[Silencing ERp29 promotes the invasiveness of human PCa cells in vitro: Molecular mechanisms].

Objective: To analyz the correlation of the expression of ERp29 with the clinicopathological characteristics of PCa and investigate the effect of small interfering RNA (siRNA) silencing the ERp29 gene on the biological behavior of PCa LNCaP cells.

Methods: The expression of the ERp29 gene in the BPH and PCa tissues was detected by immunohistochemistry and that of the ERp29 protein in the PCa and adjacent normal tissues of 6 PCa patients determined by Western blot. Human LNCaP cells were transfected with siRNA using LipofectamineTM 2000, and the expressions of ERp29 mRNA and protein in the LNCaP cells detected by quantitative real-time PCR (qRT-PCR) and Western blot, respectively.

[RNA interference of RelB enhances the radiosensitivity of prostate cancer cell line RM-1 in mice].

Objective: To investigate the effect of RNA interference of the RelB gene on the radiosensitivity of the mouse prostate cancer cell line RM-1 and its mechanism.

Methods: We constructed RelB siRNA-expressing lentiviral vectors targeting the RelB gene with the molecular biological technique, and determined the expressions of RelB mRNA and protein on radiation after transfection with siRelB mediated by liposome using RT-PCR and Western blot, respectively. We also detected the apoptosis of RM-1 cells by FCM assay and their radiosensitivity by clonogenic assay.

Down-regulation of EZH2 by RNA interference inhibits proliferation and invasion of ACHN cells via the Wnt/β- catenin pathway.

Although enhancer of zeste homolog 2 (EZH2) has been reported as an independent prognostic factor in renal cell carcinoma (RCC), little is known about the exact mechanism of EZH2 in promoting the genesis of RCC. However, several studies have shown that dysregulation of the Wnt/β-catenin signaling pathway plays a crucial role. Therefore, we determined whether EZH2 could affect ACHN human RCC cell proliferation and invasion via the Wnt/β-catenin pathway.

[Surgical strategy for ectopic kidneys: analysis of 35 cases].

Objective: To explore the surgical strategy for ectopic kidney and evaluate the clinical outcomes.

Methods: From January 2000 to October 2009, 35 cases of ectopic kidney were treated surgically in our hospital. Definite diagnoses were established in all the cases by ultrasound, intravenous urography (IVU), cystoscope, CT, magnetic resonance urography (MRU) and radionuclide imaging before the surgery.

[Effect of PI3K/AKT inhibitor on benign prostate hyperplasia and its mechanism: an experimental study].

Objective: To explore the effect of the phosphoinositide 3-kinase/protein kinase B (PI3K/PKB or PI3K/AKT) signaling pathway inhibitor on benign prostate hyperplasia (BPH) and its mechanism.

Methods: Forty-eight SD male adult rats aged 12 weeks were equally randomized to 4 groups: sham operation control, BPH model, 50 mg LY294002 and 100 mg LY294002. The BPH models were made by muscular injection of testosterone propionate at 10 mg/kg/d for 30 days following castration.

Clinical utility of fluorescence in situ hybridization for prediction of residual tumor after transurethral resection of bladder urothelial carcinoma.

Objectives: To evaluate the use of a multiprobe fluorescence in situ hybridization (FISH) assay for predicting the residual tumor load after transurethral resection (TUR) of bladder urothelial carcinoma (UC).

Methods: Voided urine specimens were collected from 125 consecutive patients with suspected UC who had been admitted for TUR. Of the 125 patients, 89 with UC diagnosed underwent a second procedure (repeated TUR or cystectomy) 4-6 weeks after the initial TUR and were included in the present study.

[Phloroglucinol: safe and effective for the prevention of bladder spasm after TURP].

Objective: To evaluate the efficacy of phloroglucinol in preventing bladder spasm after transurethral resection of the prostate (TURP).

Methods: Using the random sampling method, we assigned 74 cases of TURP into a treatment group (n = 39), given 80 mg phloroglucinol every day for 3 days, and a control group (n = 35), left untreated. Then we observed the frequency, duration and pain of bladder spasm within the 3 days and compared them between the two groups.

Hyperbaric oxygen therapy for recovery of erectile function after posterior urethral reconstruction.

Objective: To prospectively study the effects of hyperbaric oxygen therapy (HBOT) on the recovery of erectile function (EF) after posterior urethral reconstruction.

Methods: Between August 2006 and March 2010, a total of 24 male patients with posterior urethral reconstruction and without erectile dysfunction (ED) before urethral stricture were involved in the study. Twelve of them were assigned to HBOT group that received HBOT, and the others comprised the control group.

[Diagnosis and treatment of rare mixed prostatic carcinoma].

Objective: To discuss the diagnosis and treatment of rare mixed prostatic carcinoma.

Methods: We retrospectively analyzed the clinical data of 6 cases of mixed prostatic tumor confirmed by surgery or transrectal ultrasound guided prostate biopsy, and reviewed the related literature.

