day exposure experiment was conducted using a mixture of phenanthrene, chrysene, and benzo[a]pyrene (the proportion is 1:1:1) at concentrations of 0.

Estrogen receptor knockdown suggests its role in gonadal development regulation in Manila clam Ruditapes philippinarum.

The estrogen receptor (ER), a ligand-dependent transcription factor, is critical for vertebrate reproduction. However, its role in bivalves is not well understood, with ongoing debates regarding its function in regulating reproduction similarly to vertebrates. To investigate ER's function, we conducted a 21-day RNA interference experiment focusing on its role in gonadal development in bivalves.

Species sensitivity distribution for nonylphenol: Acute toxicity and ecological risk in Bohai Region.

Nonylphenol (NP), a main byproduct of nonylphenol polyethoxylates (NPEs) degradation, is prevalent across diverse environmental settings. Given its widespread presence, evaluating the ecological risks associated with NP in coastal waters and sediments is essential for the protection of the marine environment. This study evaluates the acute toxicity of NP on ten representative aquatic species from the Bohai Sea, determining the Aquatic Life Criteria (ALC) through two distinct methods.

Effects of dietary glycerol monolaurate on growth and digestive performance, lipid metabolism, immune defense and gut microbiota of shrimp (Penaeus vannamei).

The advancement of the Penaeus vannamei industry in a sustainable manner necessitates the creation of eco-friendly and exceptionally effective feed additives. To achieve this, 720 similarly-sized juvenile shrimp (0.88 ± 0.

The mechanism of reactive oxygen species generation, DNA damage and apoptosis in hemocytes of Litopenaeus vannamei under ammonia nitrogen exposure.

Ammonia-N poses a significant threat to aquatic animals. However, the mechanism of ROS production leading to DNA damage in hemocytes of crustaceans is still unclear. Additionally, the mechanism that cells respond to DNA damage by activating complex signaling networks has not been well studied.

Mechanisms of neurocentral-eyestalk-intestinal immunotoxicity in whiteleg shrimp Litopenaeus vannamei under ammonia nitrogen exposure.

Ammonia-N, as the most toxic nitrogenous waste, has high toxicity to marine animals. However, the interplay between ammonia-induced neuroendocrine toxicity and intestinal immune homeostasis has been largely overlooked. Here, a significant concordance of metabolome and transcriptome-based "cholinergic synapse" supports that plasma metabolites acetylcholine (ACh) plays an important role during NHCl exposure.

Exploring the mechanism of nonylphenol-induced ovarian developmental delay of manila clams, Ruditapes philippinarum: Applying RNAi to toxicological analysis.

Nonylphenol (NP) contamination in the coastal environment of China poses ecological risks to aquatic organisms. However, the endocrine disruptive impacts of NP on bivalves, particularly on ovarian development, remain poorly understood. In this study, Manila clams Ruditapes philippinarum at the developing stage of gonad were exposed to 1.

Occurrence, distribution, and health risk assessment of pyrethroid and neonicotinoid insecticides in aquatic products of China.

Synthetic pyrethroid insecticides (SPIs) and neonicotinoid insecticides (NEOs), now dominant in the insecticide market, are increasingly found in aquatic environments. This study focused on six SPIs and five NEOs in aquatic products from four Chinese provinces (Shandong, Hubei, Shanxi and Zhejiang) and the risk assessment of the safety for the residents was conducted. It revealed significantly higher residues of ΣSPIs (6.

Mechanisms of ammonotelism, epithelium damage, cellular apoptosis, and proliferation in gill of Litopenaeus vannamei under NHCl exposure.

Excessive ammonia-N in coastal environment and aquaculture threatens the health of marine organisms. To explore the mechanism of gill damage induced by ammonia-N, transcriptome of Litopenaeus vannamei 's gill was carried out under 20 mg/L NHCl for 0, 6, and 48 h. K-means clustering analysis suggested that ammonia excretion and metabolism-related genes were elevated.

Interference mechanism of benzo[a]pyrene exposure on the taste substance metabolisms in Ruditapes philippinarum.

