Functional identification of mango MiEXPLA1a2 and MiEXPA4e1 genes in transgenic Arabidopsis and tomato.

Expansin (EXP) is an intrinsic regulator of plant cell expansion, and have been shown to play a role in each stages of plant growth and development. But has not yet been fully studied in mango. In this experiment, two pairs of homologous genes MiEXPA1s and MiEXPA4s were firstly excavated from mango genome.

Post-operative Fluid Restriction to Prevent Delayed Hyponatremia after Endoscopic Transsphenoidal Surgery.

Background: Readmission following endoscopic endonasal transsphenoidal surgery (EETS) for pituitary neuroendocrine tumor (PitNET) and other sellar pathology is most commonly due to delayed hyponatremia. Studies suggest post-operative fluid restriction (FR) reduces delayed hyponatremia. We present a prospective randomized controlled study evaluating post-EETS FR.

Clinical application of repetitive transcranial magnetic stimulation in the treatment of chronic pelvic pain syndrome: a scoping review.

Introduction: Chronic pelvic pain syndrome is a common condition characterized by persistent symptoms that are difficult to treat. Repetitive transcranial magnetic stimulation (rTMS) is considered a safe treatment option for alleviating chronic pelvic pain, but different stimulation protocols can affect pain relief outcomes. Establishing an optimal stimulation protocol can enhance the uniformity and consistency of rTMS to provide a potentially effective therapeutic intervention.

Validation of machine learning application for the identification of lipid metabolism-associated diagnostic model in ischemic stroke.

Introduction: Ischemic Stroke (IS) is characterized by complex molecular alterations involving disruptions in lipid metabolism and immune interactions. However, the roles of lipid metabolism-associated genes in the pathogenesis of IS through immune regulation interaction are rarely explored. In this study, we aimed to explore the intricate correlation between lipid metabolism-associated immune changes and IS through a machine-learning algorithm.

Differences in plasma metabolome between non-Hispanic White and non-Hispanic Black women.

Background: To understand potential racial differences in disease susceptibility and develop targeted prevention strategies, it is essential to establish biological differences between racial groups in healthy individuals. However, knowledge about how race impacts metabolites is limited. We therefore performed a cross-sectional study using comprehensive metabolomics analysis to investigate racial differences in metabolites among 506 non-Hispanic White (NHW) women and 163 non-Hispanic Black (NHB) women.

Epiphytic bacterial consortia drive growth regulation in potato under methyl jasmonate elicitation: A leaf surface multi-omics perspective.

Methyl jasmonate (MeJA), a lipid-derived signaling molecule widely reported as a plant growth regulator, was revealed in this study to coordinate oxidative stress adaptation and delay senescence in potato through metabolite-microbe interactions, ultimately improving yield. MeJA triggered leaf oxidative stress while integrating rapid enzymatic scavenging, sustained osmoprotectant accumulation, and membrane stabilization, effectively delaying senescence initiation. Metabolic reprogramming under MeJA suppressed endogenous jasmonic acid synthesis while promoting saturated fatty acid biosynthesis, altering leaf surface lipid composition.

The Basilar Artery Collateral Simplified Score: a Novel Collateral Circulation Score Associated With Outcomes in Patients With Basilar Artery Occlusion: Results From the ATTENTION Study.

Background: The aim of the current study was to develop a simplified collateral score for basilar artery occlusion and assess its impact on the relationship between procedure time and outcomes.

Methods And Results: The derivation cohort (n=221) from the ATTENTION (Endovascular Treatment for Acute Basilar Artery Occlusion) study was used. Variables identified by logistic regression were used to develop the Basilar Artery Collateral Simplified Score (BACSS).

Molecular epidemiological study on tick-borne pathogens in Qinghai Province, Northwestern China.

Recently, there has been a continuous stream of reports on emerging tick-borne pathogens affecting humans. Qinghai Province, located in the northweastern region, is one of China's major pastoral areas, providing a suitable environment for ticks' survival and transmitting tick-borne pathogens. Here, we collected 560 free-living and parasitic ticks from 11 locations in Qinghai Province using the flag-drag method or tweezers, identifying them as belonging to 4 species of ticks.

Investigating visual perception abilities in flight cadets: the crucial role of the lingual gyrus and precuneus.

Introduction: In aviation, exceptional visual perception is crucial for pilots to monitor flight instruments and respond swiftly to deviations, as well as make rapid judgments regarding environmental changes, ensuring aviation safety. However, existing research on pilots' visual perception has predominantly focused on behavioral observations, with limited exploration of the neurophysiological mechanisms involved.