Results: Three of the patients (2 with mixed small cell carcinoma and adenocarcinoma and 1 with adenosquamous carcinoma of the prostate) underwent palliative transurethral electrovaporization of the prostate (TUVP) and endocrine therapy, but all died within 7 - 10 months.

[A clinical analysis of benign prostatic hyperplasia with chronic prostatitis].

Objective: To study the incidence of benign prostatic hyperplasia (BPH) complicated by chronic prostatitis.

Methods: We performed routine examinations and bacterial culture of the expressed prostate secretion (EPS) for 213 cases of BPH, detected mycoplasma, chlamydia and serum PSA, and compared the results of IPSS of those complicated with chronic prostatitis before and after a 4-week anti-inflammatory treatment.

Results: Of the total cases, 69 (32.

Hypothetic association between greater sympathetic activity and prostate cancer.

Among men, prostate cancer is the most common cancer diagnosed, and the second leading cause of death from cancer in the industrialized countries. In spite of substantial progress in research, diagnosis and treatment, the causes of prostate cancer remain largely unknown. In this paper, we propose the hypothesis that prostate cancer represents another entity in the constellation of disease incited by the greater sympathetic activity that develops with age.

[Urethroplasty with tunica vaginalis flap for treating hypospadias with hydrocele: a report of 9 cases].

Objective: To explore the treatment of hypospadias with hydrocele.

Methods: Nine patients with hypospadias complicated by hydrocele underwent urethroplasty with tunica vaginalis flap.

Results: Satisfactory results were achieved in 7 cases, all with a normal anatomic position of urethral meatus, perfect urination and no infection.

[Expression of Ki-67, Bcl-2,Bax and caspase-3 in benign prostatic hyperplasia combined with prostatitis and their significances].

Objective: To detect the expression of Ki-67, Bcl-2, Bax and caspase-3 in simple benign prostatic hyperplasia (BPH) and BPH combined with prostatitis,and to evaluate the effect of inflammation on the development and progression of BPH.

Methods: All specimens were obtained from patients undergoing surgical resection of the prostate. The paraffin section of the specimens was stained with hemotoxyline and eosin, and observed under light microscope to examine the inflammation hispathological changes.

[A multi-center clinical trial of Qianlieantong tablets for chronic prostatitis].

Objective: To observe the efficacy and safety of Qianlieantong Tablets in the treatment of chronic prostatitis.

Methods: A multi-center, self-controlled open clinical trial was conducted. A total of 280 subjects with chronic prostatitis were enrolled and treated by Qianlieantong Tablets, 3 times a day, 5 tablets each time.

Chronic inflammation in benign prostatic hyperplasia: implications for therapy.

Benign prostatic hyperplasia (BPH) is a common age related proliferative abnormality of the human prostate. Histological features demonstrate that the typical nodules of BPH are composed of stromal and epithelial cells that are proportionally different between nodules. BPH nodules frequently occur concurrently with chronic inflammatory infiltrates, mainly composed of chronically activated T cells and macrophages.

[Evaluation of 126 radical cystectomy with orthotopic ileal or sigmoidocolic neobladder].

Objective: To evaluate the long-term clinical effects of orthotopic ileal or sigmoidocolic neobladder.

Methods: One hundred and twenty six patients with bladder cancer who underwent radical cystectomy and orthotopic ileal or sigmoidocolic neobladder from 1989 to 2001 were followed up, the clinical data was collected and analysed. Hautmann orthotopic ileal neobladder was performed on 84 cases and orthotopic sigmoidocolic neobladder was performed on 42 cases; Lymph node clearing during surgery was performed on 62 cases, chemotherapy and radiotherapy was performed on 64 cases after surgery.

[Smad4 and TGF-beta1 expression and clinical significance in bladder transitional cell carcinoma].

Objective: To determine the expression of Smad4 and TGF-beta1 in bladder transitional cell carcinoma (BTCC), and to understand the mechanism of invasion, angiogenesis, and metastasis of BTCC.

Methods: The expressions of Smad4 and TGF-beta1 in samples of 42 human bladder carcinoma and 12 normal bladder mucosa tissues were determined with standard immunohistochemical analysis. We also analyzed the relationship among the expressions of Smad4 and TGF-beta1 and invasion, angiogenesis, and metastasis of BTCC, and the correlation between Smad4 and TGF-beta1.

[The efficacy and safety in the treatment of chronic abacterial prostatitis/chronic pelvic pain syndrome after intraprostatic injection of Chuanshentong].

Objective: To assess the clinic efficacy and safety in the treatment of chronic abacterial prostatitis/chronic pelvic pain syndrome (CABP/CPPS) after intraprostatic injection of Chuanshentong.

Methods: Five milliliter of solution blending Chuanshentong and lidocaine was transperineally injected into one lobe of prostate, once a day for 6 days, for a total of 98 cases of patients who had been diagnosed as chronic abacterial prostatitis/chronic pelvic pain syndrome. The efficacy was evaluated by the NIH Chronic Prostatitis Symptom Index (CPSI) after a 12 week follow-up.