Aquatic animals are popular for their unique umami and high-quality protein. However, under the realistic background of increasing marine pollution, whether it affects the aquatic animal tastes, and what the interference mechanism is still remains unknown. Benzo[a]pyrene (B[a]P) is a typical Polycyclic aromatic hydrocarbons (PAHs) with high toxicity.

Study on epigenotoxicity, sex hormone synthesis, and DNA damage of benzo[a]pyrene in the testis of male Ruditapes philippinarum.

Research on the mechanisms of reproductive toxicity caused by persistent organic pollutants (POPs) in marine animals has received significant attention. One group of typical POPs, called polycyclic aromatic hydrocarbons (PAHs), has been found to cause various reproductive toxicities in aquatic organisms, including epigenotoxicity, reproductive endocrine disruption, DNA damage effects and other reproductive toxicity, thereby affecting gonadal development. Interestingly, male aquatic animals are more susceptible to the disturbance and toxicity of environmental pollutants.

The assessment of bioavailability and environmental risk of dissolved and particulate polycyclic aromatic hydrocarbons in the seawater of typical bays.

The pollution of dissolved and particulate polycyclic aromatic hydrocarbons (PAHs) in coastal waters has been increasing in recent decades. However, limited research has been conducted on the characteristics of dissolved and particulate PAHs in seawater and their associated risk assessment. Here, we focused on the bioavailability and environmental risk of PAHs in four typical bays of Shandong Province, China, and used scallop Chlamys farreri and clam Mactra veneriformis as sentinel species.

Effects of Benzo[a]pyrene on Food Metabolism and Reproductive Endocrine and Ovarian Development in Female Scallop Chlamys farreri at Different Reproductive Stages.

Benzo[a]pyrene (B[a]P), a polycyclic aromatic hydrocarbon (PAH) with the most carcinogenic effects of all the PAHs, has multiple toxic effects on marine bivalves. We investigated the interference mechanism of B[a]P on food metabolism (sugars, proteins, and sugars), and on reproductive endocrine and ovarian development in female scallops (Chlamys farreri). Scallops were exposed to different concentrations of B[a]P concentrations of 0, 0.

Desulfurization properties, pathways, and potential applications of two novel and efficient chemolithotrophic sulfur-oxidizing strains of Pseudomonas sp. GHWS3 and Sphingobacterium sp. GHWS5.

With the accelerated modernization of agriculture and industry, sulfides have been released into the environment as a by-products of various production processes. Elevated levels of sulfide pose a threat to organisms' health and disrupt ecosystem equilibrium. This study successfully isolated two highly efficient sulfur-oxidizing strains, namely Pseudomonas aeruginosa GHWS3 and Sphingobacterium sp.

The immunotoxicity mechanism of hemocytes in Chlamys farreri incubated with noradrenaline and benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide alone or in combination.

Benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide (BPDE) is the active intermediate metabolite of benzo[a]pyrene (B[a]P) and is considered the ultimate immunotoxicant. The neuroendocrine immunoregulatory network of bivalves is affected under pollutant stress. Besides, bivalves are frequently affected by pollutants in marine environments, yet the combined effects of neuroendocrine factors and detoxification metabolites on bivalves under pollutant stress and the signal pathways that mediate this immunoregulation are not well understood.

Source-specific ecological and health risks of polycyclic aromatic hydrocarbons in the adjacent coastal area of the Yellow River Estuary, China.

Industrialization and urbanization have led to increasing levels of PAH pollution in highly urbanized estuaries and their adjacent coastal areas globally. This study focused on the adjacent coastal area of the Yellow River Estuary (YRE) and collected surface seawater, surface sediment, and clams and at four sites (S1 to S4) in May, August, and October 2021 to analyze the source-specific ecological and health risks and bioeffects. The findings revealed that the main sources of PAHs were traffic emission (25.

Study on reproductive endocrine disturbance and DNA damage mechanism of female Ruditapes philippinarum under Benzo[a]pyrene stress.

The reproductive toxicity of polycyclic aromatic hydrocarbons (PAHs) in aquatic organisms has attracted increasing attention from scholars. Currently, research in this field primarily focuses on vertebrates such as zebrafish and other model species. However, there is still a significant knowledge gap in the toxicity of PAHs to invertebrates and its potential mechanisms.