[Normal light and fluorescence microscopy for authentication of Delphinii Brunoniani Herba of Tibet].

Dried herb of Delphinium brunonianum Royle (Ranunculaceae) has long been used under the herbal name "Xiaguobei" (Delphinii Brunoniani Herba) in traditional Tibetan medicine and prescribed for the treatment of influenza, itchy skin rash and snake bites. In order to find a useful and convenient method for the identification of microscopic features, the technique of fluorescence microscopy was applied to authenticate "Xiaguobei" of Tibet. The transverse sections of stem and leaf, as well as the powder of "Xiaguobei" were observed to seek for typical microscopic features by normal light and fluorescence microscopy.

Five new compounds from Dendrobium crystallinum.

Five new compounds, dencryol A (1), dencryol B (2), crystalltone (3), crystallinin (4), and 3-hydroxy-2-methoxy-5,6-dimethylbenzoic acid (5), together with six known compounds, dendronobilin B (6), syringic acid (7), apigenin (8), isoviolanthin (9), 6'''-glucosyl-vitexin (10), and palmarumycin JC2 (11), have been isolated from the stems of Dendrobium crystallinum, of which compounds 9-11 were isolated from the genus Dendrobium for the first time, and all the other compounds were first obtained from this plant. Their structures were established on the basis of spectroscopic analysis and literature data.

[Phenolic components from herbs of Dendrobium aphyllum].

Objective: To study the phenolic constituents of Dendrobium aphyllum.

Method: Various chromatographic techniques were used to isolate and purify the constituents, their physico-chemical properties and spectral data were employed to elucidate their structures.

Result: Nine bibenzyls and two benzylethanyl compounds were isolated and identified as: moscatilin (1), gigantol (2), batatasin (3), tristin (4), 3, 5, 4'-trihydroxylbibenzyl (5), 3, 5-dimethoxyl-4, 4'-dihydroxylbibenzyl (6), moscatin (7), 2, 4, 7-trihydroxyl-9, 10-dihydrophenanthrene (8), hircinol (9), 2-(4-hydroxyphenyl) ethyl-beta-D-glucopyranoside, salidroside (10) and p-hydroxylbenzylacetic acid (11).

[Studies on chemical constituents of Dendrobium crystallinum].

Objective: To study the chemical constituents of Dendobium crystallinum.

Method: Compounds were isolated and purified by silica gel and Sephadex LH-20 column chromatography. Their structures were identified by physicochemical properties and spectral analyses.

Two new dammarane triterpene glycosides from the rhizomes of Panax notoginseng.

Two new dammarane triterpene glycosides named notoginsenosides Rw 1 (1) and Rw 2 (2) were isolated from the rhizomes of Panax notoginseng, together with 20 known compounds including protopanaxadiol (3), protopanaxatriol (4), ginsenosides Rb1 (5), Rd (6), Re (7), Rg1 (8), Rg2 (9), 20-(S)-Rg3 (10), 20-(R)-Rg(3) (11), Rh1 (12), Rh4 (13), Rf (14), 20-O-glucopyranosyl Rf (15), notoginsenosides R1 (16), R2 (17), T5 (18), S (19), T (20), and Fa (21), and koryoginsenoside R1 (22). Based on FABMS, HRFABMS, IR, (1)H, 13C, and 2D-NMR (HSQC, HMBC, and COSY) spectral data, the structures of the new compounds were elucidated as 6-O-beta-d-xylopyranosyl-20-O-beta-d-xylopyranosyl-(1 --> 6)-beta-d-glucopyranosyldammar-24-ene-3beta,6alpha,12beta,20(S)tetraol (1) and 6-O-beta-d-xylopyranosyl-(1 --> 2)-beta-d-glucopyranosyldammar-22-ene-(trans)-3beta,6alpha,12beta,20(S), 25-pentaol (2). Compounds 3, 4, 13, and 22 were isolated from P.

[Studies on chemical constituents in stem of Dendrobium chrysotoxum].

Objective: To investigate the chemical constituents of Dendrobium chrysotoxum.

Method: The chemical constituents were isolated by various column chromatographic methods and structurally elucidated by spectral evidences.

Result: Ten compounds were obtained and identified as (+)-syringare sinol (1), 5alpha, 8alpha-epidioxy-24( R)-methycholesta-6, 22-dien-3beta-ol (2), trans-3-(4-hydroxy-3-methoxyphenyl)-acrylic acid octacosyl ester (3), defusin (4), 3, 4-dihydroxy benzoic acid (5), 3, 4-dimethoxy-benzoic acid (6), vanillic acid (7), 3, 4-dimethoxy-benzoic acid methyl ester (8), 3, 5-dibromo-2-aminobenzaldehyde (9), heptadecanoic acid 2, 3-dihydroxy-propyl ester (10).

A new phenanthrene with a spirolactone from Dendrobium chrysanthum and its anti-inflammatory activities.

