Light signal regulates endoreduplication and tomato fruit expansion through the SlPIF1a-SlTLFP8-SlCDKB2 module.

Light serves as an energy source for cell division and expansion during fruit development. Cell expansion significantly influences fruit size and is closely linked to endoreduplication, a unique cell cycle variation characterized by DNA replication without cytokinesis. Paradoxically, under conditions of ample photosynthates, light signaling suppresses cell expansion.

Integrating network pharmacology and model to reveal the cardiovascular protective effects of kaempferol-3--rutinoside on heart failure.

Kaempferol-3--rutinoside (KR) has an excellent cardioprotective effect, but its mechanism of action is not clear. Network pharmacology was used to predict the signaling pathways, whereas molecular docking was used for preliminary validation of KR binding to targets. AMI model rats with ligated left anterior descending coronary arteries were established.

RING finger ubiquitin E3 ligase CsCHYR1 targets CsATAF1 for degradation to modulate the drought stress response of cucumber through the ABA-dependent pathway.

CsCHYR1 (CHY ZINC-FINGER AND RING PROTEIN1) encodes a RING (Really Interesting New Gene) finger E3 ubiquitin ligase involved in ubiquitin-mediated protein degradation and plays an important role for cucumber to resist drought stress. Here, we obtain one of the candidate proteins CsCHYR1 that probably interacts with CsATAF1 by yeast-two hybrid screening. Subsequently, it is verified that CsCHYR1 interacts with CsATAF1 and has self-ubiquitination activity.

Heat shock-induced cold acclimation in cucumber through CsHSFA1d-activated JA biosynthesis and signaling.

Cucumber (Cucumis sativus) originated in tropical areas and is very sensitive to low temperatures. Cold acclimation is a universal strategy that improves plant resistance to cold stress. In this study, we report that heat shock induces cold acclimation in cucumber seedlings, via a process involving the heat-shock transcription factor HSFA1d.

Research Advances in Heterotrimeric G-Protein α Subunits and Uncanonical G-Protein Coupled Receptors in Plants.

As crucial signal transducers, G-proteins and G-protein-coupled receptors (GPCRs) have attracted increasing attention in the field of signal transduction. Research on G-proteins and GPCRs has mainly focused on animals, while research on plants is relatively rare. The mode of action of G-proteins is quite different from that in animals.

CRISPR/Cas9 targeted mutagenesis of SlLBD40, a lateral organ boundaries domain transcription factor, enhances drought tolerance in tomato.

The LATERAL ORGAN BOUNDARIES DOMAIN (LBD)-containing genes are plant-specific genes that play important roles in lateral organ development. In this study, we identified LBD40 (Solyc02g085910), which belongs to subfamily II of the LBD family of genes in tomato. LBD40 was highly expressed in roots and fruit.

SlTLFP8 reduces water loss to improve water-use efficiency by modulating cell size and stomatal density via endoreduplication.

Improving plant water-use efficiency (WUE) is important to plant survival and crop yield in the context of water limitation. In this study, SlTLFP8 (Tubby-like F-box protein 8) was identified as an osmotic-induced gene in tomato. Transgenic tomato with up-regulated expression of SlTLFP8 showed enhanced water-deficient resistance, whereas knockout mutants generated by CRISPR/Cas9 were more sensitive to water deficit.

Melatonin promotes carotenoid biosynthesis in an ethylene-dependent manner in tomato fruits.

In tomato, red color is a key commercial trait and arises from the accumulation of carotenoids. Previous studies have revealed that melatonin promotes lycopene accumulation and ethylene production. However, it is unclear if melatonin similarly increases other carotenoids, and whether any increase of carotenoids in tomato fruit is directly related to ethylene production.

Jasmonate and aluminum crosstalk in tomato: Identification and expression analysis of WRKYs and ALMTs during JA/Al-regulated root growth.

The WRKY transcription factors (TFs) are involved in aluminum (Al) stress and jasmonic acid (JA)-regulated resistance responses. WRKYs act as regulators of Al-activated malate transporter (ALMT) proteins (anion channels) by directly binding to their promoters and altering malate efflux, thereby regulating Al ion toxicity in plant roots. JA enhances Al-induced root growth inhibition in Arabidopsis.

In silico genome-wide identification and comprehensive characterization of the gene family in soybean.

The transcription factor family play a central role in brassinosteroid signaling pathway that regulates a wide range of plant growth and developmental processes, as well as resistances to various stresses. However, no comprehensive study of the gene family in soybean has been reported. In this work, 16 -like genes were identified in soybean, which could be divided into two clades based on their phylogenetic relationships, gene structures and motif compositions.

The transcription factor family in soybean: Genome-wide identification, expression profiling and genetic diversity analysis.

The transcription factor family plays a critical role in the ethylene signaling pathway, which regulates a broad spectrum of plant growth and developmental processes, as well as defenses to myriad stresses. Although genome-wide analysis of this family has been carried out for several plant species, no comprehensive analysis of the gene family in soybean has been reported so far. Furthermore, there are few studies on the functions of genes in soybean.

Genome wide characterization of simple sequence repeats in watermelon genome and their application in comparative mapping and genetic diversity analysis.

Background: Microsatellite markers are one of the most informative and versatile DNA-based markers used in plant genetic research, but their development has traditionally been difficult and costly. The whole genome sequencing with next-generation sequencing (NGS) technologies provides large amounts of sequence data to develop numerous microsatellite markers at whole genome scale. SSR markers have great advantage in cross-species comparisons and allow investigation of karyotype and genome evolution through highly efficient computation approaches such as in silico PCR.