Characterizing progenitor cells in developing and injured spinal cord: Insights from single-nucleus transcriptomics and lineage tracing.

Various mature tissue-resident cells exhibit progenitor characteristics following injury. However, the existence of endogenous stem cells with multiple lineage potentials in the adult spinal cord remains a compelling area of research. In this study, we present a cross-species investigation that extends from development to injury.

Simultaneous determination of twelve microcystins in algal supplements on the Chinese market and possible risks to exposed populations.

Algae supplements are widely recognized for their nutritional benefits and are commonly marketed as natural health products. However, concerns regarding contamination with cyanobacterial toxins have been raised. Moreover, there is very little data regarding the potential contamination of algal supplements on the Chinese market by these toxins.

Aberrant Cortical Morphological Networks in First-Episode Schizophrenia.

Background And Hypothesis: Population-based morphological covariance networks are widely reported to be altered in schizophrenia. Individualized morphological brain network approaches have emerged recently. We hypothesize that individualized morphological brain networks are disrupted in schizophrenia.

A Universal Strategy of Anti-Tumor mRNA Vaccine by Harnessing "Off-the-Shelf" Immunity.

Personalized neoantigen cancer mRNA vaccines are promising candidates for precision medicine. However, the difficulty of identifying neoantigens heavily hinders their broad applicability. This study developed a universal strategy of anti-tumor mRNA vaccine by harnessing "off-the-shelf" immunity to known antigens.

Status of a New Model of ERCP Training in China: A Nationwide Survey of Standardized Training for ERCP Procedure (STEP) Programs.

Aims: ERCP training should adapt to the rapid development of ERCP technology and indications. China has a large population and vast land area, but ERCP training resources are insufficient. To make effective use of scarce ERCP training resources, the Standardized Training for ERCP Procedure (STEP) program was launched as a pilot trial.

FAK inhibition delays liver repair after acetaminophen-induced acute liver injury by suppressing hepatocyte proliferation and macrophage recruitment.

Background: Overdose of acetaminophen (APAP), a commonly used antipyretic analgesic, can lead to severe liver injury and failure. Current treatments are only effective in the early stages of APAP-induced acute liver injury (ALI). Therefore, a detailed examination of the mechanisms involved in liver repair following APAP-induced ALI could provide valuable insights for clinical interventions.

ALG5 downregulation inhibits osteogenesis and promotes adipogenesis by regulating the N-glycosylation of SLC6A9 in osteoporosis.

Osteoporosis is characterized by decreased bone mass and accumulation of adipocytes in the bone marrow. The mechanism underlying the imbalance between osteoblastogenesis and adipogenesis in bone marrow mesenchymal stem cells (BMSCs) remains unclear. We found that ALG5 was significantly downregulated in BMSCs from osteoporotic specimens.

Dose effect of corticosteroids on peripheral lymphocyte profiles in patients with systemic lupus erythematosus.

Objective: To investigate the dose effect of methylprednisolone (MP) on peripheral lymphocyte profiles in patients with systemic lupus erythematosus (SLE). This study investigated the impact of varied MP doses on peripheral lymphocyte subtypes in SLE patients.

Methods: We conducted a prospective study involving 51 SLE patients, categorized into four groups (40 mg/day, 80 mg/day, 500 mg/day, and 1000 mg/day) based on the administered MP dosage during hospitalization.

Real-world effectiveness and safety of bleomycin in patients with keloids and hypertrophic scars: a systematic review and meta-analysis.

Pathological scars are classified into hypertrophic scars and keloids, and currently have poor treatment outcomes and high recurrence rates. Bleomycin has received widespread attention in scar treatment in recent years, but there is currently no exploration on its real-world data. PubMed, Embase, and Cochrane databases were searched, and eight retrospective studies on the use of bleomycin for treatment were included, covering a total of 562 patients with keloids and hypertrophic scars.

Integrating Ultra-High-Performance Liquid Chromatography and Orbitrap High-Resolution Mass Spectrometry, Feature-Based Molecular Networking, and Network Medicine to Unlock Harvesting Strategies for Endangered Sinocalycanthus Chinensis.

Evaluating the practical utility of endangered plant species is crucial for their conservation. Nevertheless, numerous endangered plants, including Sinocalycanthus chinensis, lack historical usage data, leading to a paucity of guidance in traditional pharmacological research. This gap impedes their development and potential utilization.

Lithium salt-derived artificial near-surface reconfiguration to stabilize high-voltage LiCoO.

Pushing the limit of the charging cut-off voltage inevitably leads to the instability of bulk and interfacial structures. Herein, one-step dual-modified LiCoO (LCO) is achieved by thermodynamic decomposition of lithiuim salts on the surface, featuring F-doped bulk and LiF & LiBO coating layers. Notably, such artificial near-surface reconfiguration effectively suppresses Co dissolution, structural deconstruction and electrolyte side reactions during repeated lithiation/delithiation processes.

