Exploring the molecular mechanism of estrogen therapy effectiveness after TCRA in IUA patients at single-cell level.

Background: Intrauterine adhesion (IUA) is a common cause of clinically refractory infertility, and there exists significant heterogeneity in the treatment outcomes among IUA patients with the similar severity after transcervical resection of adhesion(TCRA). The underlying mechanism of different treatment outcomes occur remains elusive, and the precise contribution of various cell subtypes in this process remains uncertain.

Results: Here, we performed single-cell transcriptome sequencing on 10 human endometrial samples to establish a single-cell atlas differences between patients who responded to estrogen therapy and those who did not.

A study on the knowledge, attitude, and practice of research integrity among medical professionals in Ningxia, China.

Background: This study utilizes a Knowledge, Attitude, and Practice (KAP) questionnaire to investigate the awareness of medical professionals in the Ningxia region regarding research integrity and their attitudes towards academic misconduct. A comprehensive analysis of the factors contributing to academic misconduct is performed, with the aim of providing insights for the formulation of policies to enhance research integrity.

Methods: This study is a cross-sectional study.

Protective effects of puerarin on metabolic diseases: Emphasis on the therapeutical effects and the underlying molecular mechanisms.

Metabolic diseases (MetD) such as diabetes mellitus, obesity, and hyperlipidemia have become global health challenges. As a naturally occurring plant component, puerarin has been verified to possess a wide range of pharmacological effects including lowering blood glucose, improving insulin resistance, and regulating lipid metabolism, which has attracted extensive attention in recent years, and its potential in the treatment of MetD has been highly acclaimed. In addition, puerarin has exhibited antioxidant, anti-inflammatory, and cardiovascular protective effects, which are of great significance in the prevention and treatment of MetD.

Interleukin-33 promotes intrauterine adhesion formation in mice through the mitogen-activated protein kinase signaling pathway.

IL-33 belongs to the inflammatory factor family and is closely associated with the inflammatory response. However, its role in the development of intrauterine adhesions (IUAs) remains unclear. In this study, the role of IL-33 in the formation of IUAs after endometrial injury was identified via RNA sequencing after mouse endometrial organoids were transplanted into an IUA mouse model.

The beneficial health effects of puerarin in the treatment of cardiovascular diseases: from mechanisms to therapeutics.

Cardiovascular diseases (CVDs) are the leading causes of death globally that seriously threaten human health. Although novel western medicines have continued to be discovered over the past few decades to inhibit the progression of CVDs, new drug research and development for treating CVDs with less side effects and adverse reactions are continuously being desired. Puerarin is a natural product found in a variety of medicinal plants belonging to the flavonoid family with potent biological and pharmacological activities.

Laboratory and Field Performance Evaluation of High-Workability Ultra-Thin Asphalt Overlays.

The defects of poor workability and inadequate pavement performance of the ultra-thin asphalt overlay limited its application in the preventive maintenance of pavements. In this study, a high-workability ultra-thin (HWU) asphalt overlay scheme was proposed. A high-strength-modified asphalt binder and an optimized HWU-10 gradation were used to prepare the HWU asphalt mixture and explore its laboratory performance.

MiR-126-5p Promotes Tumor Cell Proliferation, Metastasis and Invasion by Targeting TDO2 in Hepatocellular Carcinoma.

TDO2 is a key enzyme in the kynurenine metabolic pathway, which is the most important pathway of tryptophan metabolism. It has been shown that miRNAs are involved in cell metastasis through interaction with target mRNAs. In this study, we found 645 miRNAs that could be immunoprecipitated with TDO2 through the RNA-immunoprecipitation experiment.

Changes in the hepatic differentiation potential of human mesenchymal stem cells aged .

Background: Due to their multipotency and ability for self-renewal, human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) hold great promise for generating hepatocytes. Previous research has successfully generated hepatocytes from early-passage [i.e.

Tryptophan Side-Chain Oxidase Enzyme Suppresses Hepatocellular Carcinoma Growth through Degradation of Tryptophan.

Tryptophan metabolism plays a role in the occurrence and development of hepatocellular carcinoma cells. By degrading certain amino acids, tumor growth can be limited while maintaining the body's normal nutritional requirements. Tryptophan side-chain oxidase (TSO) enzyme can degrade tryptophan, and its inhibitory effect on hepatocellular carcinoma cells is worthy of further study.

