Mechanisms of Staphylococcus aureus Antibiotics Resistance Revealed by Adaptive Laboratory Evolution.

Infection caused by drug-resistant Staphylococcus aureus is a serious public health and veterinary concern. Lack of a comprehensive understanding of the mechanisms underlying the emergence of drug-resistant strains, it makes S. aureus one of the most intractable pathogenic bacteria.

Genetic Causal Association Between Skin Microbiota and Biological Aging: Evidence From a Mendelian Randomization Analysis.

Background: The skin microbiota, a complex community of microorganisms residing on the skin, plays a crucial role in maintaining skin health and overall homeostasis. Recent research has suggested that alterations in the composition and function of the skin microbiota may influence the aging process. However, the causal relationships between specific skin microbiota and biological aging remain unclear.

Impact of white matter hyperintensity location on outcome in acute ischemic stroke patients: a lesion-symptom mapping study.

Background: Studies on the impact of white matter hyperintensity (WMH) on function outcome have primarily concentrated on WMH volume, overlooking the potential significance of WMH location. This study aimed to investigate the relationship between WMH location and outcome in patients with their first-ever acute ischemic stroke (AIS).

Methods: Patients who underwent their first AIS between September 2021 and September 2022 were recruited.

Network analysis of intergenerational transmission of psychopathological symptom among adolescents: A cross-sectional study.

The intergenerational transmission of psychopathology has been well documented, but limited studies have examined the link at the symptomatic level accounting for these associations. This study aimed to identify the central symptoms that bridge adolescents and parental psychopathological symptoms and the specific symptom pathways by using a novel network approach. From September to October 2021, a cross-sectional study was conducted in Wuhan, China.

Multifunctional DNA-Metal Nanohybrids Derived From DNA-MgPPi Microhybrids by Rolling Circle Amplification.

Rolling circle amplification (RCA)-derived ultra-long DNA is highly attractive and versatile because of its diverse functionalities conferred by repeated DNA nanostructures. However, magnesium pyrophosphate (MgPPi) crystals, as byproducts of RCA, electrostatically interact with the DNA to form DNA microhybrids and hamper its broad bioapplications, as its large size is unfavorable for cellular uptake and decreases the density of functional DNA nanostructures. In this study, finely tuned synthesis strategies are developed to condense the microhybrids and replace non-functional MgPPi crystals with various functional metal nanostructures by reducing metal ions.

Elevated CO and goethite inhibited anaerobic oxidation of methane in paddy soils.

Microbially mediated anaerobic oxidation of methane (AOM) regulates methane (CH) fluxes. Increases in the global atmospheric carbon dioxide (CO) concentration and iron oxide rich in paddy soils influence AOM. However, the response and mechanisms between these two processes and AOM remain unclear.

Repair effect analysis of mesenchymal stem cell conditioned media from multiple sources on HUVECs damaged by high glucose.

Background: The therapeutic potential of mesenchymal stem cells (MSCs) may be partly attributed to their secretion growth factors, cytokines and chemokines. In various preclinical studies, the use of MSC-conditioned media (CM) has demonstrated promising potential for promoting vascular repair.

Methods: To gain a comprehensive understanding of the variations in conditioned media derived from different sources of mesenchymal stem cells (MSCs) including umbilical cord, adipose and bone marrow, we investigated their reparative effects on human umbilical vein endothelial cells (HUVECs) subjected to damage induced by high glucose.

Gut Microbiota: An Important Participant in Childhood Obesity.

Increasing prevalence of childhood obesity has emerged as a critical global public health concern. Recent studies have challenged the previous belief that obesity was solely a result of excessive caloric intake. Alterations in early-life gut microbiota can contribute to childhood obesity through their influence on nutrient absorption and metabolism, initiation of inflammatory responses, and regulation of gut-brain communication.

HIV self-management and associated factors among people living with HIV in Hunan, China: a nine-year longitudinal study.

Self-management refers to the behaviors and strategies individuals employ to manage their health conditions. For people living with HIV (PLWH), it encompasses medication adherence, health monitoring, symptom management, lifestyle maintenance and seeking emotional support. While previous studies on the factors influencing self-management in PLWH are predominantly cross-sectional, longitudinal studies are scarce.

Correction: Prevalence of lymphopenia in the American population: Insights from demographic, BMI, and lifestyle factors.

[This corrects the article DOI: 10.1371/journal.pone.

Improving Adherence of Young Male Patients with HBV Infection to the Regular Follow-Up via Mobile Healthcare Platform Might Be Cost-Effective to Decrease the Morbidity of Advanced Liver Cancer.

Background: Young adults contribute substantially to the social economy. However, the number of young adults with liver cancer has increased recently. In addition, the mortality rate of these patients is high.

miRNAs modulate altered expression of cytochrome P450s and nicotinic acetylcholine receptor subunits conferring both metabolic and target resistance to sulfoxaflor in Nilaparvata lugens (Stål).

Understanding the insecticide resistance mechanisms and their underlying regulatory pathways is essential for pest management. Previous findings indicated that the overexpression of P450 gene, CYP6ER1, was a key mechanism for sulfoxaflor metabolic resistance in Nilaparvata lugens. However, it remains unclear whether quantitative changes in the target nicotinic acetylcholine receptors (nAChRs) contribute to sulfoxaflor resistance and the underlying regulatory mechanisms involved.

