Tolerogenic dendritic cells alleviate collagen-induced arthritis by regulating T-cell differentiation and inhibiting NLRP3-mediated apoptosis.

Objective: Tolerogenic dendritic cells (tolDCs) have emerged as a potential treatment for rheumatoid arthritis (RA). However, the detailed mechanism requires further investigation. In this study, we aimed to explore the effects of tolDCs on T-cell differentiation and NLRP3-mediated pyroptosis in a collagen-induced arthritis (CIA) rat model.

[Tolerogenic dendritic cells form microchimerism in vivo in collagen-induced arthritis rats].

Objective To find evidence of microchimerism formation following infusion of tolerogenic dendritic cells (tDCs) into collagen-induced arthritis (CIA) rats. Methods Bone marrow-derived DC precursor cells were induced into tDCs by granulocyte-macrophage colony- stimulating factor (GM-CSF), interleukin 4 (IL-4) and nuclear factor-κB oligonucleotide decoy (NF-κB ODN decoy) and then infused into CIA rats after labeling with 1, 1' -octadecyl-3, 3, 3', 3' -tetramethyltricarbocyanine iodide (DiR). At 4 hours, 24 hours, 5 days, 10 days and 15 days after cell infusion, the fluorescence intensity and distribution were observed by in vivo imaging system of small animals.

[Tolerogenic dendritic cells alleviate joint inflammation and arthropathy via reducing Th1 and Th17 cell proportion in CIA rats].

Objective To investigate the effects of tolerogenic dendritic cells (tolDCs) induced by nuclear factor κB oligodeoxynucleotide decoy (NF-κB ODN decoy) on Th1 cells, Th2 cells, Th17 cells and regulatory T cells (Tregs) and the intervention effects on collagen-induced arthritis (CIA) rats. Methods SD female rats used to establish CIA rat models were divided into four groups, including a CIA model group, a bovine type II collagen-decoy-dendritic cell (Col2-decoy DC) treatment group, a blank control group, and a Col2-decoy DC control group. On the 20th days after the first immunization, the rats were injected with tolDCs via the tail vein, and the rats were sacrificed on the 7th weeks.