Photoinduced ligand-to-metal charge transfer (LMCT) in organic synthesis: reaction modes and research advances.

In recent years, visible light-induced ligand-to-metal charge transfer (LMCT) has emerged as an attractive approach for synthesizing a range of functionalized molecules. Compared to conventional photoredox reactions, photoinduced LMCT activation does not depend on redox potential and offers diverse reaction pathways, making it particularly suitable for the activation of inert bonds and the functional modification of complex organic molecules. This review highlights the indispensable role of photoinduced LMCT in synthetic chemistry, with a focus on recent advancements in LMCT-mediated hydrogen atom transfer (HAT), C-C bond cleavage, decarboxylative transformations, and radical ligand transfer (RLT) reactions.

Nonviral targeted mRNA delivery: principles, progresses, and challenges.

Messenger RNA (mRNA) therapeutics have garnered considerable attention due to their remarkable efficacy in the treatment of various diseases. The COVID-19 mRNA vaccine and RSV mRNA vaccine have been approved on the market. Due to the inherent nuclease-instability and negative charge of mRNA, delivery systems are developed to protect the mRNA from degradation and facilitate its crossing cell membrane to express functional proteins or peptides in the cytoplasm.

Aerodigestive Sequelae and Triple Endoscopy after Congenital Tracheoesophageal Fistula Repair in Children.

Unlabelled: Children post-tracheoesophageal fistula (TEF) repair may present with chronic respiratory and gastrointestinal symptoms that can affect quality of life.

Objective: To identify factors associated with positive findings on triple endoscopy following neonatal TEF repair.

Study Design: Case series with retrospective review of patients.

Polysaccharide-based biomaterials for regenerative therapy in intervertebral disc degeneration.

Intervertebral disc (IVD) degeneration represents a significant cause of chronic back pain and disability, with a substantial impact on the quality of life. Conventional therapeutic modalities frequently address the symptoms rather than the underlying etiology, underscoring the necessity for regenerative therapies that restore disc function. Polysaccharide-based materials, such as hyaluronic acid, alginate, chitosan, and chondroitin sulfate, have emerged as promising candidates for intervertebral disc degeneration (IVDD) therapy due to their biocompatibility, biodegradability, and ability to mimic the native extracellular matrix (ECM) of the nucleus pulposus (NP).

The relationship between thigh circumference and sarcopenia in Chinese community-dwelling elderly aged ≥60 years.

Background: There has been a growing body of research demonstrating that thigh circumference is closely associated with the development of various chronic disease. However, limited evidence has been obtained regarding the relationship between thigh circumference and sarcopenia.

Objective: The aim of this study was to investigate the relationship between thigh circumference and sarcopenia, and explore the potential role of thigh circumference for sarcopenia screening among community-dwelling older adults.

Color-induced changes in : an integrative transcriptomic and metabolomic analysis of petals and non-petals.

(CM), renowned for its diverse and vibrant varieties, holds significant ornamental and medicinal value. Despite this, the core regulatory mechanisms underlying its coloration, especially in non-petal tissues (i.e.

Complete chloroplast genome data reveal the existence of the L. complex and its potential introduction pathways into China.

, native to North America, is an invasive species in many areas of the world, where it causes serious damage to natural ecosystems and economic losses. However, a dearth of genetic resources and molecular markers has hampered our understanding of its invasion history. Here, we assembled 40 complete chloroplast genomes of species, including 21 individuals, 15 individuals, and four individuals, the sizes of which ranged from 152,412 bp to 153,170 bp.

Protein Phosphatase 2A Promotes CD8 T Cell Effector Function through the Augmentation of CD28 Costimulation.

Protein phosphatase 2A (PP2A) is one of the most abundant serine/threonine phosphatases and plays critical roles in regulating cell fate and function. We previously showed that PP2A regulates the differentiation of CD4 T cells and the development of thymocytes. Nevertheless, its role in CD8 T cells remains elusive.

Peritoneal adipose stem cell-derived extracellular vesicles mediate the regulation of ovarian cancer cell proliferation and migration through EGFR-NF-κB signaling.

Peritoneal dissemination frequently develops in patients with ovarian cancer (OC) and is associated with recurrence and metastasis. However, the cellular components and mechanisms supporting OC peritoneal metastasis are poorly understood. To elucidate these, we utilized RNA sequencing to investigate the cellular composition and function.

Single-cell transcriptomic analysis of chondrocytes in cartilage and pathogenesis of osteoarthritis.

Chondrocyte is considered the only cell type in cartilage. However, the cell heterogeneity of chondrocytes in human articular cartilage is still not well defined, which hinders our understanding of the pathogenesis of osteoarthritis (OA). Here, we constructed a single-cell transcriptomic atlas of chondrocytes in healthy cartilage and identified nine chondrocyte subsets including homeostatic chondrocytes, proliferate fibrochondrocytes, and hypertrophic chondrocytes (HTC).