Predicting nursing workload in digestive wards based on machine learning: A prospective study.

Background: The process of assessing and allocating nursing staff, as well as evaluating performance, relies heavily on nursing workload, which is strongly associated with patient safety outcomes. Nevertheless, most previous studies have utilized cross-sectional data collection methods, which limit the precision of workload prediction. Static workload models do not incorporate longitudinal changes in influential factors, potentially resulting in delayed or erroneous nursing management decisions and ultimately causing imbalances in nurses' workload.

Effects of childhood trauma on sustained attention in major depressive disorder: the mediating role of brain activity and functional connectivity.

Background: Sustained attention deficits were reported more significant in patients with major depressive disorder (MDD) than in healthy controls (HCs), and are pivotal in both the development and aggravation of depression. Childhood trauma is also common in MDD and the exposure to childhood trauma may impede sustained attention and increase the treatment resistance in MDD. However, the underlying neuro-mechanisms link the childhood trauma to sustained attention deficits in MDD remain unclear.

[Primary uterine hepatoid adenocarcinoma: Clinicopathological analysis of 2 cases and literature review].

Primary hepatoid adenocarcinoma (HAC) of the uterus is a particular tumour that bears high similarity to hepatocellular carcinoma histologically, and may easily be misdiagnosed because it is rare if you don' t remember it. In this report, we describe two cases of alpha-fetoprotein (AFP)-producing HAC of the uterus. Case 1 was a 69-year-old postmenopausal woman who was presented to the hospital for a medical examination.

Microenvironment-responsive injectable hydrogel for neuro-vascularized bone regeneration.

Bone is a richly innervated and vascularized tissue, whereas nerve-vascular network reconstruction was often ignored in biomaterial design, resulting in delayed or incomplete bone healing. Inspired by the bone injury microenvironments, here we report a controllable drug delivery strategy using a pH and reactive oxygen species (ROS) dual-response injectable hydrogel. Based on the dynamic borate ester bond covalent crosslinking, nano-hydroxyapatite (HA) and curculigoside (CCG) are integrated into PVA/TSPBA (PT) to construct a responsive injectable hydrogel (PTHC), which scavenges excessive ROS from the injury microenvironment and responsively releases HA and CCG, providing favorable homeostasis and sustained release drug delivery system for bone repair.

Bioengineered Extracellular Vesicle Hydrogel Modulating Inflammatory Microenvironment for Wound Management.

Chronic wounds, frequently arising from conditions like diabetes, trauma, or chronic inflammation, represent a significant medical challenge due to persistent inflammation, heightened infection risk, and limited treatment solutions. This study presents a novel bioengineered approach to promote tissue repair and improve the healing environment. We developed a bioactive hydrogel patch, encapsulated zeolitic imidazolate framework-8 (ZIF-8) into extracellular vesicles (EVs) derived from anti-inflammatory M2 macrophages, and synthesized ZIF@EV, then embedded it in the sodium alginate matrix.

Run-Mu-Ling Granules Mitigate Ocular Surface Inflammatory Injury Associated with Dry Eye by Suppressing the NLRP3/GSDMD-Mediated Pyroptosis Pathway.

Purpose: Run-Mu-Ling granules (RMLG), a traditional Chinese medicinal formula, are used to treat dry eye (DE); however, the underlying mechanism remains poorly understood. This study aimed to elucidate the potential molecular mechanisms by which RMLG alleviates ocular surface inflammation in DE.

Methods: We established an in-vivo DE rat model and in-vitro human corneal epithelial cell line (HCEC) injury models.

Analysis of differentially methylated sites and regions associated with intrauterine transmission of hepatitis B virus in infants.

Background: The goal is to identify methylation sites linked to transmission and their impact on host gene expression and HBV spread, aiming to uncover new molecular targets for preventing and treating intrauterine HBV infection.

Methods: This study recruited 1205 infants born to HBsAg-positive mothers in Liuzhou City, China, between July 2023 and January 2024. Infants were followed up at 7-12 months of age and classified as HBsAg-positive (case, n = 5) or HBsAg-negative (control, n = 14) based on serological testing.

UGCG promotes chemoresistance and breast cancer progression via NF-κB and Wnt/β-catenin pathway activation.

Background: Taxane-based chemotherapy is the primary treatment for triple-negative breast cancer (TNBC), yet clinical outcomes remain unsatisfactory due to the persistence of chemoresistance. Identifying key factors that contribute to chemoresistance and understanding the associated molecular mechanisms is therefore essential.

Method: The GEO databases were utilized to pinpoint factors related to chemoresistance, which were subsequently validated using clinical tissue samples.

A longitudinal study of resilience and social function in patients with colorectal cancer and stomas.

Objective: To explore the dynamic changes, influencing factors, and relationships between resilience and social function in patients with colorectal cancer (CRC) and stomas at different postoperative stages, and to inform precise psychosocial rehabilitation interventions.

Methods: A longitudinal study was conducted at a tertiary hospital in eastern China from January 2021 to June 2023. Patients completed a self-designed socio-demographic questionnaire one month post-surgery, and the Connor and Davidson Resilience Scale (CD-RISC) and Social Dysfunction Screening Scale (SDSS) at 1, 3, and 5 months post-surgery.