Endogenous cell wall degrading enzyme LytD is important for the biocontrol activity of .

Autolysins are endogenous cell wall degrading enzymes (CWDEs) in bacteria that remodel the peptidoglycan layer of its own cell wall. In the genome, at least 35 autolysin genes have been identified. However, the study of their roles in bacterial physiology has been hampered by their complexity and functional redundancy.

Genome Sequencing and Characterization of N23 as Biocontrol Agent against Plant Pathogens.

The overuse of chemical fungicides against fungal pathogens adversely affects soil and plant health, resulting in environmental problems and food safety. Therefore, biocontrol is considered as an environmentally friendly and cost-effective green technique in environmental protection and agricultural production. We obtained a bacterial strain N23 from a contaminated plate which showed significant inhibition to anthracnose.

Autotoxic Ginsenoside Stress Induces Changes in Root Exudates to Recruit the Beneficial Strain B36 as Revealed by Transcriptomic and Metabolomic Approaches.

Plants can recruit beneficial microbes to help improve their fitness under abiotic or biotic stress. Our previous studies found that could enrich beneficial sp. B36 in the rhizosphere soil under autotoxic ginsenoside stress.

Autotoxin Rg Induces Degradation of Root Cell Walls and Aggravates Root Rot by Modifying the Rhizospheric Microbiome.

Management of crop root rot disease is one of the key factors in ensuring sustainable development in agricultural production. The accumulation of autotoxins and pathogens in soil has been reported as a primary driver of root rot diseases; however, less is known about the correlation of plants, their associated pathogens and microbiome mediated by autotoxins as well as the contributions autotoxins make to the occurrence of root rot disease. Here, we integrated metabolomic, transcriptomic, and rhizosphere microbiome analyses to identify the root cell wall degradants cellobiose and d-galacturonic acid as being induced by the autotoxic ginsenoside Rg of , and we found that exogenous cellobiose and d-galacturonic acid in addition to Rg could aggravate root rot disease by modifying the rhizosphere microbiome.

Enrichment of in the Rhizosphere by Autotoxic Ginsenosides to Alleviate Negative Plant-Soil Feedback.

The accumulation of autotoxins and soilborne pathogens in soil was shown to be the primary driver of negative plant-soil feedback (NPSF). There is a concerted understanding that plants could enhance their adaptability to biotic or abiotic stress by modifying the rhizosphere microbiome. However, it is not clear whether autotoxins could enrich microbes to degrade themselves or antagonize soilborne pathogens.

Negative Plant-Soil Feedback Driven by Re-assemblage of the Rhizosphere Microbiome With the Growth of .

There is a concerted understanding of the accumulation of soil pathogens as the major driving factor of negative plant-soil feedback (NPSF). However, our knowledge of the connection between plant growth, pathogen build-up and soil microbiome assemblage is limited. In this study, significant negative feedback between the soil and sanqi () was found, which were caused by the build-up of the soil-borne pathogens , , and .