Functional hit 1 (FH1)-based rapid and efficient generation of functional hepatocytes from human mesenchymal stem cells: a novel strategy for hepatic differentiation.

Background: Because the liver is central to the physiology of the body, primary hepatocytes are widely used in liver pathology and physiological research, such as liver drug screening, bioartificial liver support system, and cell therapy for liver diseases. However, the source of primary hepatocytes is limited. We describe a novel non-transgenic protocol that facilitates the rapid generation of hepatocyte-like cells from human umbilical cord-derived mesenchymal stem cells (hUC-MSCs), providing a new source of functional hepatocytes.

Safety assessment of subtilisin QK in rats.

Background: Subtilisin QK is a serine protease in the subtilisin family, and is fermented by Bacillus subtilis QK02. The fibrinolytic activity of subtilisin QK was measured by detecting low molecular weight degradation products using a spectrophotometric method developed by Japan Bio Science Laboratory Co., Ltd.

Determination of Construction Parameters of Porous Ultra-Thin Overlays Based on Laboratory Compaction Studies.

To address the severe distresses of asphalt pavement, a new type of pavement maintenance treatment, porous ultra-thin overlay (PUTO) with small particle size was proposed. The PUTO has a thickness of 1.5-2.

Microstructural Characteristics of Interfacial Zone in Asphalt Mixture Considering the Influence of Aggregates Properties.

The interfacial zone between aggregate particles and asphalt mortar presents a significant effect on the strength of an asphalt mixture. In this paper, basalt, limestone, and diabase were selected, and the influence of these aggregates on the mechanical characteristics and microstructures of the interfacial zone was investigated. Nanoindentation was employed to measure the change law of mechanical behavior in the region of the interfacial zone, and corresponding viscoelastic parameters were deduced; microstructural morphology was observed by scanning electron microscopy, and the effect of the mineralogical components on the interfacial zone was analyzed as well.

Emission analysis of recycled tire rubber modified asphalt in hot and warm mix conditions.

The hazardous emissions of crumb rubber (CR) modified asphalt during construction has been a concern for a long period. This study aims to identify the emission components in the CR modified asphalt in traditional hot mix asphalt (HMA) and with recently developed warm mix asphalt (WMA). The dynamic headspace gas chromatography-mass spectrometry (GCMS) was employed for identifying the emission of asphalt binders at 120°C, 140°C and 160°C.

Reproductive and locomotory capacities of Caenorhabditis elegans were not affected by simulated variable gravities and spaceflight during the Shenzhou-8 mission.

Reproduction and locomotion are essential features of animals that help to facilitate their interaction with the surrounding environment. Previous studies have produced inconsistent results on behavioral response to spaceflight by the model animal Caenorhabditis elegans (C. elegans) in liquid culture.

Prevalence and risk factors for eye diseases, blindness, and low vision in Lhasa, Tibet.

Aim: To determine the prevalence and risk factors for eye diseases, blindness, and low vision in Tibet, and to assist the development of eye disease prevention and treatment schemes.

Methods: We carried out a survey of eye diseases among a population living at high altitude. A total of 1 115 Tibetan permanent residents aged 40 years or older from the towns and villages of Qushui County, Lhasa Prefecture, Tibet Autonomous Region, participated in this study.

High altitude adaptation of the schizothoracine fishes (Cyprinidae) revealed by the mitochondrial genome analyses.

The schizothoracine fishes, also known as "mountain carps" are widely distributed in the Qinghai-Tibetan Plateau and its peripheral regions. Although they provide a prime example of high altitude adaptation, the phylogenetic relationships and the divergence times among these carp lineages are still controversial. Moreover, the genetic basis for high altitude adaptation is also poorly understood.

[An epidemiological analysis on malaria in Linzhi district of Tibet in 1986-2004].

Objective: To investigate the epidemiological characters of malaria in Linzhi district of Tibet.

Methods: A retrospective analysis on the epidemiology of malaria was carried out using the data on malaria situation in Linzhi district of Tibet in 1986-2004, referring to the distribution of season, population and region.

Results: The accumulative number of malaria cases in the period of 1986-2004 was 2459.