Investigation of phenolic patterns from the stems of Dendrobium chrysanthum by HPLC-PDA-MS has led to the isolation of a new phenanthrene derivative with a spirolactone ring, dendrochrysanene (1), that proved to suppress the mRNA level of TNF-alpha, IL8, IL10, and iNOS in murine peritoneal macrophages. The structure of 1 was characterized on the basis of various NMR (1H, 13C, 1H-1H COSY, HMQC, and HMBC), mass spectrometry, and X-ray crystal diffraction data.

[Molecular identification of medicinal plants: Dendrobium chrysanthum, Dendrobium fimbriatum and their morphologically allied species by PCR-RFLP analyses].

Aim: To establish a simple method for molecular identification of original plants of D. chrysanthum and D. fimbriatum using molecular marker rDNA ITS region.

[Location and relative quantity of coumarins in the stem of Dendrobium thyrsiflorum].

Aim: To determine the location and relative quantity of coumarins in the stem of Dendrobium thyrsiflorum Rchb. f. , and to provide a scientific basis for evaluating and utilizing the famous medicinal plant.

Bi-bicyclic and bi-tricyclic compounds from Dendrobium thyrsiflorum.

One bi-bicyclic and two bi-tricyclic derivatives of coumarin-benzofuran, phenanthrene-phenanthrene and phenanthrene-phenanthraquinone, along with seven known compounds, were isolated from stems of Dendrobium thyrsiflorum Rchb.f. (Orchidaceae).

[Analysis on the geologic background and physicochemical properties of soil for the cultivation of Panax notoginseng in Yunnan province].

Objective: To study the relationship between the geo-authentic and geologic background system (GBS) and physicochemical properties of soil for the cultivation of Panax notoginseng.

Method: The physicochemical properties of soil were analyzed, the geologic background system between producing areas of Yunnan and Guanxi of P. notogiseng was compared.

[Studies on chemical constituents of Pholidota yunnanensis].

Objective: To investigate the chemical constituents from Pholidota yunnanensis.

Method: Various chromatographic techniques were employed for isolation and purification of the constituents. The structures were elucidated by chemical and spectral analyses.

[Determination of linderane in root tuber of Lindera aggregata by HPLC].

Objective: To provide scientific basis for quality control of Lindera aggregata.

Method: HPLC analytical method was established using a Lichrospher C18 column and acetonitrile-water (56:44) as the mobile phase, detected at 235 nm.

Result: The linear range of linderane is between 0.

Two new fluorenones from Dendrobium chrysotoxum.

Two new fluorenones have been isolated from the orchid Dendrobium chrysotoxum Lindl. and their structures established as 2,4,7-trihydroxy-5-methoxy-9-fluorenone and 2,4,7-trihydroxy-1,5-dimethoxy-9-fluorenone.

[Studies on the chemical constituents of Dendrobium fimbriatum].

Aim: To investigate the chemical constituents of Dendrobium fimbriatum Hook.

Methods: Various chromatographic techniques were employed for isolation and purification of the constituents. The structures were elucidated by IR, MS, 1HNMR, 13CNMR and 2D-NMR.

[Analysis of 1H-NMR fingerprint in stem of Dendrobium loddigesii].

Objective: To analyse the 1H-NMR finger-print of the stem of Dendrobium loddigesii.

Method: Silica gel column chromatography was used to separate the chemical constituents of SCE A of the stem of D. loddigesii.

[Studies on chemical constituents of Alpinia jianganfeng].

Objective: To study the chemical constituents of Alpinia jianganfeng (Zingiberaceae) distributed in Sichuan province.

Method: Chromatography and spectral analyses were used to isolate the constituents and elucidate their structure.

Result: Four compounds were isolated from the rhizome of A.

[Studies on chemical constituents of two plants from Costus].

Objective: To study the chemical constituents of Costus speciosus and C. tonkinensis (Zingiberaceae) distributed in Yunnan province.

Method: Chromatography and spectral analyses were used to isolate the constituents and elucidate their structure.

[Pharmacognostical identification of "guijiu ([symbol: see text])" in Shosoin of Japan].

Objective: To identify the botanical origin of the Guijiu in Shosoin of Japan from Tang Dynasty, and trace its medicinal history.

Method: Anatomical characteristics of the underground parts of Guijiu in Shosoin were compared with those of Hosta plantaginea and H. ventricosa, and research on the medicinal history of Guijiu was made based on its original identification results and describes in herbals.

[Study on sequence difference and SNP pheomenon of rDNA ITS region in F type and H type population of Dendrobium officinale].

Objective: To study rDNA ITS sequence differences between F type and that of H type of Dendrobium officinale in main habitat of China.

Method: The population differences of the rDNA ITS region (including ITS1, ITS2, 5.8S) sequences of D.

beta-Carboline alkaloids from hypodematium squamuloso-pilosum.

A new beta-carboline alkaloid, 1-acetyl-8-hydroxy-beta-carboline, was isolated from the fern, Hypodematium squamuloso-pilosum, along with a known compound, 1-acetyl-beta-carboline. Their structures were elucidated from spectral evidence. This is the first report of this type of alkaloid being found in ferns.