Arginase-1 promotes lens epithelial-to-mesenchymal transition in different models of anterior subcapsular cataract.

Background: Arginase-1 (ARG1) promotes collagen synthesis and cell proliferation. ARG1 is highly expressed in various tumour cells. The mechanisms of ARG1 in epithelial-to-mesenchymal transition (EMT)-associated cataracts were studied herein.

A universal SERS-label immunoassay for pathogen bacteria detection based on FeO@Au-aptamer separation and antibody-protein A orientation recognition.

Rapid, reliable and sensitive detection methods for pathogenic bacteria are strongly demanded. Herein, we proposed a magnetically assisted surface enhanced Raman scattering (SERS)-label immunoassay for the sensitive detection of bacteria by using a universal approach based on free antibody labelling and staphylococcus proteins A (PA)-SERS tags orientation recognition. The SERS biosensor consists of two functional nanomaterials: aptamer-conjugated FeO@Au magnetic nanoparticles (MNPs) as magnetic SERS platform for pathogen enrichment and PA modified-SERS tags (Au@DTNB@PA) as a universal probe for target bacteria quantitative detection.

Rapid, Quantitative, High-Sensitive Detection of O157:H7 by Gold-Shell Silica-Core Nanospheres-Based Surface-Enhanced Raman Scattering Lateral Flow Immunoassay.

O157:H7 is regarded as one of the most harmful pathogenic microorganisms related to foodborne diseases. This paper proposes a rapid-detection biosensor for the sensitive and quantitative analysis of O157:H7 in biological samples by surface-enhanced Raman scattering (SERS)-based lateral flow immunoassay (LFIA). A novel gold-shell silica-core (SiO/Au) nanosphere (NP) with monodispersity, good stability, and excellent SERS activity was utilized to prepare high-performance tags for the SERS-based LFIA system.

Sensitive and Simultaneous Detection of SARS-CoV-2-Specific IgM/IgG Using Lateral Flow Immunoassay Based on Dual-Mode Quantum Dot Nanobeads.

A rapid and accurate method for detection of virus (SARS-CoV-2)-specific antibodies is important to contain the 2019 coronavirus disease (COVID-19) outbreak, which is still urgently needed. Here, we develop a colorimetric-fluorescent dual-mode lateral flow immunoassay (LFIA) biosensor for rapid, sensitive, and simultaneous detection of SARS-CoV-2-specific IgM and IgG in human serum using spike (S) protein-conjugated SiO@Au@QD nanobeads (NBs) as labels. The assay only needs 1 μL of the serum sample, can be completed within 15 min, and is 100 times more sensitive than the colloidal gold-based LFIA.

Dual-recognition surface-enhanced Raman scattering(SERS)biosensor for pathogenic bacteria detection by using vancomycin-SERS tags and aptamer-FeO@Au.

Rapid and reliable detection of pathogenic bacteria is vital to prevent and control bacterial diseases. In this study, we present a magnetically assisted surface-enhanced Raman scattering (SERS) biosensor based on the dual-recognition of bacterial cell by aptamer and antibiotic molecules. Aptamer-FeO@Au magnetic nanoparticles (AuMNPs) were synthesized as magnetic and SERS activated substrate for specific bacteria enrichment, vancomycin-SERS tags (Au@MBA) were prepared for the sensitive quantification of pathogenic bacteria.

Rapid identification and antibiotic susceptibility test of pathogens in blood based on magnetic separation and surface-enhanced Raman scattering.

An effective surface-enhanced Raman scattering (SERS) method is presented for the rapid identification and drug sensitivity analysis of pathogens in blood. In a first step, polyethyleneimine-modified magnetic microspheres (FeO@PEI) were used to enrich bacteria from blood samples. Next, the FeO@PEI@bacteria complex was cultured on both ordinary and drug-sensitive plates.