Methods: This study aimed to investigate the brain activity associated with the visual perception capabilities of flight cadets.

Functional identification of mango MiGID1A and MiGID1B genes confers early flowering and stress tolerance.

The GIBBERELLIN INSENSITIVE DWARF1 (GID1) gene encodes a receptor integral to Gibberellic acid (GA) signaling, which is pivotal for plant growth, development, and stress responses. Until now, GID1 genes have not been documented in mango. In this research, the mango (Mangifera indica) genome yielded four GID1 homologous genes, and this study focuses on the research of MiGID1A and MiGID1B genes.

Analysis of the therapeutic efficacy of OLIF combined with posterior percutaneous pedicle screw fixation in the treatment of patients with primary lumbar spondylodiscitis.

Introduction: Primary lumbar spondylodiscitis is a serious condition with an increasing incidence rate.

Methods: From January 2021 to June 2023, we adopted a single-stage oblique lateral approach for the debridement of lumbar infection foci, intervertebral bone grafting fusion, tube irrigation drainage, combined with posterior percutaneous pedicle screw fixation for the treatment of primary lumbar spondylodiscitis.

Results: We found that this surgical technique significantly improved the patients' lower back pain symptoms.

Identification of active ingredients from Lamiophlomis rotata that increase wound repair in mouse skin.

Background And Purpose: We previously showed that the iridoid glycoside extract of Lamiophlomis rotata (IGLR) induces M2 macrophage polarisation to accelerate wound healing. The potentially active compounds (quality markers) in IGLR that accelerate wound healing have not been elucidated; we here identified quality markers that accelerate wound healing.

Experimental Approach: After IGLR gavage of dorsal excisional wound mice and normal mice for 7 days, the tissues were analysed using the matrix-assisted laser desorption/ionisation (MALDI) mass spectrometry imaging (MSI) method and were further validated by liquid chromatography/mass spectrometry (LC/MS) coupled with multivariate analyses to identify quality markers.

NEK2 inhibition reverses vascular remodeling in pulmonary arterial hypertension associated with congenital heart disease.

Objective: Pulmonary arterial hypertension (PAH) is a serious consequence of congenital heart disease (CHD). PAH is characterized by a cancer-like pro-proliferative and anti-apoptotic phenotype of pulmonary artery smooth muscle cells (PASMCs). Never in mitosis a-related kinase 2 (NEK2) has recently been identified as a key factor in tumor cell proliferation and migration whlie the functional importance of NEK2 in PAH associated with CHD (CHD-PAH) has not been elucidated yet.

Neuroprotective effects of hirudin against cerebral ischemia-reperfusion injury via inhibition of CCL2-mediated ferroptosis and inflammatory pathways.

Cerebral ischemia-reperfusion injury (CIRI) is a leading cause of neurological impairment in stroke, primarily correlated to oxidative stress, inflammation, and ferroptosis. This study investigates the neuroprotective effects of hirudin on CIRI, focusing on its role in modulating neuronal survival, oxidative stress, and ferroptosis markers through inhibition of CCL2. A middle cerebral artery occlusion (MCAO) model in mice and an oxygen-glucose deprivation/reoxygenation (OGD/R) model in HT22 cells were used to simulate ischemic conditions.

A Novel Prognostic Risk-Scoring Model Based on RAS Gene-Associated Cluster in Pediatric Acute Myeloid Leukemia.

Background: With the rapid development of diagnostic techniques and treatment strategies, there are notable improvements in pediatric acute myeloid leukemia (AML) prognosis. Nevertheless, the pathogenesis of AML remains largely unknown. This study aims to investigate the RAS pathway-associated genes based on bioinformatics analysis, and investigate their underlying mechanisms in the initiation and progression of AML.

Beyond the tumor region: Peritumoral radiomics enhances prognostic accuracy in locally advanced rectal cancer.

Background: The peritumoral region possesses attributes that promote cancer growth and progression. However, the potential prognostic biomarkers in this region remain relatively underexplored in radiomics.

Aim: To investigate the prognostic value and importance of peritumoral radiomics in locally advanced rectal cancer (LARC).

Scaling up spatial transcriptomics for large-sized tissues: uncovering cellular-level tissue architecture beyond conventional platforms with iSCALE.

Recent advances in spatial transcriptomics (ST) technologies have transformed our ability to profile gene expression while retaining the crucial spatial context within tissues. However, existing ST platforms suffer from high costs, long turnaround times, low resolution, limited gene coverage, and small tissue capture areas, which hinder their broad applications. Here we present iSCALE, a method that predicts super-resolution gene expression and automatically annotates cellular-level tissue architecture for large-sized tissues that exceed the capture areas of standard ST platforms.

Epstein-Barr virus hijacks histone demethylase machinery to drive epithelial malignancy progression through KDM5B upregulation.

Epstein-Barr virus (EBV) is a significant epigenetic driver in the development of epithelial-origin nasopharyngeal carcinoma (NPC) and gastric cancer (GC), which together represent 80% of EBV-associated malignancies. Despite its known association, the specific mechanisms, particularly those involving EBV-induced histone modifications, remain poorly understood. Through integrative analyses of single-cell and bulk transcriptome data from epithelial tumor tissues and EBV-infected cells, we identified KDM5B as a critical histone-modifying factor consistently upregulated following EBV infection.

Ultrahigh antipsychotics selective accumulation and efficient photocatalytic degradation using a novel 2D BiOIO-based molecularly imprinted photocatalyst.

Adsorption-assisted photocatalytic degradation of pollutants is an effective method to improve degradation efficiency. However, most adsorptive photocatalysts are not selective and cannot efficiently remove low-concentration targets in complex systems, which limit their practical application. Therefore, a novel molecularly imprinted photocatalyst (MI-BiOIO) with high selectivity and adsorption capacity was prepared using two-dimensional BiOIO nanosheet as the matrix.

Ketogenic diets and β-hydroxybutyrate in the prevention and treatment of diabetic kidney disease: current progress and future perspectives.

Diabetic kidney disease (DKD) is the main cause of end-stage renal disease. Ketogenic diets (KD) is a high-fat, low-carbohydrate diet. KD produces ketone bodies to supplement energy in the case of insufficient glucose in the body.

An Intervertebral Disc (IVD) Regeneration Model Using Human Nucleus Pulposus Cells (iHNPCs) and Annulus Fibrosus Cells (iHAFCs).

Intervertebral disc (IVD) degeneration (IVDD), primarily caused by nucleus pulposus (NP) dehydration, leads to low back pain. While current treatments focus on symptom management or surgical intervention, tissue engineering using IVD-derived cells, biofactors, and scaffolds offers a promising regenerative approach. Here, human NP cells (NPCs) and annulus fibrosus cells (AFCs) are immortalized with human telomerase reverse transcriptase (hTERT), generating immortalized NPCs (iHNPCs) and AFCs (iHAFCs).

FN1 Immunoregulation in Glioblastoma: Insights From Neutrophil-Centric Studies.

Neutrophils, as key components of the tumor microenvironment, play a crucial role in cancer progression and prognosis. This study aimed to identify a neutrophil-related gene signature to improve prognostic predictions and explore potential immunotherapy targets for glioblastoma multiforme (GBM) patients. Through co-expression analysis, 60 neutrophil-associated genes were identified, showing significant enrichment in 159 Gene Ontology terms and eight KEGG pathways.

Application of Mini-CEX Evaluation Combined With Segmented Teaching Approach in the Training of Surgical Clinical Practice Skills for Resident Physicians.

Objective: To evaluate the impact of integrating Mini-CEX assessment with Segmented teaching on the improvement of surgical clinical practice skills among first-year resident physicians, and to compare its effectiveness with traditional teaching methods.

Methods: A total of 42 first-year resident physicians undergoing standardized training at the First Affiliated Hospital of Hengyang Medical School, University of South China from April to June 2024, who were participating in surgical clinical practice skills training for excision of superficial tumors, were selected as subjects. The participants were randomly divided into 2 groups: an experimental group and a control group, with 21 participants in each.

Ferroelectric Tunable Nonvolatile Polarization Detection Based on 2H α-InSe.

The development of polarization photodetectors utilizing two-dimensional materials holds significant promise due to their distinctive properties and potential for high integration. However, a major limitation of most current polarization photodetectors is their lack of polarization tuning capability, which restricts the versatility of individual devices. In this study, a nonvolatile ferroelectric tunable polarization photodetector is explored based on 2H α-InSe.

Development of a PANoptosis-related LncRNAs for prognosis predicting and immune infiltration characterization of gastric Cancer.

PANoptosis is a newly discovered form of programmed cell death (PCD), involving the interaction of cellular pyroptosis, apoptosis, and necroptosis. Although PANoptosis plays a significant role in carcinogenesis process, the impact of PANoptosis-related lncRNAs (PANlncRNAs) on the prognostic value and mechanism of immune infiltration of gastric cancer have not been studied. All information of gastric cancer (GC) patients was downloaded from the TCGA database.